Coumarin

Manufactured by Thermo Fisher Scientific

Sourced in United States

Coumarin is a chemical compound used in various laboratory applications. It functions as a fluorescent dye, with the ability to emit light when exposed to ultraviolet or blue light. This property makes Coumarin a valuable tool for researchers and scientists in a range of fields, including biochemistry, cell biology, and analytical chemistry.

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Lab products found in correlation

Spelling variants of this product

Coumarin-6 (9 citations) 7-ethoxy-4-(trifluoromethyl)coumarin (3 citations) 7-ethoxy-4-trifluoromethyl coumarin (7-EFC) (2 citations)

5 protocols using coumarin

Investigating NF-κB Activation in THP-1 Cells

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The CellSensor NFκB-bla human monocytic THP-1 cell line was purchased from Thermo Fisher Scientific (Waltham, MA). This cell line contains a stably integrated beta-lactamase reporter gene under the control of the nuclear factor kappa B (NF-κB) response element (https://tools.thermofisher.com/content/sfs/manuals/CellSensor_NFkBbla_THP1_man.pdf). NF-κB activation results in beta-lactamase production, which shifts the fluorescence emission of the beta-lactamase substrate (LiveBLAzerTM-FRET B/G (CCF4-AM), Thermofisher) to favor coumarin (460 nm emission) over fluorescein (530 nm emission). Murine or human TLR4 HEK Blue cells were purchased from Invivogen (San Diego, CA). These cell lines were stably cotransfected with human or mouse TLR4, MD-2, and CD14 coreceptor genes and an inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene. QuantiBlue was purchased from Invivogen. MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-dipheyl tetrazolium bromide) was purchased from Acros Organics, and ovalbumin was purchased from Worthington Biochemical Corporation. For the HTS, LPS E. coli 0111:B4 (Sigma-Aldrich) was used, and LPS-EB Ultrapure (cat# tlrl-3pelps, Invivogen) was used in the validation and subsequent SAR studies. Wortmannin,^{33 (link)} a nonspecific, covalent inhibitor of PI3K, and MPLA were purchased from Invivogen.

Chan M., Ahmadi A., Yao S., Sato-Kaneko F., Messer K., Pu M., Nguyen B., Hayashi T., Corr M., Carson D.A., Cottam H.B, & Shukla N.M. (2017). Identification of Biologically Active Pyrimido[5,4-b]indoles That Prolong NF-κB Activation without Intrinsic Activity. ACS combinatorial science, 19(8), 533-543.

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Purification and Labeling of Dbp5 and P_i_BiP

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N-terminal 6xHis tagged Dbp5 was expressed and purified from Escherichia coli as previously described ^{81 (link)}. The E. coli phosphate binding protein (P_iBiP) was purified and labeled with MDCC (7-diethylamino-3-(((2-maleimidyl)ethyl)amino)carbonyl) coumarin; Thermo Fisher Scientific Inc.) as described.^{44 (link), 82 (link)}

Wong E.V., Cao W., Vörös J., Merchant M., Modis Y., Hackney D.D., Montpetit B, & De La Cruz E.M. (2016). Pi Release Limits the Intrinsic and RNA-Stimulated ATPase Cycles of DEAD-Box Protein 5 (Dbp5). Journal of Molecular Biology, 428(2Part B), 492-508.

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Endocytosis Inhibition in Cancer Cells

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All cell lines (LNCaP, DU145, PC3, WPE1-NA22) were purchased from American Type Culture Collection (ATCC) (Manassas, VA, USA). MD was purchased from Grain Processing Corporation (Muscatine, IA, USA). PCL (MW =80,000 g/mol) and methylene chloride, polyvinyl alcohol, methyl-β-cyclodextrin (MβCD), and nystatin were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Amiloride was purchased from Tocris Bioscience (Bristol, UK), chlorpromazine (CPZ) was purchased from Enzo Life Sciences (Farmingdale, NY, USA), and coumarin was purchased from Acros Organics (Thermo Fisher Scientific). RPMI-1640 medium, F-12 medium, penicillin–streptomycin, fetal bovine serum, and LysoTracker Red™ were purchased from Gibco^® Invitrogen Corporation (Grand Island, NY, USA).

Korang-Yeboah M., Gorantla Y., Paulos S.A., Sharma P., Chaudhary J, & Palaniappan R. (2015). Polycaprolactone/maltodextrin nanocarrier for intracellular drug delivery: formulation, uptake mechanism, internalization kinetics, and subcellular localization. International Journal of Nanomedicine, 10, 4763-4781.

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Extraction and Analysis of Vanillin and Coumarin

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Vanillin and coumarin were purchased from ACROS Organics (New Jersey, USA). HPLC grade methanol and phenol were obtained from Fischer Scientific (Fair Lawn, NJ, USA). Acetone was acquired from VWR International (West Chester, PA, USA). The 18 MΩ-cm deionized water was prepared in the laboratory using a PureLab ultra MK2 system from ELGA (Lane End, Buckinghamshire, England). Adsorbosil C18 (4.6 × 150 mm, 5 µm) was bought from Alltech Associates, Inc. (Deerfield, IL, USA). Stainless steel vessels (7.07 mL, 9 cm × 1 cm ID) were purchased from Raleigh Valve and Fitting Company (Raleigh, NC, USA). Caffeine was acquired from Eastman Kodak Co. (Rochester, NY, USA). Vanilla beans were purchased from Florida Herb House (Daytona, FL, USA), while whole tonka beans were bought from Amazon.

Doctor N., Parker G., Vang K., Smith M., Kayan B, & Yang Y. (2020). Stability and Extraction of Vanillin and Coumarin under Subcritical Water Conditions. Molecules, 25(5), 1061.

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Polyester Dyeing with Novel Eco-Friendly Carriers

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The fabric used was a PES 100% woven standard polyester, Type 30 A, from Testgewebe GmbH (Brüggen, Germany) (ISO 105-F10). The high molecular weight disperse dyes used were Rubi Foron S-2GFL (C.I Disperse Red 167) and Navy Blue Foron S-BRL (Disperse Blue 79). All dyes were supplied by Clariant (Prattlen, Switzerland). Coumarin (99% purity) was supplied by Acros (NJ, USA) and o-vanillin (99% purity) was supplied by Acros (NJ, USA). Both carriers use agro-sourced alternative compounds with additional properties, such as antimicrobial, antioxidant, biodegradability and antimutagenic. Also, these carriers are hydrophobic with no toxicity, and we expect them to have better influence on polyester dyeing [10 (link),16 (link)]. The chemical structure of these chemicals is presented in Table 1.
Pure n-butyl acetate (M_w = 116.16 g·mol^–1), supplied by Panreac (Barcelona, Spain), was used as the co-solvent. N,N-Dimethylformamide (M_w = 73.10 g·mol^–1), 99.8% purity and supplied by Panreac (Barcelona, Spain), was used as the solvent to extract the dyes from the PES fabrics. Hostapal non-ionic surfactant detergent, supplied by Archroma GmbH (Sulzbach, Germany), was used as for pre-dyeing washing. QP-grade 85% pure sodium dithionite (M_w = 174.11 g·mol^–1) and analytical-grade sodium hydroxide, both supplied by Panreac, were used as a post-dyeing washing reductant.

Radei S., Carrión-Fité F.J., Ardanuy M, & Canal J.M. (2018). Kinetics of Low Temperature Polyester Dyeing with High Molecular Weight Disperse Dyes by Solvent Microemulsion and AgroSourced Auxiliaries. Polymers, 10(2), 200.

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