Plasma samples from blood drawn at the baseline study visit were used to analyze IL-6, hs-CRP, and fibrinogen. Participants were asked to fast for 8 h prior to the visit. Concentration of IL-6 in plasma was measured by a monoclonal antibody-based, high sensitive solid-phase ELISA method (R&D System, Inc.). The assay sensitivity is 0.039 pg/mL and the interassay CVs of the low-, medium-, and high-level quality control samples are 9.6%, 7.2% and 5.6%, respectively. hs-CRP was measured immunochemically in plasma using Siemens reagents on a nephelometer autoanalyzer (BNII). The assay sensitivity is 0.004 mg/dL and the interassay CVs of the low-, medium-, and high-level quality control samples were consistently < 3%. Fibrinogen was measured immunochemically in plasma using Siemens reagent on a nephelometer autoanalyzer (BNII). The assay sensitivity was 3.0 mg/dL and the CV consistently < 4%.
High sensitive solid phase elisa method
Manufactured by R&D Systems
The High sensitive solid-phase ELISA method is a laboratory technique used to detect and quantify specific proteins or other analytes in a sample. It utilizes antibodies coated on a solid surface, such as a microplate, to capture the target analyte. The captured analyte is then detected using a secondary antibody or enzyme-linked detection system, allowing for highly sensitive and accurate measurements.
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Lab products found in correlation
2 protocols using high sensitive solid phase elisa method
1
Inflammatory Biomarkers in Plasma Analysis
2
Inflammatory Biomarkers in Plasma Analysis
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Plasma samples from blood drawn at the baseline study visit were used to analyze IL-6, hs-CRP, and fibrinogen. Participants were asked to fast for 8 h prior to the visit. Concentration of IL-6 in plasma was measured by a monoclonal antibody-based, high sensitive solid-phase ELISA method (R&D System, Inc.). The assay sensitivity is 0.039 pg/mL and the interassay CVs of the low-, medium-, and high-level quality control samples are 9.6%, 7.2% and 5.6%, respectively. hs-CRP was measured immunochemically in plasma using Siemens reagents on a nephelometer autoanalyzer (BNII). The assay sensitivity is 0.004 mg/dL and the interassay CVs of the low-, medium-, and high-level quality control samples were consistently < 3%. Fibrinogen was measured immunochemically in plasma using Siemens reagent on a nephelometer autoanalyzer (BNII). The assay sensitivity was 3.0 mg/dL and the CV consistently < 4%.
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