Cy3 labeled goat anti rabbit igg

Manufactured by Thermo Fisher Scientific

Sourced in United States

Cy3-labeled goat anti-rabbit IgG is a secondary antibody conjugated with the fluorescent dye Cy3. It is designed to detect and bind to rabbit immunoglobulin G (IgG) in various immunoassay applications.

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Lab products found in correlation

Fluorescence microscope, by Olympus (2 mentions) Fitc labeled goat anti mouse igg, by Abcam (2 mentions) Cy3 labeled goat anti mouse igg, by Thermo Fisher Scientific (2 mentions) α sma, by Affinity Biosciences (1 mentions) Hsp110, by Affinity Biosciences (1 mentions) α sma, by ABclonal (1 mentions) Beclin1, by ABclonal (1 mentions) Alexa fluor 488 donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Glial fibrillary acidic protein (gfap), by Abcam (1 mentions) Neuronal nuclei (neun), by Abcam (1 mentions) Nf κb p p65 antibody, by Affinity Biosciences (1 mentions) Bx53 microscope, by Olympus (1 mentions) Bovine serum albumin (bsa), by Sangon (1 mentions) Slc27a3, by Proteintech (1 mentions) Stau1, by Affinity Biosciences (1 mentions) Dfc 500 digital camera, by Leica (1 mentions) A 21424, by Thermo Fisher Scientific (1 mentions) A19653, by ABclonal (1 mentions) Fitc labeled goat anti rabbit igg, by Abcam (1 mentions) A27039, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Cy3-labeled secondary antibody goat anti-rabbit IgG Goat anti-rabbit IgG-Cy3 Goat anti-rabbit CY3 Anti-rabbit Cy3 IgG Rabbit

9 protocols using cy3 labeled goat anti rabbit igg

Immunofluorescent Analysis of CD4+ Cells in Liver Tissue

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The liver tissue was dehydrated in 70% (2 h), 80% (overnight), 90% (2 h), 100% I (1 h), and 100% II (1 h) ethanol successively. Dehydrated liver tissue was embedded through wax and
subsequently cut into 5 µm sections. After antigen repair, liver tissue sections were incubated with antibodies. Primary antibody CD4 was purchased from ABclonal, China.
Secondary antibody was Cy3-labeled goat anti-rabbit IgG (Invitrogen, Carlsbad, CA, USA). The sections were stained with DAPI (Aladdin). The staining was observed under a microscope.

Zhu J., Chen H., Cui J., Zhang X, & Liu G. (2023). Oroxylin A inhibited autoimmune hepatitis-induced liver injury and shifted Treg/Th17 balance to Treg differentiation. Experimental Animals, 72(3), 367-378.

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Immunofluorescence Analysis of Lung Tissue

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The section (5 µm) from lung tissues were washed with PBS and separately co-incubated with HSP110 (Affinity) and YAP (Proteintech), HSP110 (Affinity) and alpha smooth muscle actin (α-SMA) (Affinity), YAP (Proteintech) and α-SMA (Abclonal), proliferating cell nuclear antigen (PCNA) (Abclonal) and α-SMA (Affinity), and Beclin1 (Abclonal) and α-SMA (Affinity) antibodies overnight at 4 °C. After washing, the sections were subject to second antibodies Cy3-labeled goat anti-rabbit IgG (Beyotime; Invitrogen, USA) and FITC-labeled goat anti-mouse IgG or anti-rabbit IgG (Beyotime; Abcam, UK) incubation at room temperature for 1.5 h. For cell immunofluorescence staining, fixed glass coverslips with cells were incubated with primary antibodies α-SMA (Affinity), Beclin1 (Abclonal) and YAP (Proteintech) overnight at 4 °C. Then the cells were subsequently incubated with fluorescent secondary antibodies Cy3-labeled goat anti-mouse IgG or anti-rabbit IgG (Beyotime) at room temperature for 1 h. The sections and coverslips then were incubated with DAPI (Aladdin, China) for counterstain and pictures were taken with fluorescence microscope (Olympus).

Liu H., Zhang S., Liu Y., Ma J., Chen W., Yin T., Li T., Liang B, & Tao L. (2022). Knockdown of HSP110 attenuates hypoxia-induced pulmonary hypertension in mice through suppression of YAP/TAZ-TEAD4 pathway. Respiratory Research, 23, 209.

