All non-FRET confocal images were acquired on a Nikon A1R laser scanning confocal microscope with GaAsp detectors using a Plan Apo λ 100x 1.45 NA oil immersion objective (Nikon) using NIS-Elements (Nikon). Live cells were imaged in a temperature-controlled chamber (37 °C) at 5% CO2 at 1 frame every 2–3 sec. Dual-color videos were acquired as consecutive green–red images, and tricolor videos were acquired as consecutive green–red–blue images.
Plan apo λ 100x 1.45 na
Manufactured by Nikon
The Plan Apo λ 100x 1.45 NA is a high-performance objective lens designed for Nikon microscopes. It provides a magnification of 100x and a numerical aperture of 1.45, allowing for superior optical resolution and light-gathering capabilities. This lens is suitable for a wide range of microscopy applications that require detailed observation and analysis.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using plan apo λ 100x 1.45 na
1
Live Cell Confocal Imaging Protocol
2
Live Cell Confocal Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All non-FRET confocal images were acquired on a Nikon A1R laser scanning confocal microscope with GaAsp detectors using a Plan Apo λ 100x 1.45 NA oil immersion objective (Nikon) using NIS-Elements (Nikon). Live cells were imaged in a temperature-controlled chamber (37 °C) at 5% CO2 at 1 frame every 2–3 sec. Dual-color videos were acquired as consecutive green–red images, and tricolor videos were acquired as consecutive green–red–blue images.
3
Mitochondrial Dynamics Dissection via PAGFP
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All live cell confocal images were of Hela cells and acquired on a Nikon A1R confocal microscope with GaAsp detectors using a Plan Apo λ 100x 1.45 NA oil immersion objective (Nikon) using NIS-Elements (Nikon). Live cells were imaged in a temperature-controlled chamber (37°C) at 5% CO2 at 1 frame every 2-3s. For mito-PAGFP experiments, the matrix of a subpopulation of mitochondria were selectively labelled by localised photoactivation using a 405nm laser (100% for 4sec) in cells transfected with photoactivatable mitochondrial matrix marker mito-PAGFP and either control (mApple-Tom20) or mCherry MID51 (WT, p.R169W or p.A53V) and fluorescence intensity at a distal region (10µm from the site of photoactivation) was analysed.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!