Fcl pe100

RNA extracted from samples of total cellular lysate and polysome gradient fractions were quality-checked with the Bioanalyzer 2100, using the RNA6000Pico kit (Agilent Technologies, Santa Clara, CA, USA), as described above. DNA libraries were prepared using the MGIEasy RNA Directional Library Prep Set (MGI Tech, Shenzhen, China) according to the manufacturer’s instructions, and subjected to the next-generation sequencing (NGS) on the MGIseq-2000 platform, utilizing the 2x100 PE sequencing mode (FCL PE100, MGI Tech). All relevant procedures were performed in the SB RAS Genomics Core Facility (ICBFM SB RAS, Novosibirsk, Russia).

RNA extracted from total cell samples and sucrose gradient fractions with TRIzol (Invitrogen) was subsequently isolated by a Purelink RNA Micro Scale Kit (Invitrogen) and DNase treated (DNASE70, Sigma). The RNA integrity index (RIN) was assessed in a Bioanalyzer 2100 (Agilent Technologies) using the RNA 6000 Pico Kit, and RNA quantification was carried out using NanoDrop 1000 (Thermo Scientific) and Qubit (Invitrogen). For 1 µg of each isolated RNA sample, rRNA depletion was performed by RiboCop rRNA Depletion Kits for Human/Mouse/Rat V.2 (Lexogen). DNA libraries were prepared using the MGIEasy RNA Directional Library Prep Set (MGI Tech, China) according to the manufacturer's instructions and subjected to next-generation sequencing (NGS) on the MGIseq-2000 platform, using the 2 × 100 PE sequencing mode (FCL PE100, MGI Tech). All relevant procedures were performed in the SB RAS Genomics Core Facility (ICBFM SB RAS, Novosibirsk, Russia).

Total RNA was isolated from the cells producing target proteins, and the quality of the obtained samples was checked on the Bioanalyzer 2100 using the RNA6000Pico kit (Agilent Technologies, Santa Clara, CA, USA). Total RNA was isolated from cells using TRIzol reagent with subsequent treatment by the On-Column DNase I Digestion Set (Sigma, St. Louis, MO, USA) and polyA enrichment by the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, Ipswich, MA, USA).
DNA libraries were prepared from mRNA samples using the MGIEasy RNA Directional Library Prep Set (MGI Tech, Shenzhen, China) according to the manufacturer’s instructions and subjected to NGS on the MGISEQ-2000 platform, utilizing a sequencing set 2 × 100 PE (FCL PE100, MGI Tech). All relevant procedures were performed in SB RAS Genomics Core Facility (ICBFM SB RAS, Novosibirsk, Russia).