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Xl30 feg sem

Manufactured by Thermo Fisher Scientific
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The XL30 FEG-SEM is a field emission gun scanning electron microscope (FEG-SEM) manufactured by Thermo Fisher Scientific. It provides high-resolution imaging and analysis capabilities for a wide range of materials and applications.

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Lab products found in correlation

Nanowizard 2, by Bruker (2 mentions) Np s10 probes, by Bruker (2 mentions) Pin stubs, by Ted Pella (1 mentions) 24 well ultra low attachment plate, by Corning (1 mentions) Desk 4, by Denton (1 mentions) Aramis system, by Horiba (1 mentions) Nanowizard 2 afm, by Bruker (1 mentions) Xe 100, by Park Systems (1 mentions) K550x sputter coater, by Emitech (1 mentions) Eos 60d, by Leica (1 mentions) Cam 200, by Biolin Scientific (1 mentions) Glutaraldehyde, by Agar Scientific (1 mentions)

Close spelling variants of this product

FEI XL30 FEG-SEM XL30 SEM-FEG scanning XL30 Environmental SEM-FEG XL30 FEG XL30 SEM FEG-SEM

16 protocols using xl30 feg sem

1

Fixation and Dehydration Protocol for SEM

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Cells were seeded at 12,000 cells/cm2 on functionalized leaves and glass coverslip. After 72 h, they were fixed with 2.5% glutaraldehyde in 1X PBS overnight at 4°C. Samples were dehydrated in increasing concentrations of ethanol (50, 70, 85, 95, 95, 100, 100%) for 1 h each and left overnight in 100% ethanol at 4°C. Samples were left to dry on pin stub mounts (12.7 mm × 8 mm, Ted Pella) and sputter coated with gold (1 0 nm) prior imaging to the Eyring Materials Center at Arizona State University with a SEM-FEG XL30 (FEI).
Lacombe J., Harris A.F., Zenhausern R., Karsunsky S, & Zenhausern F. (2020). Plant-Based Scaffolds Modify Cellular Response to Drug and Radiation Exposure Compared to Standard Cell Culture Models. Frontiers in Bioengineering and Biotechnology, 8, 932.
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2

Decellularized Scaffold Characterization

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Decellularized scaffolds were fixed with 2.5% glutaraldehyde in PBS overnight at 4 °C. Samples were then dehydrated in increasing concentrations of ethanol (50, 70, 85, 95, 95, 100, 100%) for one hour each and left overnight in 100% ethanol at 4 °C. Samples were mounted on pin stubs (12.7 mm × 8 mm, Ted Pella) and sputter coated with gold (10 nm) under vacuum for direct SEM imaging or with carbon for EDS. Samples were imaged at the Eyring Materials Center at Arizona State University with a SEM-FEG XL30 (FEI) and EDS data acquired using Genesis Spectrum software version 5.21. (EDAX Inc., New Jersey, USA https://www.edax.com/).
Harris A.F., Lacombe J., Liyanage S., Han M.Y., Wallace E., Karsunky S., Abidi N, & Zenhausern F. (2021). Supercritical carbon dioxide decellularization of plant material to generate 3D biocompatible scaffolds. Scientific Reports, 11, 3643.
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3

Ultrastructural Analysis of Fungal Conidia

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For scanning electron microscopy, conidia were harvested from strains grown on GMM agar for 5 days and fixed with osmium tetroxide for 72 h. Fixed conidia were sputter coated with gold and visualized using an accelerating voltage of 7 kV on a FEI XL30 SEM-FEG. For transmission electron microscopy, conidia were harvested from strains grown on GMM agar for 5 days and then fixed with 4% formaldehyde and 2% glutaraldehyde in 1x PBS for 24 h at 4°C. Samples were washed twice with PBS for 10 min and then stained for an hour with osmium tetroxide. Conidia were washed twice with PBS and once with 0.1N acetate buffer for 10 min. Then, conidia were stained with 0.5% uranyl acetate for an hour. After staining, conidia were washed twice using 0.1N acetate buffer for 10 min each. Samples were dehydrated using serial washes of 30%, 50%, 70%, 90% and 100% ethanol twice for 10 min at each concentration. Samples were embedded using the SPURR Low Viscosity Embedding Kit. Samples were then thin sectioned and stained using 1% uranyl acetate and 0.4% lead citrate. Conidia were visualized using an accelerating voltage of 80 kV on a FEI Tecnai G2 Twin.
Vargas-Muñiz J.M., Renshaw H., Richards A.D., Lamoth F., Soderblom E.J., Moseley M.A., Juvvadi P.R, & Steinbach W.J. (2015). The Aspergillus fumigatus Septins Play Pleiotropic Roles in Septation, Conidiation, and Cell Wall Stress, but are Dispensable for Virulence. Fungal genetics and biology : FG & B, 81, 41-51.
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4

