Male wistar rats

Male Wistar rats (200–225 g; Charles River Laboratories, Wilmington, MA) were housed 2 per cage at 22 ± 1°C, under a 12 h light-dark cycle with food and water available ad libitum, and habituated to the housing conditions for one week prior to the experiments. Animals were treated sub-chronically (twice a day for 7 days, at approximately 8:00 a.m. and 8:00 p.m.) with either saline (1 ml/kg) or PCP (5 mg/kg) via intraperitoneal route (i.p.) and tested starting 5 days after the last drug injection; no PCP was on board or administered during the behavioral assessment and electrophysiological recordings. Therefore, given an approximate age of 49–52 days upon arrival, the animals were young adults at the time of experimentation. All experiments were carried out in accordance with the National Institute of Health Guide for the Care and Use of Laboratory Animals and approved by the Institutional Animal Care and Use Committee of the University of Texas Health Science Center at San Antonio.
For the experiment “Wistar vs. Sprague Dawley rats”, Male Wistar rats (Charles River Laboratories, Wilmington, MA) were compared to male adult Sprague Dawley rats (Harlan Laboratories, Indianapolis, IN).

Male Wistar rats (Beijing Vital River Laboratory Animal Technology Co., Ltd.) weighing between 200 and 220 g were randomly allocated to the control and anti‐Thy1 groups. All rats were housed in an animal care facility under a light/dark cycle of 12/12 hours with free access to food and water. Welfare‐related assessments and interventions were performed throughout the experiment. In total, 28 rats were injected with a mouse anti‐Thy1 monoclonal antibody, as described previously,¹⁶ and seven were injected with saline (controls). Glomeruli were purified from the renal cortex tissue using a sieving method. Sera were collected for measurements of serum urea nitrogen and creatinine levels. Anti‐Thy1 model rats were killed on days 0, 3, 5, 7 and 10, and their glomeruli were harvested. All animal welfare and experimental procedures were performed in strict accordance with the Guide for the Care and Use of Laboratory Animals (USA National Research Council, 1996).