Eight-week-old male C57BL/6 mice were randomly divided into 3 groups (9 animals for each group) as follows: the CON group, mice were fed normal drinking water; the DSS group, mice were fed 3% DSS in drinking water from Day 6 to Day 11) as the IBD model (15 (link)); and the DSS + IPA group, IPA(Sigma, CAS 830-96-6);at doses of 200 mg/kg body weight/day dissolving in 0.5% arboxymethyl cellulose sodium salt (CMC) was administered to mice by oral gavage and 3% DSS in the drinking water from Day 6 to Day 11. All mice were given free access to food and drinking water. On Day 12, the mice were euthanized, and samples were collected for subsequent analysis.
Ingenuity pathway analysis (ipa)
Manufactured by Merck Group
Sourced in United States, Germany
IPA is a laboratory instrument used for the analysis and purification of various chemical compounds. It provides precise control over the temperature and pressure conditions during the separation and identification of substances. IPA is a core component in many analytical and research workflows across a range of industries.
Automatically generated - may contain errors
Lab products found in correlation
Ethanol, by Merck Group (7 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (7 mentions)
Methanol, by Merck Group (5 mentions)
N n dimethylformamide (dmf), by Merck Group (4 mentions)
Acetone, by Merck Group (3 mentions)
2 butanol, by Merck Group (3 mentions)
1 butanol, by Merck Group (3 mentions)
Tween 20, by Merck Group (3 mentions)
Cis diammineplatinum 2 dichloride, by Merck Group (2 mentions)
Pluronic f 127, by Merck Group (2 mentions)
Su 8 developer, by MicroChem (2 mentions)
Neomycin sulfate, by Merck Group (2 mentions)
Vancomycin, by Merck Group (2 mentions)
Metronidazole, by Merck Group (2 mentions)
Indole, by Merck Group (2 mentions)
Ch223191, by Merck Group (2 mentions)
Specific primary antibodies of ahr and cyp1a1, by Affinity Biosciences (2 mentions)
Phosphorylation p of p65 and iκb, by Cell Signaling Technology (2 mentions)
Occludin, by Bioss Antibodies (2 mentions)
Claudin 3, by Bioss Antibodies (2 mentions)
68 protocols using ingenuity pathway analysis (ipa)
1
Investigating IPA's Effects on DSS-Induced IBD
2
Intranasal Infection of Mice with H3N2 Influenza and IPA Treatment
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For infection, mice were anesthetized by intramuscular administration of 1.25 mg of ketamine and 0.25 mg of xylazine in 100 µl of phosphate buffered saline (PBS), and then intranasally (i.n.) infected with 50 μl of PBS containing 50 pfu of the H3N2 IAV strain A/Scotland/20/1974.71 (link),72 (link) Mice treated i. n. with PBS served as controls (non-infected mice). To study the impact of IPA treatment, mice were supplemented daily with IPA (Sigma-Aldrich, 40 mg/kg/day) or vehicle (0.5% methyl cellulose in sterile water) by oral gavage one day after infection with IAV and until D6 (sacrifice at D7 post-infection) or until D10 (sacrifice at D16 post-infection). The dose of IPA was chosen based on previous publications.64 (link),73 (link) Alternatively, mice were treated by oral gavage with IPA or vehicle one day before IAV infection until D3 (sacrifice at D4 post-infection). Each gavage dose volume was 200 μl. Body weight was monitored daily after IAV infection. To measure body temperature, mice were implanted subcutaneously with TAM-MT radio transmitters capable of monitoring body temperature through receivers (ReadStation, Intellebio innovation, Seichamps, France). Blood samples were collected at sacrifice, and cytokine levels were determined by enzyme-linked immunosorbent assay (ELISA) using kits provided by Invitrogen (Waltham, MA) and MyBioSource (San Diego, CA).
3
Electrospun Polymer Nanofibers with Metallic Coatings
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AgNWs, with an average length and a diameter of 10 μm and 20 nm, respectively, dispersed at 0.15-wt% in isopropyl alcohol (IPA) comprised a precursor obtained from Aiden (Republic of Korea). The precursor was then diluted by adding IPA (Duksan, Republic of Korea) at a weight ratio (IPA/precursor) of 3:1.
