Osteomeasure xp system

Manufactured by OsteoMetrics

Sourced in United States

The Osteomeasure XP system is a lab equipment product designed for the measurement and analysis of bone density and structure. It provides accurate and reliable data to support research and clinical applications.

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Lab products found in correlation

50i microscope, by Nikon (2 mentions) Trap staining kit 387 a, by Merck Group (1 mentions)

Spelling variants of this product

Osteomeasure (81 citations) OsteoMeasure system (70 citations)

5 protocols using osteomeasure xp system

Bone Histomorphometry Analysis Protocol

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Right femurs were harvested, cleaned, fixed in 70% ethanol, dehydrated in 100% ethanol and embedded in methylmethacrylate. Three micron sections, cut with a Microm HM360 microtome, were then stained with Mason-Goldner trichrome stain for measurement of static and dynamic parameters of bone structure, formation and resorption, as described by others (24 (link)). Measurements of bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), % osteoid volume (OV/BV), % osteoid surface (OS/BS), osteoid thickness (O.Th), osteoblast number/bone length (N.Ob/B.Pm), osteoblast surface/bone surface (Ob.S/BS), erosion surface/bone surface (ES/BS), erosion depth (E.De), osteoclast number/bone length (N.Oc/B.Pm), osteoclast surface/bone surface (Oc.S/BS), mineral apposition rate (MAR), double labels/bone surface (dLS/BS), single labels/bone surface (sLS/BS), mineralization surface/bone surface (MS/BS), bone formation rate/bone surface (BFR/BS), mineralization lag time (MLT) and osteoid maturation time (OMT) were obtained using Osteomeasure XP system (OsteoMetrics, Inc., Decatur, GA).

Thrailkill K.M., Nyman J.S., Bunn R.C., Uppuganti S., Thompson K.L., Lumpkin CK J.r., Kalaitzoglou E, & Fowlkes J.L. (2016). The impact of SGLT2 Inhibitors, compared with Insulin, on Diabetic Bone Disease in a mouse model of Type 1 Diabetes. Bone, 94, 141-151.

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Histomorphometric Analysis of Splenic Microstructure

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Sections (4 μm) of formalin-fixed paraffin-embedded spleens isolated from 4- and 12-week-old eYFP-Osx:Cre, eYFP-Rptor_ob^+/− and eYFP-Rptor_ob^−/− mice (n = 8–10 per group) were stained with haematoxylin and eosin. Histomorphometric analysis of white pulp area, total spleen area and follicle number was performed using the OsteoMeasure XP system (Osteometrics Inc., GA, USA). For quantitative assessment of white pulp area, follicle number and follicle size between mouse cohorts, values were normalised to total spleen area in order to account for the reduced spleen size of eYFP-Rptor_ob^−/− mice.

Martin S.K., Fitter S., El Khawanky N., Grose R.H., Walkley C.R., Purton L.E., Ruegg M.A., Hall M.N., Gronthos S, & Zannettino A.C. (2018). mTORC1 plays an important role in osteoblastic regulation of B-lymphopoiesis. Scientific Reports, 8, 14501.

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Bone Histomorphometric Analysis Protocol

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Right femurs were harvested, cleaned, fixed in ethanol, dehydrated and embedded in methylmethacrylate. Three micron sections, cut with a microtome, were stained with Mason-Goldner trichrome stain for measurement of static and dynamic parameters of bone structure, formation and resorption, as described previously (16 ). Measurements of bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), % osteoid volume (OV/BV), % osteoid surface (OS/BS), osteoid thickness (O.Th), osteoblast number/bone length (N.Ob/B.Pm), osteoblast surface/bone surface (Ob.S/BS), erosion surface/bone surface (ES/BS), erosion depth (E.De), osteoclast number/bone length (N.Oc/B.Pm), osteoclast surface/bone surface (Oc.S/BS), mineral apposition rate (MAR), double labels/bone surface (dLS/BS), single labels/bone surface (sLS/BS), mineralization surface/bone surface (MS/BS), bone formation rate (BFR), mineralization lag time (MLT) and osteoid maturation time (OMT) were obtained using Osteomeasure XP system (OsteoMetrics, Inc., Decatur, GA).

Fowlkes J.L., Bunn R.C., Ray P.D., Kalaitzoglou E., Uppuganti S., Unal M., Nyman J.S, & Thrailkill K.M. (2019). Constitutive activation of MEK1 in osteoprogenitors increases strength of bone despite impairing mineralization. Bone, 130, 115106.

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Dynamic Bone Histomorphometry in Mice

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For dynamic bone histomorphometry, mice were intraperitoneally injected with 30 mg/kg calcein 9 days prior to sacrifice and then with 10 mg/kg Alizarin Red at 2 days prior to sacrifice. Tibia were harvested and embedded with methyl methacrylate. Six-micron sections were cut using a Leica SM2500 metallurgical cutting system. For the measurement of in vivo osteoclast activity, tibiae were harvested from 12 week old mice. TRAP staining was performed on histological sections using a Sigma Trap staining kit (387-A). The fluorescently-labeled and TRAP stained images were photographed using a Nikon 50i microscope. Histomorphometric parameters for double labels and osteoclast activity were assessed using an OsteoMeasurexp system (OsteoMetrics Inc, GA, USA).

Ge C., Zhao G., Li B., Li Y., Cawthorn W.P., MacDougald O.A, & Franceschi R.T. (2017). Genetic Inhibition of PPARγ S112 Phosphorylation Reduces Bone Formation and Stimulates Marrow Adipogenesis. Bone, 107, 1-9.

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Evaluating Mouse Bone Dynamics

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For dynamic bone histomorphometry, 12 week old mice were intraperitoneally injected with 30 mg/kg calcein 9 days prior to sacrifice and then with 10 mg/kg Alizarin Red at 2 days prior to sacrifice. Tibia were harvested and embedded with methyl methacrylate. Six micron sections were cut using Leica SM2500 metallurgical cutting system. For the measurement of in vivo osteoclast activity, tibea were harvested from 12 week old mice. TRAP staining was performed on histological sections using the Sigma Trap staining kit (387-A). The fluorescence and TRAP stained images were photographed using a Nikon 50i microscope. Histomorphometric parameters for double labels and osteoclast activity were assessed using an OsteoMeasurexp system (OsteoMetrics Inc, GA, USA).

Ge C., Wang Z., Zhao G., Li B., Liao J., Sun H, & Franceschi R.T. (2016). Discoidin Receptor 2 Controls Bone Formation and Marrow Adipogenesis. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 31(12), 2193-2203.

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