Fabrication of Porous PCL Scaffolds

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Scaffolds were woven from multifilament PCL yarns (EMS-Griltech, Domat, Switzerland) using a custom-built weaving machine, as previously described [7 (link)]. For this study, 7 layers of yarns were axially oriented in alternating x and y directions with a third set of fibers passing through the thickness of the structure (z-direction). Total void space within the scaffold was ~61% with interconnected rectangular pores measuring approximately 350 μm × 250 μm × 100 μm. Scaffolds were treated with 4N NaOH for 16 hours to increase surface hydrophilicity, rinsed in DI H2O, and dried. Scaffolds were subsequently heat set for 10 min at 57°C in DI H2O. Dried scaffolds were then punched using a trephine to obtain uniform 5 mm disks. For scanning electron microscopy, disks were mounted, sputter-coated with gold, and imaged with a scanning electron microscope (FEI XL30 SEM-FEG, Eindhoven, Netherlands). For tissue engineering experiments, disks were ethylene-oxide sterilized in 24 well ultra-low attachment plates (Corning, Corning, NY) prior to use.
Glass K.A., Link J.M., Brunger J.M., Moutos F.T., Gersbach C.A, & Guilak F. (2014). Tissue-engineered cartilage with inducible and tunable immunomodulatory properties. Biomaterials, 35(22), 5921-5931.
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5

Microparticle Characterization via SEM

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Microparticles were extracted from droplets and resuspended in water. An aliquot of sample (5 µL) was drop-cast onto a silicon wafer attached to an aluminum stub with double-sided adhesive carbon tape and allowed to air dry for at least 4 hours. The dried samples were sputter-coated with gold for 250 s (Denton Desk IV, Moorestown, NJ) and then imaged with an FEI XL30 SEM-FEG at 7 kV. Mesoparticles were crosslinked in bulk and diluted 1:100 in water prior to sample preparation.
Costa S.A., Simon J.R., Amiram M., Tang L., Zauscher S., Brustad E.M., Isaacs F.J, & Chilkoti A. (2017). Photocrosslinkable Unnatural Amino Acids Enable Facile Synthesis of Thermoresponsive Nano- to Micro-gels of Intrinsically Disordered Polypeptides. Advanced materials (Deerfield Beach, Fla.), 30(5), 10.1002/adma.201704878.
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6

Scanning Electron Microscopy of Mouse Ear

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Ear pinnae were dissected from mice 15 min after PSA and fixed in 4% PFA overnight at 4˚C. Then ear tissues were dehydrated with an ethanol series and finally dried in hexamethyldisilane (HMDS) (Sigma, 440191). Ear samples were coated with gold for 600s employing the SPUT6 Vacuum Sputter Coater (Kurt Lesker PVD 75) before being imaged with a scanning electron microscope (FEI XL30 SEM-FEG).
Bao C., Chen O., Sheng H., Zhang J., Luo Y., Hayes B.W., Liang H., Liedtke W., Ji R.R, & Abraham S.N. (2023). A Mast Cell-Thermoregulatory Neuron Circuit Axis Regulates Hypothermia in Anaphylaxis. Science immunology, 8(81), eadc9417.
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7