Polymer nanofibers (NFs) were fabricated by electrospinning 8 wt% polyacrylonitrile (PAN, Mw = 150 kDa, Sigma-Aldrich, USA) solution dissolved in N,N-dimethylformamide (DMF, 99.8%, Sigma-Aldrich, USA). Copper and nickel electroplating solutions were prepared as follows. The copper-plating solution was prepared by blending 13 g of copper sulfate (Sigma-Aldrich, USA) in 100 ml of deionized water, and then adding 5 g of sulfuric acid (Matsunoen Chemicals, South Korea), 0.5 g of hydrochloric acid (Sigma-Aldrich, USA), and 10 g of formaldehyde (Sigma-Aldrich, USA) under agitation for 3 h at 35 °C on a hotplate. The nickel-plating solution was prepared by dissolving 80 g of Ni(SO3NH2)2•4H2O (Sigma-Aldrich, USA) and 6 g of H3BO3 (Sigma-Aldrich, USA) in 200 mL of deionized water under stirring for 3 h at 35 °C on the hotplate. Prior to use of the solutions, a specific amount of 1 M sodium hydroxide (NaOH, Sigma-Aldrich, USA) was added to adjust the pH of the electroplating solutions at 4.5.
Polymer nanofibers (NFs) were fabricated by electrospinning 8 wt% polyacrylonitrile (PAN, Mw = 150 kDa, Sigma-Aldrich, USA) solution dissolved in N,N-dimethylformamide (DMF, 99.8%, Sigma-Aldrich, USA). Copper and nickel electroplating solutions were prepared as follows. The copper-plating solution was prepared by blending 13 g of copper sulfate (Sigma-Aldrich, USA) in 100 ml of deionized water, and then adding 5 g of sulfuric acid (Matsunoen Chemicals, South Korea), 0.5 g of hydrochloric acid (Sigma-Aldrich, USA), and 10 g of formaldehyde (Sigma-Aldrich, USA) under agitation for 3 h at 35 °C on a hotplate. The nickel-plating solution was prepared by dissolving 80 g of Ni(SO3NH2)2•4H2O (Sigma-Aldrich, USA) and 6 g of H3BO3 (Sigma-Aldrich, USA) in 200 mL of deionized water under stirring for 3 h at 35 °C on the hotplate. Prior to use of the solutions, a specific amount of 1 M sodium hydroxide (NaOH, Sigma-Aldrich, USA) was added to adjust the pH of the electroplating solutions at 4.5.
4
Multicomponent Cell Culture Protocol
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Acetone, ethanol, and IPA were purchased from Millipore. The SU-8 and SU-8 developers were purchased from MicroChem. The amorphous fluoroplastic solution was purchased from Chemours Company. Pluronic F127 was purchased from Sigma-Aldrich (Oakville, ON, USA). Silicone oil (1 Cst) was purchased from Clearco, USA. Fetal bovine serum (FBS), PBS, collagenase II, Hank’s Balanced Salt Solution (HBSS), Earle’s balanced salt solution (EBSS), DMEM/F12, Glutamax, HEPES, and 1:50 B27 were purchased from Gibco. Cis-diammineplatinum (II) dichloride, EP, Y27632, dexamethasone, penicillin/streptomycin, N-acetyl-l-cysteine, nicotinamide, insulin, hydrocortisone, cholera toxin, and hyaluronidase were purchased from Sigma. Wzb117 was purchased from Selleckchem. Recombinant human EGF, recombinant human FGF10, and recombinant human HGF were purchased from Peprotech. RBC lysis buffer, EthD-1, erythrocyte lysate, and Cell Tracker™ Green CMFDA Dye were purchased from Invitrogen. StemMACS iPS-Brew XF medium was purchased from Miltenyl Biotec (USA). Dimethyl sulfoxide, Sor, Reg, Apa, Len, and DNase I were purchased from Solarbio. Forskolin and A8301 were purchased from Tocris.
5
Fabrication of Microfluidic Devices
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IPA, acetone and ethanol were purchased from Millipore. The reagents used for photolithography, including SU-8 and SU-8 developer, were purchased from MicroChem. Amorphous Fluoroplastics Solution was purchased from the Chemours Company. Pluronic F127 was purchased from Sigma Aldrich (Oakville, ON, USA). Silicone oil (1 cSt) was purchased from Clearco, USA. MDA-MB-231 cells and MCF-10A cells were obtained from the American Type Culture Collection (Manassas, VA, USA). Dulbecco’s Modified Eagle’s Medium, fetal bovine serum (FBS), trypsin-EDTA and phosphate buffer solution (PBS) were purchased from Gibco. cis-Diammineplatinum(II) dichloride was purchased from Sigma. Ethidium Homodimer-1 (EthD-1) was purchased from Thermo Fisher Scientific.
