Rotenone was purchased from Sigma-Aldrich (St Louis, MO, USA). Icariin was purchased from Nanjing Zelang Biological Technology Co., Ltd (Nanjing, China). Dulbecco's modified Eagle's medium (DMEM), horse serum, and fetal bovine serum (FBS) were supplied by Hyclone (Logan, UT). Antibodies against α-synuclein, Beclin-1, SQSTTM1/P62, mTOR, and Phospho-mTOR were purchased from Cell Signaling Technology (Boston, MA, USA). Antibody for LC3-I/II was purchased from Abcam (Cambridge, UK), and antibody for β-actin was purchased from Beyotime (Shanghai, China). LDH kit was purchased from Jiancheng Bioengineering Institute (Nanjing, China).
Antibody for β actin
Manufactured by Beyotime
Sourced in United Kingdom, China
The Antibody for β-actin is a laboratory product used for the detection and quantification of the β-actin protein, a widely expressed and highly conserved cytoskeletal protein. It can be used in various analytical techniques, such as Western blotting, immunocytochemistry, and immunohistochemistry, to study the expression and localization of β-actin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Beclin 1, by Cell Signaling Technology (1 mentions)
Ldh kit, by Nanjing Jiancheng (1 mentions)
Rotenone, by Merck Group (1 mentions)
Sqsttm1 p62, by Cell Signaling Technology (1 mentions)
Phospho mtor, by Cell Signaling Technology (1 mentions)
Anti bcl 2, by Santa Cruz Biotechnology (1 mentions)
Anti rabbit immunoglobulin g igg, by Merck Group (1 mentions)
2 protocols using antibody for β actin
1
Rotenone-Induced Parkinson's Disease Model
2
Western Blot Analysis of Lung Protein Expression
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The superior lobe of left lung was harvested from the rats and stored in -80 ℃ liquid nitrogen. Total proteins were extracted, and their concentrations were measured by protein assay. Cell lysates (20μg protein/lane) were separated on 10% sodium dodecyl sulphate polyacrylamide gel electrophoresis and transferred to polyvinylidene di uoride membranes, which were subsequently blocked with 5% skim milk in a phosphate-buffered saline with Tween (PBST) solution (100mM NaCl, 50mM Tris, 0.1% Tween-20, PH 7.5) for 1h at room temperature and probed overnight at 4 ℃ with appropriate primary antibodies (anti-Bcl-2, Santa Cruz, CA, USA). Horseradish peroxidase (HRP) conjugated anti-rabbit immunoglobulin G (IgG) and anti-rat IgG (Sigma-Aldrich, Saint Louis, USA) were used as secondary antibodies according to the primary antibodies. Immunoreactive bands were visualised by enhanced chemiluminescence. The antibody for β-actin (1:1000 dilution; Beyotime, China) was used as the endogenous control.
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