Log In Sign up
The largest database of trusted experimental protocols

Mosquito liquid handler

Manufactured by SPT Labtech
Visit Supplier

The Mosquito liquid handler is a precision pipetting system designed for accurate and reproducible liquid handling in a laboratory setting. It features an automated pipetting mechanism that can precisely aspirate and dispense liquids with high repeatability. The Mosquito is suitable for a range of liquid handling applications, including sample preparation, assay development, and compound management.

Automatically generated - may contain errors

Lab products found in correlation

Ampure beads, by Thermo Fisher Scientific (5 mentions) Cfx96 touch real time pcr detection system, by Bio-Rad (3 mentions) Nextseq 500 sequencing system, by Illumina (2 mentions) Nextera xt library sample preparation kit, by Illumina (2 mentions) Nextera tagmentation dna buffer, by Illumina (2 mentions) Neutralize tagment buffer, by Illumina (2 mentions) Nextera npm mix, by Illumina (2 mentions) Ampure beads, by SPT Labtech (2 mentions) Tn5 enzyme, by Illumina (2 mentions) Nextseq 500, by Illumina (2 mentions) Novaseq 6000 sequencing system, by Illumina (2 mentions) Pymol molecular graphics system version 1, by Schrödinger (1 mentions) Marvinsketch, by ChemAxon (1 mentions) Micromax 007hf, by Rigaku (1 mentions) Sparse matrix screens, by Molecular Dimensions (1 mentions) Saturn 944 ccd detector, by Rigaku (1 mentions) Multidrop dispenser, by Thermo Fisher Scientific (1 mentions) Sh800, by Sony (1 mentions) 150 cycle high output kit, by Illumina (1 mentions) S4 300 cycle kit, by Illumina (1 mentions)

11 protocols using mosquito liquid handler

1

MERS-CoV Fab Complex Crystallization

Check if the same lab product or an alternative is used in the 5 most similar protocols
All crystallization steps were conducted at 18 °C. Any precipitated material of MERS-CoV complexed with KNIH90-F1 Fab was removed by centrifugation prior to the initial screening trials. Initial crystallization trials of the complex using commercial screens were carried out using mosquito liquid handler (TTP Labtech). The ideal crystals were grown in a buffer containing 0.1 M MES monohydrate at pH 6.3 and 15% (w/v) polyethylene glycol 20,000. The size up of crystals was carried out using hanging-drop vapor diffusion in which drops containing 1 μl of protein and 1 μl of mother liquor were equilibrated above a 400-μl mother liquor reservoir. The crystals were soaked in 20% (v/v) ethylene glycol for cryopreservation and quickly frozen in liquid nitrogen on a mount prior to X-ray diffraction.
Jang T.H., Park W.J., Lee H., Woo H.M., Lee S.Y., Kim K.C., Kim S.S., Hong E., Song J, & Lee J.Y. (2022). The structure of a novel antibody against the spike protein inhibits Middle East respiratory syndrome coronavirus infections. Scientific Reports, 12, 1260.
+ Open protocol
+ Expand
2

Structural Determination of SCRIB PDZ4

Check if the same lab product or an alternative is used in the 5 most similar protocols
SCRIB PDZ4 R1110G was concentrated to 11 mg/mL in 20 mM Tris at pH 8.0, 200 mM NaCl, and 5 mM DTT and screened against crystallization conditions using a Mosquito Liquid Handler (TTP Labtech, Cambridge, MA). Final crystals were obtained in 21% PEG 3,350 and 0.25 M Ammonium Nitrate. Crystals were flash frozen in mother liquor supplemented with 15% glycerol. All diffraction data was collected at the Advanced Light Source at Berkeley on beam line 8.2.1, integrated with XDS58 (link), and scaled with AIMLESS59 (link),60 (link). Phases were determined by molecular replacement using Phaser61 and SCRIB PDZ4 (39; PDB ID: 4WYT) as a search model. The Phaser solution was manually rebuilt over multiple cycles using Coot62 (link) and refined using PHENIX63 . All images were generated using the PyMOL Molecular Graphics System, Version 1.74 Schrödinger, LLC. Coordinate files have been deposited in the Protein Data Bank under the accession code 6EEY.
Janezic E.M., Harris D.A., Dinh D., Lee K.S., Stewart A., Hinds T.R., Hsu P.L., Zheng N, & Hague C. (2019). Scribble co-operatively binds multiple α1D-adrenergic receptor C-terminal PDZ ligands. Scientific Reports, 9, 14073.
+ Open protocol
+ Expand
3

