C. acnes (ATCC 6919; American Type Culture Collection, Rockville, MD, USA) were obtained from the Korean Culture Center of Microorganisms (Seoul, Korea) and cultured at 37 °C on Brain Heart Infusion Medium (BD Diagnostics, Sparks, MD, USA) under anaerobic conditions at 37 °C until reaching OD600 = 1.0 (logarithmic growth phase). The log phase bacterial culture was centrifuged at 5000× g at 4 °C for 15 min. Subsequently, the culture supernatant was removed.
C acnes atcc 6919
Manufactured by American Type Culture Collection
Sourced in United States
C. acnes (ATCC 6919) is a bacterial strain obtained from the American Type Culture Collection (ATCC). It is a non-spore-forming, anaerobic, gram-positive bacterium. This strain is commonly used for microbiological research and applications.
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Lab products found in correlation
Brain heart infusion medium, by BD (1 mentions)
Syto9, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Dmem cell culture medium, by Thermo Fisher Scientific (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Bodipy fl c16, by Thermo Fisher Scientific (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Alexa fluor 647 conjugated dextran, by Thermo Fisher Scientific (1 mentions)
2 protocols using c acnes atcc 6919
1
C. acnes Cultivation Protocol
2
Optimal Culture and Characterization of C. acnes
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C. acnes (ATCC6919) was purchased from American Type Culture Collection (VA, USA). Soybean phosphatidylcholine (SPC, Phospholipon 80H) was purchased from American Lecithin Company (CT, USA). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and cholesterol were purchased from Sigma-Aldrich (MO, USA). SME was supplied by Croda (East Yorkshire, UK). DNS and PK were purchased from BioTools (Taipei, Taiwan). Alexa Fluor 647-conjugated dextran, SYTO9 and Bodipy FL C16 were purchased from Thermo Fischer Scientific (MA, USA). C. acnes culture medium reinforced clostridial medium (RCM) was purchased from Merck (Darmstadt, Germany), Cell culture medium DMEM and RPMI-1640 were purchased from Thermo Fischer Scientific.
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