Blood samples for BACs were collected twice weekly, immediately after drinking sessions. The tail vein was nicked with a razor blade and 15–50 μL of blood was collected into heparinized capillary tubes, which was then centrifuged for 5 min to separate the plasma. An alcohol assay was run on the plasma samples using the Analox analyzer model GM7 MicroStat (Analox Instruments, Lunenburg, MA). Each individual blood sample was measured twice and the average of the two readings was used. To estimate the intra-assay variability, we calculated the percent variation of each duplicate from the mean, which was 9 ± 1.5%, and the difference between the two measurements, which was 4.9 ± 0.6 mg/dl (n = 78 duplicates). BACs were also measured in negative control samples of serum from water-drinking control mice. Baseline readings for negative control were on average 13.04 ± 0.79 mg/dl, which is in agreement with manufacturer’s specifications.
Analox analyzer model gm7 microstat
Manufactured by Analox Instruments
The Analox analyzer model GM7 MicroStat is a compact and portable gas monitoring device. It is designed to accurately measure the concentration of various gases in a sample. The core function of the GM7 MicroStat is to provide precise and reliable gas analysis.
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Lab products found in correlation
2 protocols using analox analyzer model gm7 microstat
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Blood Alcohol Concentration Measurement
2
Blood Alcohol Concentration Measurement
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Blood samples for BACs were collected twice weekly, immediately after drinking sessions. The tail vein was nicked with a razor blade and 15–50 μL of blood was collected into heparinized capillary tubes, which was then centrifuged for 5 min to separate the plasma. An alcohol assay was run on the plasma samples using the Analox analyzer model GM7 MicroStat (Analox Instruments, Lunenburg, MA). Each individual blood sample was measured twice and the average of the two readings was used. To estimate the intra-assay variability, we calculated the percent variation of each duplicate from the mean, which was 9 ± 1.5%, and the difference between the two measurements, which was 4.9 ± 0.6 mg/dl (n = 78 duplicates). BACs were also measured in negative control samples of serum from water-drinking control mice. Baseline readings for negative control were on average 13.04 ± 0.79 mg/dl, which is in agreement with manufacturer’s specifications.
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