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Cd73 pe clone ebioty 11

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CD73-PE (clone eBioTY/11.8) is a fluorescently-labeled monoclonal antibody that binds to the CD73 cell surface antigen. CD73 is an enzyme involved in the regulation of extracellular adenosine levels. This antibody can be used to identify and characterize CD73-expressing cells in flow cytometry applications.

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Lab products found in correlation

Cd31 pe cy7 clone 390, by Thermo Fisher Scientific (2 mentions) Cd41 percp efluor710 clone ebiomwreg30, by Thermo Fisher Scientific (2 mentions) Cd45 percp cy5.5 clone 102, by BD (2 mentions) Ter119 eflour450 clone ter 119, by Thermo Fisher Scientific (2 mentions) Cd184 apc clone 2b11, by BD (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions)

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CD73-PE (19 citations)

2 protocols using cd73 pe clone ebioty 11

1

Runx1-GFP Mouse Embryo Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Timed matings of Runx1GFP mice (Runx1tm4Dow)43 (link) were performed to generate embryos, with vaginal plug considered as embryonic day (E) 0.5. Embryos with 32–40 somite pairs were staged as E10.5. AGM and YS were disaggregated with collagenase, as previously described44 (link). The following mouse Abs were used: CD31 PE-Cy7 (clone 390, eBioscience, 1:200), CD41-PerCP-eFluor710 (clone eBioMWReg30, eBioscience, 1:200), CD45-PerCP-Cy5.5 (clone 102, BD Pharmingen, 1:200), Ter119-eFlour450 (clone TER-119, eBioscience, 1:200), CD184-APC (clone 2B11, BD Pharmingen, 1:200), CD73-PE (clone eBioTY/11.8, eBioscience, 1:200). DAPI (Life Technologies) was used to exclude dead cells. No statistical method was used to predetermine sample size. The experiments were not randomized. The investigators were not blinded to allocation during experiments and outcome assessment.
Ditadi A., Sturgeon C.M., Tober J., Awong G., Kennedy M., Phillips A., Azzola L., Ng E.S., Stanley E., French D.L., Cheng X., Gadue P., Speck N., Elefanty A.G, & Keller G. (2015). HUMAN DEFINITIVE HAEMOGENIC ENDOTHELIUM AND ARTERIAL VASCULAR ENDOTHELIUM REPRESENT DISTINCT LINEAGES. Nature cell biology, 17(5), 580-591.
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2

Runx1-GFP Mouse Embryo Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Timed matings of Runx1GFP mice (Runx1tm4Dow)43 (link) were performed to generate embryos, with vaginal plug considered as embryonic day (E) 0.5. Embryos with 32–40 somite pairs were staged as E10.5. AGM and YS were disaggregated with collagenase, as previously described44 (link). The following mouse Abs were used: CD31 PE-Cy7 (clone 390, eBioscience, 1:200), CD41-PerCP-eFluor710 (clone eBioMWReg30, eBioscience, 1:200), CD45-PerCP-Cy5.5 (clone 102, BD Pharmingen, 1:200), Ter119-eFlour450 (clone TER-119, eBioscience, 1:200), CD184-APC (clone 2B11, BD Pharmingen, 1:200), CD73-PE (clone eBioTY/11.8, eBioscience, 1:200). DAPI (Life Technologies) was used to exclude dead cells. No statistical method was used to predetermine sample size. The experiments were not randomized. The investigators were not blinded to allocation during experiments and outcome assessment.
Ditadi A., Sturgeon C.M., Tober J., Awong G., Kennedy M., Phillips A., Azzola L., Ng E.S., Stanley E., French D.L., Cheng X., Gadue P., Speck N., Elefanty A.G, & Keller G. (2015). HUMAN DEFINITIVE HAEMOGENIC ENDOTHELIUM AND ARTERIAL VASCULAR ENDOTHELIUM REPRESENT DISTINCT LINEAGES. Nature cell biology, 17(5), 580-591.
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