BPTF Protein Co-Crystallization Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Crystallization screening campaigns were performed at 18 °C with precipitant solutions from Hampton Research using a Mosquito liquid handler (TTP Labtech). Robust crystallization conditions were established using 25% PEG 3,350 (w/v), 0.2 M lithium sulfate monohydrate, 0.1 M Bis-Tris (pH 6.5) mixed with an equal volume of protein in hanging droplets (2.5 mg/ml final concentration of BPTF). Inhibitors were cocrystallized with BPTF at 1 mM final concentration. Crystals were cryoprotected by addition of 20% ethylene glycol in the precipitant and flash-frozen in liquid N2. During data collection, crystals were maintained under a constant stream of N2 gas (−180 ° x-ray diffraction data were recorded at beamlines 22-ID, 22-BM, 21-ID-D and GM/CA of Argonne National Laboratories. Data were indexed and scaled with XDS32 (link). Phasing and refinement was performed using PHENIX33 (link) and model building with Coot34 (link). PDB entry 3UV2 served as the search model for molecular replacement. Initial models for small molecule ligands were generated through MarvinSketch (ChemAxon, Cambridge, MA) and ligands restraints through eLBOW of the PHENIX suite. All structures have been validated by MolProbity. Figures were prepared using PyMOL (Schrödinger, LLC).
Ycas P.D., Zahid H., Chan A., Olson N.M., Johnson J.A., Talluri S.K., Schonbrunn E, & Pomerantz W.C. (2020). New Inhibitors for the BPTF Bromodomain Enabled by Structural Biology and Biophysical Assay Development. Organic & biomolecular chemistry, 18(27), 5174-5182.
+ Open protocol
+ Expand
4

Crystallization of Aspartate Synthase from Mycobacterium smegmatis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Prior to setting up crystallisation experiments, protein was dialysed into 20 mM Tris-HCl pH 8.0, 50 mM NaCl, 10% glycerol, 100 µM EDTA and 1 mM DTT and concentrated by ultrafiltration. Crystals of Ms-AspS were obtained by vapour diffusion in 96-well sitting drop plates (SwissSci), using a Mosquito liquid handler (TTP Labtech) to pipette droplets of 100 nl Ms-AspS (25 mg/ml) +100 nl reservoir solution. Initial crystals were obtained from commercial sparse matrix screens (Molecular Dimensions) grown over a reservoir of 30% (v/v) pentaerythritol ethoxylate, 0.1 M magnesium formate, 0.1 M Tris-HCl, pH 8.5. Crystals were briefly immersed in reservoir solution complemented with 15% glycerol for cryoprotection, mounted in nylon loops and flash frozen in liquid nitrogen. X-ray diffraction data was recorded on a MicroMax 007HF X-ray generator equipped with a Saturn 944 CCD detector (Rigaku), integrated and scaled using XDS, XSCALE [46] (link). Initial phases were obtained by molecular replacement (PHASER, search model pdb entry 1C0A) [47] (link). The model was rebuilt and refined [48] (link), [49] (link). Coordinates and structure factors for Ms-AspS are deposited in the Protein Data Bank (www.rcsb.org).
Gurcha S.S., Usha V., Cox J.A., Fütterer K., Abrahams K.A., Bhatt A., Alderwick L.J., Reynolds R.C., Loman N.J., Nataraj V., Alemparte C., Barros D., Lloyd A.J., Ballell L., Hobrath J.V, & Besra G.S. (2014). Biochemical and Structural Characterization of Mycobacterial Aspartyl-tRNA Synthetase AspS, a Promising TB Drug Target. PLoS ONE, 9(11), e113568.
+ Open protocol
+ Expand
5