Raman and SEM Analysis of Nanostructures

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Raman spectroscopy was performed with a Horiba Jobin Yvon LabRam ARAMIS system operating with a 633 nm HeNe laser. The details of the calculation of the ratio of the D and G band intensities and of the nanocrystalline domain size are described elsewhere [23 ]. Scanning electron microscopy (SEM) was performed using a FEI XL30 SEM-FEG.
Henry P.A., Raut A.S., Ubnoske S.M., Parker C.B, & Glass J.T. (2014). Enhanced electron transfer kinetics through hybrid graphene-carbon nanotube films. Electrochemistry communications, 48, 103-106.
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8

Nanomechanical Analysis of Collagen Fibrils

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Atomic force microscopy (AFM) imaging (XE-100; Park Systems, Seoul, South Korea and Nanowizard II; JPK Instruments, Berlin, Germany) was performed in contact mode using NP-S10 probes (Bruker, Santa Barbara, CA, USA) with a spring constant of 0.3 N m−1 at a scanning rate of 1.0 Hz or above. All AFM images were acquired directly from the histologic sections without any further processing or rehydration. Four sections were taken from each volunteer biopsy and a series of AFM images captured at four locations in the reticular dermis with scan sizes of 5×5, 10×10 and 40×40 μm2. Scanning electron microscopy (SEM; XL-30 FEG SEM; FEI, Eindhoven, the Netherlands) was performed on sections coated with Au/Pd, using an acceleration voltage of 5 kV. Nanomechanical analysis was performed using a Nanowizard II AFM (JPK Instruments) system mounted on an Olympus IX71 (Olympus Corporation, Tokyo, Japan) inverted microscope. All AFM mechanical measurements were acquired directly on the histologic sections without any further processing or rehydration. All force–distance curves were acquired using RFESPA probes (Bruker) with a spring constant k=3 N m−1 used to indent radially individual collagen fibrils. All forces curves were then analyzed using a custom Matlab® algorithm, following the Oliver–Pharr modeling method to calculate the Young’s modulus (E) for fibrils.30
Ahmed T., Nash A., Clark K.E., Ghibaudo M., de Leeuw N.H., Potter A., Stratton R., Birch H.L., Enea Casse R, & Bozec L. (2017). Combining nano-physical and computational investigations to understand the nature of “aging” in dermal collagen. International Journal of Nanomedicine, 12, 3303-3314.
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9

Biofilm Structural Analysis via SEM

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Biofilms were grown on 0.6% agar plates for 2 days as described above. The region of the agar containing a biofilm (~ 2 cm × 2 cm) was separated from the remainder of the agar plate, transferred to a piece of glass, and placed horizontally in a 50 mL conical tube and frozen at −80°C overnight followed by overnight lyophilization (Millrock Technology, BT85A-A). The biofilm samples were sliced with a razor blade to expose blisters, sputter-coated with a 5 nm layer of Pd (VCR IBS/TM200S ion beam sputterer), adhered to an upright SEM stub with conductive tape, and imaged with a scanning electron microscope (FEI XL30 FEG-SEM).
Yan J., Fei C., Mao S., Moreau A., Wingreen N.S., Košmrlj A., Stone H.A, & Bassler B.L. (2019). Mechanical instability and interfacial energy drive biofilm morphogenesis. eLife, 8, e43920.
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10

Analyzing Androconial Scale Morphology

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The detailed morphology of androconial scales was determined using a Field Emission Scanning Electron Microscope. Three males and two females of H. melpomene from Ecuador were used for this analysis. The overlap grey scale region was dissected out from both hind and forewings and attached to aluminium stubs with carbon tabs and subsequently coated with 20 nm of gold using a Quorum/Emitech sputter coater. The gold-coated regions were then viewed in an FEI XL30 FEGSEM operated at 5 kV. Images were recorded digitally using XL30 software at 500× magnification.
Darragh K., Vanjari S., Mann F., Gonzalez-Rojas M.F., Morrison C.R., Salazar C., Pardo-Diaz C., Merrill R.M., McMillan W.O., Schulz S, & Jiggins C.D. (2017). Male sex pheromone components in Heliconius butterflies released by the androconia affect female choice. PeerJ, 5, e3953.
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