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Sc 1924

Manufactured by Santa Cruz Biotechnology
Sourced in United States

Sc-1924 is a laboratory instrument produced by Santa Cruz Biotechnology. It is designed for conducting scientific research and analysis. The core function of Sc-1924 is to facilitate specific experimental procedures, but a detailed description cannot be provided while maintaining an unbiased and entirely factual approach.

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2 protocols using sc 1924

1

ELISA for Serum and Synovial Fluid

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ELISA plates were coated with 2 μg/ml capture antibody (sc-1924, Santa Cruz Biotech.) overnight at 4°C. Plates were then washed with PBST and blocked with 1% BSA PBST for one hour. Afterwards, serum or synovial fluid samples (diluted 1:10 in PBST) were added in duplicate and incubated at 37°C for one hour. Next, detection antibody (5 μg/ml) (sc-9061, Santa Cruz Biotech.) was added for 2 hours. The plates were then incubated with goat anti-rabbit IgG-HRP (sc-2030, Santa Cruz Biotech), followed by TMB substrate. For inter-plate standardization, 2 control samples were included on each plate.
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2

Annexin A2 Protein Identification Protocol

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A Donkey anti-goat IgG-HRP (sc-2033), a goat polyclonal antibody against AnnexinA2 (sc-1924), and a rabbit polyclonal antibody against GST (sc-459) were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA). Rabbit polyclonal anti-AnnexinA2 (ab75932) and goat anti-rabbit HRP (ab6721) were from Abcam (Cambridge, MA, USA). Rabbit polyclonal antibody against His6, Alexa fluor 488 labelled donkey anti-goat IgG(H + L) and alexa fluor 594 labelled donkey anti-rabbit IgG(H + L) were purchased from Invitrogen (Carlsbad, CA, USA). Plasmids were extracted using a Qiagen mini-prep kit from Qiagen (Mississauga, ON, Canada). PCR products were purified with a Qiagen PCR purification kit (Qiagen). All restriction enzymes were purchased from New England Biolab (Mississauga, ON, Canada). Fibrinogen, T4 DNA ligase, calf intestinal alkaline phosphatise and Taq polymerase were purchased from Invitrogen (Burlington, ON, Canada). LC/MS for protein identification was carried out at the McGill University and Genome Quebec Innovation Centre.
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