Following FISH or ICC, neurons were imaged using a Zeiss ELYRA PS.1 instrument (Carl Zeiss, Jena, Germany) at a resolution of 1028 by 1028 pixels, using a Zeiss 63x/1.4 NA Plan Apochromatic objective. Each fluorescent channel, 405, 488 and 561, was acquired using three pattern rotations with 3 translational shifts. The final SIM projection images were reconstructed using Zen 2013 (Carl Zeiss, Jena, Germany) and analyzed using ImageJ.
Na plan apochromatic objective
Manufactured by Zeiss
Sourced in Germany
The 63x/1.4 NA Plan Apochromatic objective is a high-performance microscope objective lens designed and manufactured by Zeiss. It features a magnification of 63x and a numerical aperture of 1.4, which enables the collection of a large amount of light and the creation of high-resolution images. The objective is plan apochromatic, meaning it is designed to provide excellent chromatic and spherical aberration correction across a wide range of the visible light spectrum. This objective is suitable for a variety of microscopy applications that require high-resolution, high-magnification imaging.
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Lab products found in correlation
3 protocols using na plan apochromatic objective
1
Super-Resolution Microscopy Imaging Protocol
2
Super-Resolution Imaging of Neurons
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Following immunocytochemistry, neurons were imaged using a Zeiss ELYRA PS.1 instrument (Carl Zeiss, Jena, Germany) at a resolution of 1028 by 1028 pixels, using a Zeiss 63X/1.4 NA Plan Apochromatic objective. Each fluorescent channel, 405, 488 and 561 were acquired using three pattern rotations with 3 translational shifts. The final SIM projection images were reconstructed using Zen 2013 (Carl Zeiss, Jena, Germany) and analyzed using ImageJ.
3
Super-Resolution Microscopy Imaging Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following FISH or ICC, neurons were imaged using a Zeiss ELYRA PS.1 instrument (Carl Zeiss, Jena, Germany) at a resolution of 1028 by 1028 pixels, using a Zeiss 63x/1.4 NA Plan Apochromatic objective. Each fluorescent channel, 405, 488 and 561, was acquired using three pattern rotations with 3 translational shifts. The final SIM projection images were reconstructed using Zen 2013 (Carl Zeiss, Jena, Germany) and analyzed using ImageJ.
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