Neuro-2a (N2a) cell line was acquired from the Collection (ATCC, Manassas, VA, United States) whereas 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT), bovine serum albumin (BSA), penicillin, streptomycin, 2,2-diphenyl-1-picrylhydrazyl (DPPH), Trolox, Monoamine oxidase A (MAO-A), Trizma base, 4-aminoantipyrine, 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), tyramine, levodopa (L-DOPA), horseradish peroxidase, 2,2′-azobis (2-methyl-propionamidine)-di-hydrochloride (AAPH), hydrogen peroxide (30%), vanillic acid, tyrosinase, 2,4,6-Tris (2-pyridyl)-1,3,5-triazine (TPTZ), galantamine, 2′,7′-Dichlorodihydrofluorescein Diacetate (DCFH-DA), xanthine, ferrous sulfate (FeSO4), acetylcholinesterase (AChE), acetylthiocholine iodide (ATCI) were obtained from Sigma-Aldrich (Madrid, Spain). Clorgyline and α-kojic were from Cymit química (Barcelona, Spain). Sodium carbonate (Na2CO3), HCl, NaCl, dimethyl sulfoxide (DMSO), methanol, and potassium phosphate were supplied from Panreac (Barcelona, Spain). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), Nitroblue Tetrazolium (NBT) and xanthine oxidase were purchased from Vidrafoc (Barcelona, Spain) and iron chloride (FeCl3) from Laboaragon (Zaragoza, Spain). All reagents used were of analytical quality.
Levodopa l dopa
Manufactured by Merck Group
Sourced in United States
Levodopa (L-DOPA) is a pharmaceutical ingredient commonly used in the production of drugs. It is a naturally occurring amino acid that serves as a precursor to the neurotransmitters dopamine, norepinephrine, and epinephrine. Levodopa is a key component in the treatment of Parkinson's disease and other neurological conditions.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase, by Merck Group (2 mentions)
Tyramine, by Merck Group (2 mentions)
Acetylthiocholine iodide, by Merck Group (2 mentions)
Tyrosinase, by Merck Group (2 mentions)
Sodium chloride (nacl), by ITW Reagents (2 mentions)
4 aminoantipyrine, by Merck Group (2 mentions)
Galantamine, by Merck Group (2 mentions)
Acetylcholinesterase ache, by Merck Group (2 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Hydrogen peroxide, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Trolox, by Merck Group (1 mentions)
Trizma base, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by ITW Reagents (1 mentions)
Ferrous sulfate feso4, by Merck Group (1 mentions)
2 2 azobis 2 methylpropionamidine dihydrochloride, by Merck Group (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions)
Methanol, by ITW Reagents (1 mentions)
Monoamine oxidase a mao a, by Merck Group (1 mentions)
6 protocols using levodopa l dopa
1
Neuro-2a Cell Assays and Antioxidant Screening
2
Neuroprotective Effects of Curcumin Derivatives
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FeCl3•6H2O 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2'-Azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), and MB were procured from J&K Scientific Co., Ltd (Beijing, China). Polyvinylpyrrolidone (PVP, MW =8000) was purchased from Titan Scientific Co., Ltd (Shanghai, China). Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Laboratories (Kumamoto, Japan). The Calcein-AM/PI double staining kit and 2,7-dichlorodi-hydrofluorescein diacetate (DCFH-DA) were procured from Beyotime Biotechnology Co., Ltd. (Beijing, China). 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was obtained from MedChemExpress (NJ, USA). Cur, Indocyanine Green (ICG), 1-methyl-4-phenylpyridinium ion (MPP+), and Levodopa (L-DOPA) were obtained from Sigma-Aldrich (St. Louis, USA). Reactive oxygen species (ROS), adenosine triphosphate (ATP), and malondialdehyde (MDA) ELISA kits were purchased from R&D Systems, Inc., USA.
3
Enzymatic Assays for Pharmacological Screening
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The chemical reagents of α-glucosidase from Saccharomyces cerevisiae, p-nitrophenyl glucopyranoside (pNPG), lipase type II from porcine pancreas, p-nitrophenyl butyrate (pNPB), vanillic acid, 4-aminoantipyrine, horseradish peroxidase, tyramine, MAO-A, galantamine, acetylthiocholine iodide (ATCI), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), Tris, acetylcholinesterase (AChE), levodopa (L-DOPA), and tyrosinase were acquired through Sigma-Aldrich (Madrid, Spain); clorgyline and α-kojic acid were from Cymit quimica (Barcelona, Spain); MgCl2·6H2O, HCl, NaCl, and potassium phosphate were from Panreac (Barcelona, Spain).
4
Melanoma Cell Cytotoxicity Assay
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B16F10 melanoma cells were seeded at a density of 5 × 104 cells/well in 24-well plates. Further, the cells were treated with FFAR2 selective antagonist GLPG0974 (0.1 µM, GLPG) and PA (4 mM) and incubated at 37 °C in 5% CO2 for 24 h. Cells were trypsinized and lysed with RIPA buffer (Thermo Fisher Scientific, NJ, USA). The lysed cells were frozen at -80 °C and thawed twice followed by centrifugation at 12,000 rpm for 10 min. The supernatant was mixed with 1 μg/mL of Levodopa (L-DOPA, Sigma) in 2:1 ratio in a 96-well plate, incubated at room temperature (RT) for 1 h and OD at 475 nm was detected27 (link).
5
Evaluating Drug-Induced Rotational Asymmetry
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Drug‐induced rotational asymmetry was assessed in rotometer bowls, as described previously (Inden et al., 2006 ). In brief, the numbers of full body turn rotations in the ipsilateral and contralateral directions were counted for 60 min after the drug injection. The rotational behavior induced by levodopa (L‐DOPA) (10 mg/kg, i.p.; Sigma) was measured 10 days after the operation in rats that were treated with an aromatic L‐amino acid decarboxylase (AADC) inhibitor, benserazide (6.25 mg/kg, i.p.; Sigma), 15 min before L‐DOPA injection. Seven days later, the rotational behavior induced by F‐DOPA (10 mg/kg, i.p.) or F‐L‐tyrosine (10 mg/kg, i.p.) was measured in rats that received benserazide (6.25 mg/kg, i.p.) 15 min before the injection.
6
Electrochemical Analysis of Neurotransmitters
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Sodium hydroxide, sulfuric acid (99.99 %), ethanol (≥99.88 %), sodium chloride, components of buffer solutions, Tris-(2-carboxyethyl)phosphine hydrochloride (TCEP), cysteamine, dopamine, norepinephrine, catechol, and levodopa (L-dopa) were from Sigma-Aldrich (Germany). All chemicals were used as received without further purification. Ultrapure water from Milli-Q ® water purification system (18 MΩ.cm, Millipore, Bedford, MA, USA) was used throughout the work.
Stock solutions of each NT in a 20 mM phosphate buffer solution containing 0.15 M NaCl (PBS), pH 7.4, were freshly prepared before measurements and protected from light.
All RNA and DNA sequences were synthesized, purified through HPLC and mass checked by
Stock solutions of each NT in a 20 mM phosphate buffer solution containing 0.15 M NaCl (PBS), pH 7.4, were freshly prepared before measurements and protected from light.
All RNA and DNA sequences were synthesized, purified through HPLC and mass checked by
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