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Immunofluorescence Staining of Neural Markers

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Immunofluorescence was carried out by standard protocol as previously described [22 (link)]. We used primary antibodies against Tuj1 (1 : 400; CST, Danvers, MA, USA), MAP-2 (1 : 200, CST), ChAT (1 : 200; Abcam, Cambridge, MA, USA), GFAP (1 : 500; Abcam), neuronal nuclear antigen (NeuN, 1 : 300; Abcam), Caspase-3 (1 : 400; CST), and Nrf2 (1 : 200; R&D, Minneapolis, MN, USA). Alexa Fluor® 488 donkey anti-mouse IgG (1 : 200; Invitrogen, Carlsbad, CA, USA) and Cy3-labeled goat anti-rabbit IgG (1 : 300; Invitrogen) were used as second antibodies.

Zhan J., Li X., Luo D., Yan W., Hou Y., Hou Y., Chen S., Luan J., Zhang Q, & Lin D. (2021). Polydatin Attenuates OGD/R-Induced Neuronal Injury and Spinal Cord Ischemia/Reperfusion Injury by Protecting Mitochondrial Function via Nrf2/ARE Signaling Pathway. Oxidative Medicine and Cellular Longevity, 2021, 6687212.

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Immunofluorescence Staining Protocol for Tissue Analysis

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Immunofluorescence (IF) staining was constructed by the method described in many studies. The tissue was embedded in paraffin and cut into 5-µm sections beforehand. All slides were deparaffinized, rehydrated in xylene, 100%, 95%, 85%, 75% ethanol, and PBS, and constantly stained with 10% antigen retrieval solution for 10 min. BSA (1%, Sangon) as the blocking buffer was incubated at room temperature for 15 min. The primary antibody was added and incubated at 4°C overnight. Then, the secondary antibody, and 4′,6-diamidino-2-phenylindole (DAPI) (Aladdin) were added to this procedure. The antibodies used in this section involved as follows: NF-κB p-p65 antibody (1:200, Affinity, Jiangsu, China), CD68 (1:50, Santa Cruz, Santa Cruz, CA, USA), iNOS (1:100, ABclonal), Cy3 labeled goat anti-mouse IgG (1:200, Invitrogen, Carlsbad, CA, USA), Cy3 labeled goat anti-rabbit IgG (1:200, Invitrogen), FITC labeled goat anti-mouse IgG (1:200, Abcam, Cambridge, UK). The immunofluorescence images were taken and preserved using an OLYMPUS-BX53 microscope (OLYMPUS, Tokyo, Japan).

Song Z., Wang X., He L., Chen L., Ren Z, & Song S. (2022). Suppression of lysosomal-associated protein transmembrane 5 ameliorates cardiac function and inflammatory response by inhibiting the nuclear factor-kappa B (NF-κB) pathway after myocardial infarction in mice. Experimental Animals, 71(4), 415-425.

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Histological Lung Tissue Analysis

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After treatment, lung tissues were collected. Paraffin embeded lung tissues were cut into slices for histological staining. HE staining was performed according to the manufacturer’s instructions (Solarbio). The mean alveolar septal thickness (MAST), mean linear intercept (MLI), and destructive index (DI) were calculated. Immunohistochemistry staining was conducted using primary antibodies for SLC27A3 (Proteintech), STAU1 (Affinity), CD57 (Affinity), and CD8a (Affinity). Immunofluorescence staining was performed using primary antibodies for CD19 (Affinity) and CD27 (Santa), and secondary antibodies including Cy3-labeled goat anti-rabbit IgG (Invitrogen) and FITC-labeled goat anti-mouse IgG (Abcam).

Zhang Y., Xia R., Lv M., Li Z., Jin L., Chen X., Han Y., Shi C., Jiang Y, & Jin S. (2022). Machine-Learning Algorithm-Based Prediction of Diagnostic Gene Biomarkers Related to Immune Infiltration in Patients With Chronic Obstructive Pulmonary Disease. Frontiers in Immunology, 13, 740513.

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Immunofluorescence Analysis of SIRT3 in Rat Lung Tissue

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Lung tissues obtained from the rats were processed into 5-µm-thick sections and incubated with anti-SIRT3 overnight at 4°C, as in the case of the IHC. Next, the tissues
were kept in Cy3-labeled goat anti-rabbit IgG (1:200; Invitrogen, Thermo Fisher Scientific) for 1 h. Finally, all samples were stained with 2-(4-amidinophenyl)-6-indolecarbamidine
dihydrochloride (DAPI; Aladdin, Shanghai, China) and visualized with a fluorescence microscope (×400 magnification; Olympus).