High-Throughput SIRPα Binding Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
The assay was conducted as in Table 1, and all manipulation/incubations were subsequently carried out at room temperature. Optimized reagent amounts for 384 and 1536 well plates are as in Table 2. DRAQ5-labeled cells were added to 384 or 1536 well plates using a multichannel pipet or a Multidrop dispenser (ThermoFisher). Competing ligand/antibody or control mixed with SIRPα-biotin were added to the cells using a multichannel pipet or Mosquito liquid handler (TTP Labtech). Following NAV488 addition and incubation, cell plates were imaged using the Mirrorball LSC according to Table 1. For measurements involving fixed cells, the cells were fixed in 4% paraformaldehyde for 30 min on ice, washed and then stained as above.
Burgess T.L., Amason J.D., Rubin J.S., Duveau D.Y., Lamy L., Roberts D.D., Farrell C.L., Inglese J., Thomas C.J, & Miller T.W. (2020). A homogeneous SIRPα-CD47 cell-based, ligand-binding assay: Utility for small molecule drug development in immuno-oncology. PLoS ONE, 15(4), e0226661.
+ Open protocol
+ Expand
6

Single-cell RNA-seq of tdTomato+ cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysis plates were prepared by dispensing 4 μl lysis buffer as described in Schaum et al. (2018) (link). After dissociation, single tdTomato+ cells were sorted in 96-well plates using SH800S (Sony). Immediately after sorting, plates were sealed with a pre-labelled aluminum seal, centrifuged, and flash frozen on dry ice. cDNA synthesis and library preparation were performed using the Smart-seq2 protocol (Picelli et al., 2014 (link)). Wells of each library plate were pooled using a Mosquito liquid handler (TTP Labtech). Pooling was followed by two purifications using 0.7x AMPure beads (Fisher, A63881). Library quality was assessed using capillary electrophoresis on a Fragment Analyzer (AATI), and libraries were quantified by qPCR (Kapa Biosystems, KK4923) on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Libraries were sequenced on the NextSeq 500 Sequencing System (Illumina) using 2 × 75 bp paired-end reads and 2 × 8 bp index reads.
Ren J., Isakova A., Friedmann D., Zeng J., Grutzner S.M., Pun A., Zhao G.Q., Kolluru S.S., Wang R., Lin R., Li P., Li A., Raymond J.L., Luo Q., Luo M., Quake S.R, & Luo L. (2019). Single-cell transcriptomes and whole-brain projections of serotonin neurons in the mouse dorsal and median raphe nuclei. eLife, 8, e49424.
+ Open protocol
+ Expand
7

Single-cell RNA-seq library prep using SmartSeq2

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
cDNA synthesis was performed using the plate-based SmartSeq2 protocol41 (link), with 20 cycles of amplification during the PCR step. Amplified cDNA was purified twice with 0.7x AMPure beads (Fisher A63881). cDNA quality and concentration were then assessed by capillary electrophoresis on a Fragment analyzer (AATI) before sequencing library preparation. Illumina sequencing libraries for cDNA from single cells were prepared as previously described41 (link). Briefly, cDNA libraries were prepared using the Nextera XT Library Sample Preparation kit (Illumina, FC-131–1096). Nextera tagmentation DNA buffer (Illumina) and Tn5 enzyme (Illumina) were added, and the sample was incubated at 55°C for 10 minutes. The reaction was neutralized by adding “Neutralize Tagment Buffer” (Illumina) and centrifuging at room temperature at 3,220 × g for 5 minutes. Samples were then indexed via PCR by adding i5 indexing primer, i7 indexing primer, and Nextera NPM mix (Illumina). Following cDNA sequencing library preparation, wells of each library plate were pooled using a Mosquito liquid handler (TTP Labtech), then purified twice using 0.7x AMPure beads. Libraries were sequenced on a NextSeq 500 (Illumina) using 75bp paired-end sequencing.
Veerakumar A., Yung A.R., Liu Y, & Krasnow M.A. (2022). Molecularly defined circuits for cardiovascular and cardiopulmonary control. Nature, 606(7915), 739-746.
+ Open protocol
+ Expand
8