6
Gut Microbiome Modulation Protocol
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The main regents used in this study, Trp, indole, IPA, IAld, Ficz, CH223191, metronidazole, ampicillin, vancomycin, and neomycin sulfate, were purchased from Sigma-Aldrich (St. Louis, MO, USA). The specific primary antibodies of AhR and Cyp1a1 were bought from Affinity Biosciences (OH, USA). Phosphorylation (p-) of p65 and IκB, p65, IκB, and β-actin were obtained from Cell Signaling Technology (CST; Boston, USA). The occludin and claudin-3 were bought from Bioss (Beijing, China). MPO activity assay kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). TNF-α and IL-1β enzyme linked immunosorbent assay (ELISA) kits were bought from Biolegend, Inc. (San Diego, CA, USA). indole and IAld ELISA kits were bought from Hnybio (Shanghai, China), and the IPA ELISA kit was obtained from Shyqbio (Shanghai, China). L. reuteri CNCM I-5022 was obtained from the Collection Nationale de Cultures de Microorganisms (CNCM) of the Institut Pasteur. E.coli CVCC1418 was obtained from the China Veterinary Culture Collection Center (CVCC).
7
Preparing a-KB Catalyst Ink
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5 mg of a-KB catalyst was mixed with 1 mL of 2-propanol (IPA, Sigma Aldrich, 99.5%) and 50 μL of 5 wt% Nafion. Then the mixture was sonicated for 10 min. The catalyst ink was sprayed on the carbon paper (TGP-H-120 20%WP, Toray) (11.25 cm2). The mass loading of a-KB on the carbon paper was 1 mg cm−2.
8
L-NAME and Salt-Induced Hypertension in Mice
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At 10 weeks of age, the mice were assigned to one of four treatment groups: two LSHTN groups and two control groups (n = 6 per group). Mice assigned to the LNAME + SALT group were given LSHTN as described above, whereas mice assigned to the LNAME + SALT + IPA group received 562.5 mg/kg body weight IPA (Sigma) added to their 4% salt chow during the diet phase. Control groups were generated to control for diet (LNAME + CON) and IPA treatment (LNAME + CON + IPA). LNAME + CON mice underwent L-NAME priming and a washout period but received regular chow during the diet phase. LNAME + CON + IPA mice also underwent L-NAME priming and a washout period as described above but received regular chow with 562.5 mg/kg IPA during the diet phase. Over the course of treatments, food and water intake were monitored for each cage. These data were averaged to each mouse and can be viewed in Supplemental Figure S1 .
9
Dietary Sugar Modulation of Drosophila Development
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Cultures were carried out on a modified Bloomington semi-defined medium (https://bdsc.indiana.edu/information/recipes/germanfood.html ), containing Sucrose (0.15 and 1.0 M in CD and HSD, respectively) as the only purified sugar source. Ingredients were obtained from; Agar (Fisher Scientific; BP2641-1), Brewer’s Yeast (MP Biomedicals; 903312, Lot: BCBN0171V), Yeast Extract (Sigma-Aldrich; 70161), Peptone (Sigma-Aldrich; 82303), Sucrose (Fisher Scientific; S/8560/63), Magnesium sulfate hexahydrate (Fluka; 00627), Calcium chloride dihydrate (Sigma-Aldrich; 223506), Propionic acid (Sigma-Aldrich; P1386), p-Hydroxy-benzoic acid methyl ester (Sigma-Aldrich; H5501). For dietary supplementation experiments, L-proline (Sigma-Aldrich; P5607), D-proline (Sigma-Aldrich; 858919), L-glycine (VWR Chemicals; 101196X), L-alanine (Sigma-Aldrich; 7627), L-tryptophan (Sigma-Aldrich; T0254), Indole-5-carboxylic acid (Sigma-Aldrich; I5400) and IPA (Sigma-Aldrich; 57400) were added to CD or HSD. Cultures were performed at 25 °C unless otherwise noted. HSD feeding led to a developmental delay in reaching the third-instar larval stage; all experiments were performed on late third-instar larvae at day 8 after egg laying (CD) and day 14 after egg laying (HSD) unless otherwise noted.
10
Metabolite Identification in Biological Samples
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