Xu C., Han J., Jia D., Cai J., Yuan J, & Ge X. (2023). Sirtuin3 confers protection against acute pulmonary embolism through anti-inflammation, and anti-oxidative stress, and anti-apoptosis properties: participation of the AMP-activated protein kinase/mammalian target of rapamycin pathway. Experimental Animals, 72(3), 346-355.

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Indirect Immunofluorescence Detection of Spore Surface Protein

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Using the indirect immunofluorescence method, based on the previous reports [42 (link),43 (link)], we detected whether VP56-2 was displayed on the surface of the spore. Briefly, firstly, we fixed 200 μL of the obtained B. s-CotC-VP56-2 and B. s-CotC spore suspension on a glass slide. Then, the slide with normal goat serum was incubated overnight at 4 °C. Next, we used mouse anti-rVP56 serum (1:200 dilution) as the primary antibody, incubated the slide for 2 h at room temperature and used negative mouse serum as a control. After washing thoroughly, cyanine3 (Cy3)-labeled goat anti-rabbit IgG (1:500 diluted with PBST, Invitrogen, Carlsbad, California, USA) was used as the secondary antibody for incubation for 1 h at RT (in the dark). The samples were incubated for 3–5 min with a DNA staining solution-4’, 6-diamidino-2-phenylindole (DAPI). Finally, the images were captured under a fluorescent microscope (Leica DFC500 Digital Camera, Barnack, Germany) in the dark.

Gao Y., Huo X., Wang Z., Yuan G., Liu X., Ai T, & Su J. (2021). Oral Administration of Bacillus subtilis Subunit Vaccine Significantly Enhances the Immune Protection of Grass Carp against GCRV-II Infection. Viruses, 14(1), 30.

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Immunohistochemical Analysis of Brain and Microglial Cells

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Brain tissue was embedded in paraffin and then sectioned. BV2 microglial cells were washed with phosphate buffered saline (PBS) and fixed with 4% buffered paraformaldehyde (Sinopharm). Brain tissue and cells were then incubated overnight at 4°C with primary antibodies: FERMT1 (1:100 dilution; 22215-1-AP; proteintech, Hubei, China), ionized calcium binding adapter molecule 1 (Iba1; 1:50 dilution; Sc-32725; Santa, Shanghai, China) and NF-κB p65 (1:100 dilution; A19653; ABclonal, Hubei, China). Secondary antibodies were added and incubated for 1 h at room temperature. Secondary antibody: FITC-labeled goat anti-rabbit IgG (1:200 dilution; ab6717; Abcam, Cambridge, MA, USA), Cy3-labeled goat anti-mouse IgG (1:200 dilution; A-21424; Invitrogen) and Cy3-labeled goat anti-rabbit IgG (1:200 dilution; A27039; Invitrogen). Finally, nuclei were stained with DAPI (D106471-5mg, Aladdin). Tissue and cells were observed under an inverted fluorescence microscope (Olympus).

Wei J., Yin J., Cui Y., Wang K., Hong M, & Cui J. (2023). FERM domain containing kindlin 1 knockdown attenuates inflammation induced by intracerebral hemorrhage in rats via NLR family pyrin domain containing 3/nuclear factor kappa B pathway. Experimental Animals, 72(3), 324-335.

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Alloxan-Induced Neuronal Damage in SK-N-SH Cells

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The SK-N-SH cells plated on poly L-lysine-coated 8-chamberred glass slides were subjected to various treatment. One was that different concentration of alloxan (0, 4, 6 mM) was added to each chamber and incubated at 37 °C for 12 h. And for another, the cells was preincubated 20μM NAG-Ae for 12 h before 6mM alloxan was added. Then, cells were fixed for 20 min in 10% neutral formaldehyde on ice. After permeabilization with 0.5% Triton X-100 for 20 min, cells were treated with 3% hydrogen peroxide to block endogenous peroxidase and incubated with 5% BSA for 20 min. Then, cells were incubated with primary antibodies SMI31/NF160, RL2/NF160 at 4 °C overnight. After that, cells were washed with PBS and incubated with secondary antibodies, FITC-labeled goat anti-mouse IgG, Cy3-labeled goat anti-rabbit IgG (Invitrogen, NM, USA) for 30 min at 37 °C. Then the glides were covered with anti-fade mounting medium.

Ding N., Peng P., Chu Y.J., Wang J.J., Chen S.Y., Arulthas R, & Deng Y.Q. (2018). The effects of O-GlcNAc alteration on Alzheimer-like neurodegeneration in SK-N-SH cells. Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia, 162(3).

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