Pooling and Sequencing of Thymus Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following library preparation, wells of each library plate were pooled using a Mosquito liquid handler (TTP Labtech). Row A of the thymus plates, which contained bulk cells, was pooled separately. Pooling was followed by two purifications using 0.7x AMPure beads (Fisher, A63881). Library quality was assessed using capillary electrophoresis on a Fragment Analyzer (AATI), and libraries were quantified by qPCR (Kapa Biosystems, KK4923) on a CFX96 Touch Real-Time PCR Detection System (Biorad). Plate pools were normalised to 2 nM and sequenced on the NovaSeq 6000 Sequencing System (Illumina) using 2 × 100 bp paired-end reads with an S4 300 cycle kit (Illumina, 20012866). Row A thymus pools were normalised to 2 nM and sequenced separately on the NextSeq 500 Sequencing System (Illumina) using 2 × 75 bp paired-end reads with a High Output 150 cycle kit (Illumina, FC-404–2002).
Baran-Gale J., Morgan M.D., Maio S., Dhalla F., Calvo-Asensio I., Deadman M.E., Handel A.E., Maynard A., Chen S., Green F., Sit R.V., Neff N.F., Darmanis S., Tan W., May A.P., Marioni J.C., Ponting C.P, & Holländer G.A. (2020). Ageing compromises mouse thymus function and remodels epithelial cell differentiation. eLife, 9, e56221.
+ Open protocol
+ Expand
9

Single-cell RNA-seq library prep using SmartSeq2

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
cDNA synthesis was performed using the plate-based SmartSeq2 protocol41 (link), with 20 cycles of amplification during the PCR step. Amplified cDNA was purified twice with 0.7x AMPure beads (Fisher A63881). cDNA quality and concentration were then assessed by capillary electrophoresis on a Fragment analyzer (AATI) before sequencing library preparation. Illumina sequencing libraries for cDNA from single cells were prepared as previously described41 (link). Briefly, cDNA libraries were prepared using the Nextera XT Library Sample Preparation kit (Illumina, FC-131–1096). Nextera tagmentation DNA buffer (Illumina) and Tn5 enzyme (Illumina) were added, and the sample was incubated at 55°C for 10 minutes. The reaction was neutralized by adding “Neutralize Tagment Buffer” (Illumina) and centrifuging at room temperature at 3,220 × g for 5 minutes. Samples were then indexed via PCR by adding i5 indexing primer, i7 indexing primer, and Nextera NPM mix (Illumina). Following cDNA sequencing library preparation, wells of each library plate were pooled using a Mosquito liquid handler (TTP Labtech), then purified twice using 0.7x AMPure beads. Libraries were sequenced on a NextSeq 500 (Illumina) using 75bp paired-end sequencing.
Veerakumar A., Yung A.R., Liu Y, & Krasnow M.A. (2022). Molecularly defined circuits for cardiovascular and cardiopulmonary control. Nature, 606(7915), 739-746.
+ Open protocol
+ Expand
10

Bulk RNA-seq Library Preparation and Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following library preparation, wells of each library plate were pooled using a Mosquito liquid handler (TTP Labtech). Pooling was followed by two purifications using ×0.7 AMPure beads (Fisher, A63881). Library quality was assessed using capillary electrophoresis on a Fragment Analyzer (AATI), and libraries were quantified by qPCR (Kapa Biosystems, KK4923) on a CFX96 Touch Real-Time PCR Detection System (Biorad). Plate pools were normalized to 2 nM and equal volumes from 10 or 20 plates were mixed together to make the sequencing sample pool. PhiX control library was spiked in at 0.2% before sequencing. Single-cell libraries were sequenced on the NovaSeq 6000 Sequencing System (Illumina) using 2 × 100 bp paired-end reads and 2 × 8 bp or 2 × 12 bp index reads with a 300-cycle kit (Illumina 20012860).
Reinitz F., Chen E.Y., Nicolis di Robilant B., Chuluun B., Antony J., Jones R.C., Gubbi N., Lee K., Ho W.H., Kolluru S.S., Qian D., Adorno M., Piltti K., Anderson A., Monje M., Heller H.C., Quake S.R, & Clarke M.F. (2022). Inhibiting USP16 rescues stem cell aging and memory in an Alzheimer’s model. eLife, 11, e66037.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!