1 octanol

All chemicals used are of at least analytical reagent grade. Cobalt chloride hexahydrate (CoCl₂·6H₂O), metal ions studied for interferences, pyrocatechol violet and nitric acid were obtained from Carlo-Erba, France. 1-Undecanol, 1-dodecanol, dodecyltrimethyl ammonium bromide (DTAB), trimethyltetradecyl ammonium bromide (TTAB), and cethyltrimethyl ammonium bromide (CTAB) were purchased from Sigma-Aldrich (USA). 1-Octanol was obtained from Panreac (Spain). Methanol (HPLC grade) and acetonitrile (HPLC grade) were obtained from Merck (Germany). Acetone was purchased from Q-rac (Malaysia). Sodium sulphate anhydrous was purchased from Fluka (Japan). Sodium chloride, sodium hydrogen phosphate and sodium dihydrogen phosphate were purchased from Ajax Finechem (Australia). Aluminium chloride was obtained from Ajax Finechem Pty Ltd (New Zealand). All solutions were prepared using deionized water with the resistivity of 18.2 MΩ cm from RiOs™ Type I Simplicity 185 (Millipore Waters, USA). Stock solution of standard Co(ii) 100 mg L⁻¹ (or 1.695 mmol L⁻¹) was prepared and used throughout.

1-α-lecithin, soybean-cas 8002-43-5-calbiochem, and Chloroform c2432 > 99.5% were purchased from Merck (St. Louis, MO, USA). 1-Octanol and ethanol (96%) were purchased from PanReac AppliChem (Chicago, IL, USA). Chitosan (CS) (>75–85% deacetylation), glutaraldehyde (25%), acetic acid, hexane (99%), Tween 80, mineral oil, N-hydroxysuccinimide (NHS) (98%), N-[3-dimethylamino-propyl]-N’-ethyl carbodiimide hydrochloride (EDC) (98%), dimethyl sulfoxide (DMSO) (99%), rhodamine B (>95%) and polyethylene glycol were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Triton X-100 was purchased from Thermo Fisher (Waltham, MA, USA). Buforin II (BUF-II, TRSSRAGLQFPVGRVHRLLRK) was synthesized by Peptide Synthesis Facility at Pompeu Fabra University and GL Biochem Shanghai (Shanghai, China). Purification was performed by HPLC (>95%) and molecular weights were confirmed via mass spectrometry. Poly(methyl methacrylate) (PMMA) 3-mm thickness sheets and dichloromethane were purchased from local distributors (Bogotá, Colombia).

“Fotoditazin” was purchased from the “VETA-GRAND LLC” company (Moscow, Russia) as a 5 mg/mL concentrate. This solution was stable in the dark at 283 K for one year. “Fotoran e₆” was provided by the “RANFARMA” company (Krasnogorsk, Russia) as a solid powder mixed with polyvinylpyrrolidone as an appropriate carrier. Both PSs were also purchased in their pure solid state (final purity > 93%). 1-Octanol (Panreac, Barcelona, Spain, >98%) was dried with 4 Å molecular sieves and distilled under reduced pressure at 355 K. The phosphate saline buffer (PSB) pH = 7.4 (Agat-med, Moscow, Russia, for biochemical laboratories) was prepared by dissolving pure solid forms in one liter of freshly bidistilled water [14 (link),15 (link)]. 1,3-diphenylisobenzofuran (DPBF) (J&K Scientific Gmbh, Stadtkreis, Germany, >97%), as a selective singlet oxygen quencher, was used as supplied.