Anti opa1 612606

Manufactured by BD

Sourced in United States

Anti-OPA1 (612606) is a primary antibody that specifically targets the OPA1 protein. OPA1 is a mitochondrial protein involved in the regulation of mitochondrial fusion and cristae organization. The Anti-OPA1 antibody can be used for the detection and analysis of OPA1 in various biological samples and applications.

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Lab products found in correlation

Live cell imaging solution, by Thermo Fisher Scientific (3 mentions) Chloroquine c6628, by Merck Group (1 mentions) Thapsigargin t9033, by Merck Group (1 mentions) Anti phospho ulk1 ser757, by Cell Signaling Technology (1 mentions) Anti mfn1 sc 50330, by Santa Cruz Biotechnology (1 mentions) Anti ulk1, by Cell Signaling Technology (1 mentions) Anti tsc2, by Cell Signaling Technology (1 mentions) Anti map1lc3 lc3, by Cell Signaling Technology (1 mentions) Anti rps6kb p70s6k, by Cell Signaling Technology (1 mentions) Anti phospho rps6kb p70s6k thr389, by Cell Signaling Technology (1 mentions) Anti actb β actin, by Merck Group (1 mentions) Propidium iodide p4170, by Merck Group (1 mentions) Glucose solution, by Thermo Fisher Scientific (1 mentions) Mkeima red mito 7 plasmid, by Addgene (1 mentions) Mitotracker deep red fm, by Thermo Fisher Scientific (1 mentions) Mem non essential amino acids solution, by Thermo Fisher Scientific (1 mentions) Anti ulk1 20986 1 ap, by Proteintech (1 mentions) Anti drp1 ab184247, by Abcam (1 mentions) Mitosox red, by Thermo Fisher Scientific (1 mentions) Mitoview 633, by Biotium (1 mentions)

2 protocols using anti opa1 612606

Comprehensive Antibody Collection for Protein Analysis

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The following antibodies were obtained from Cell Signaling Technology (Beverly, MA): anti-pan-acetylated lysine (#9441), anti-MAP1LC3/LC3 (#4108), anti-RPS6KB/p70S6K (#9202), anti-phospho-RPS6KB/p70S6K (Thr389) (#9205), anti-TSC2 (#9442), anti-phospho-ULK1 (Ser 757) (#14202), anti-ULK1 (#8054), anti-phospho-DNM1L/Drp1 (Ser 616) (#3455), anti-phospho-MAPK3/ERK1-MAPK1/ERK2 (Thr 202/Tyr 204) (#9101), anti-MAPK3/ERK1-MAPK1/ERK2 (#9102), and anti-P-EIF2A/P-eIF2-α (Ser 51) (#9721). From Mitosciences: anti-RIESKE (MS305/C0183). From Abcam: anti-MFN2 (ab56889) and anti-HADHA (ab54477). From Sigma-Aldrich: anti-ACTB/β-actin (A5316). From Santa Cruz Biotechnology: anti-GFP (sc-9996), anti-TSC2 (sc-893), anti-TOMM20 (sc-17764), and anti-MFN1 (sc-50330). From Merck-Millipore: Nitrotyrosine 06-284. Other antibodies were used as follows: anti-mono- and polyubiquitinylated proteins FK2 conjugated with peroxidase (HRP) or FK2H from Enzo Life Sciences (BML-PW0150), anti-OPA1 (612606), and anti-DNM1L/Drp1 (611112) from BD Biosciences. Chloroquine C6628, propidium iodide (P4170), and thapsigargin (T9033) were from Sigma-Aldrich; rapamycin (553210) and resveratrol (R5010) were from Merck; and geneticin (G418) was from Santa Cruz (sc-29065).

Hernández M.G., Aguilar A.G., Burillo J., Oca R.G., Manca M.A., Novials A., Alcarraz-Vizan G., Guillén C, & Benito M. (2018). Pancreatic β cells overexpressing hIAPP impaired mitophagy and unbalanced mitochondrial dynamics. Cell Death & Disease, 9(5), 481.

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Mitochondrial Dynamics Regulation Assay

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Anti-ATPB (ATP synthase subunit β) pAb/mAb (ab128743/ab5432), anti-p62 (ab56416), anti-MFN1 (ab57602), anti-MFN2 (ab56889), anti-PINK1 (ab23707), anti-mitochondria [113-1] mAb (ab92824) and anti-DRP1 (ab184247), were purchased from Abcam (Cambridge, MA, USA), anti-ATG5 pAb (NB110-53818) from Novus Biologicals (USA), anti-OPA1 (612606) from BD (USA), anti-Gp78 pAb (16675-1-AP) and anti-ULK1 (20986-1-AP) from Proteintech (USA) and anti-LC3B pAb (2775S) from Cell Signaling. Anti-β-Actin mAb (A5441), tissue culture grade DMSO (D2650), Bafilomycin A1 (Cat# C1988) and CCCP (C2759) were purchased from Sigma-Aldrich (USA). mRFP‑GFP tandem fluorescent‑tagged LC3 (tfLC3) was a gift from Tamotsu Yoshimori (Addgene plasmid # 21074; www.addgene.org/21074) [54 (link)]. mKeima-Red-Mito-7 plasmid was a gift from Michael Davidson (Addgene plasmid #56018; www.addgene.org/56018). mCherry DRP-1 plasmid was a gift from Gia Voeltz (Addgene plasmid #49152 (https://www.addgene.org/49152/). MitoTracker Deep Red FM (M22426), Live Cell Imaging Solution (A14291DJ), Glucose Solution (A2494001), MitoSOX Red (M36008) and MEM Non-Essential Amino Acids Solution (11140050) were purchased from ThermoFisher (USA). MitoView 633 (#70055) was purchased from Biotium (USA).

Alan P., Vandevoorde K.R., Joshi B., Cardoen B., Gao G., Mohammadzadeh Y., Hamarneh G, & Nabi I.R. (2022). Basal Gp78-dependent mitophagy promotes mitochondrial health and limits mitochondrial ROS. Cellular and Molecular Life Sciences, 79(11), 565.

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