Sorafenib, nilotinib, sunitinib, imatinib, gefitinib, lapatinib, dasatinib, PD-173074, selumetinib, crizotinib, tofacitinib and pazopanib were purchased from LC Laboratories (Woburn, Massachusetts, USA). Roswell Park Memorial Institute Medium (RPMI) and Geltrex basement membrane was purchased from Life Technologies (North Shore City, New Zealand). Met, P-Met (Y1234/Y1235), SRC, P-SRC (Y416), AKT, P-ATK (S473), cyclin D1, cleaved caspase 3, α-tubulin and EGFR antibodies were purchased from Cell Signaling Technology (Danvers, Massachusetts, USA). β-Tubulin was purchased from Sigma-Aldrich (Auckland, New Zealand).
Geltrex basement membrane
Manufactured by Thermo Fisher Scientific
Sourced in New Zealand
Geltrex is a basement membrane matrix derived from murine Engelbreth-Holm-Swarm (EHS) sarcoma cells. It is a soluble form of basement membrane that polymerizes into a three-dimensional gel at physiological temperatures.
Automatically generated - may contain errors
Lab products found in correlation
α tubulin, by Cell Signaling Technology (1 mentions)
β tubulin, by Merck Group (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Gefitinib, by LC Laboratories (1 mentions)
Lapatinib, by LC Laboratories (1 mentions)
Tofacitinib, by LC Laboratories (1 mentions)
Dasatinib, by LC Laboratories (1 mentions)
Imatinib, by LC Laboratories (1 mentions)
Crizotinib, by LC Laboratories (1 mentions)
Sorafenib, by LC Laboratories (1 mentions)
Sunitinib, by LC Laboratories (1 mentions)
Pazopanib, by LC Laboratories (1 mentions)
Nilotinib, by LC Laboratories (1 mentions)
Selumetinib, by LC Laboratories (1 mentions)
Pd 173074, by LC Laboratories (1 mentions)
Aphidicolin, by Thermo Fisher Scientific (1 mentions)
Long r3 igf 1, by Merck Group (1 mentions)
Rock inhibitor, by Selleck Chemicals (1 mentions)
Activin a, by R&D Systems (1 mentions)
Matrigel, by Corning (1 mentions)
2 protocols using geltrex basement membrane
1
Anticancer Drug Signaling Pathway Analysis
2
Feeder-free hESC UCSF4 Line Derivation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The hESC line UCSF4 was derived by Dr. Susan Fisher (UCSF), NIH registry number 0044 [Krtolica et al., 2011; Felfly and Klein, 2013] . UCSF4 was grown in feeder-free mTeSR media (STEM-CELL Technologies) with either Matrigel (Corning) or Geltrex basement membrane (Life Technologies). ROCK inhibitor (10 μ M , Selleckchem) was added to the cultures during passaging and was removed after 24 h. Activin A (10 ng/ml, R&D Systems), IGF (200 ng/ml, LONG R3 IGF-1, Sigma), and nucleosides (EmbroMax Nu-cleosides, Millipore) were added to cell cultures for 5 days before analysis. Aphidicolin (Fisher Scientific) treatment was performed by allowing the cells to attach and grow for 24 h, after which 0.1 or 0.2 μ M Aphidicolin was added for 16 h, followed by a 2-hour release from the drug.
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