B16-OVA melanoma cells were kind gift from Dr. Amit Awasthi (TSHTI, India) and were cultured in complete Roswell Park Memorial Institute (RPMI) 1640 medium (AT162, Himedia, India) with 10% heat-inactivated fetal bovine serum (FBS; RM1112, Himedia, India), 100 U/ml penicillin and 100 μg/ml streptomycin (A002, Himedia, India) and 10 mmol/L 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; MB016, Himedia, India). The LentiX lentiviral packaging cell line were kind gift from Dr. Rahul Purwar (IITB, India) and were cultured in Dulbecco’s modified Eagle’s medium (DMEM; AT007, Himedia, India) containing 10% FBS, 2 mmol/L glutamate (TCL030, Himedia, India), 1% non-essential amino acid (ACL006, Himedia, India), 100 U/ml penicillin and 100 μg/ml streptomycin (Himedia, India). Mice splenic T-cells were cultured in complete RPMI 1640 medium (Himedia, India) with 10% heat-inactivated FBS (Himedia, India), 2 mmol/L glutamate (Himedia, India), 1% non-essential amino acid (Himedia, India), 50 U/ml recombinant human interleukin-2 (rhIL-2; 200-02, Peprotech, USA), 100 U/ml penicillin and 100 μg/ml streptomycin (Himedia, India).
Penicillin
Manufactured by HiMedia
Sourced in India, United States, Italy, Germany
Penicillin is a class of antibiotics derived from the Penicillium fungi. It functions as an antimicrobial agent, inhibiting the growth and reproduction of certain bacteria.
Automatically generated - may contain errors
Lab products found in correlation
Streptomycin, by HiMedia (82 mentions)
Fetal bovine serum (fbs), by HiMedia (25 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (22 mentions)
Dulbecco modified eagle medium (dmem), by HiMedia (10 mentions)
L glutamine, by HiMedia (9 mentions)
Erythromycin, by HiMedia (8 mentions)
Ciprofloxacin, by HiMedia (8 mentions)
Tetracycline, by HiMedia (8 mentions)
Amphotericin b, by HiMedia (8 mentions)
Rpmi 1640, by HiMedia (7 mentions)
Ampicillin, by HiMedia (7 mentions)
Trypsin, by HiMedia (6 mentions)
Gentamycin, by HiMedia (5 mentions)
Phosphate buffered saline (pbs), by HiMedia (5 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions)
Linezolid, by HiMedia (4 mentions)
Chloramphenicol, by HiMedia (4 mentions)
Clindamycin, by HiMedia (4 mentions)
Gentamicin, by HiMedia (4 mentions)
Vancomycin, by HiMedia (4 mentions)
111 protocols using penicillin
1
Culturing B16-OVA Melanoma and T-Cells
2
Gfp Tagging of Native B. thuringiensis Strain
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Native B. thuringiensis strain VKK-BB2 (GenBank accession number KT714045) was retrieved from the bacterial stock of Insect Physiology and Molecular Biology Laboratory, Division of Entomology, IARI, New Delhi which was originally isolated from the cabbage aphid, Brevicoryne brassicae (Mandla et al. 2017) . The BtVKK-BB2 strain was found to be the most potential out of 12 native strains screened against neonate of BSFB (data not shown here). Thus, BtVKK-BB2 strain was selected for gfp tagging through electroporation. This strain is resistant to ampicillin and penicillin therefore maintained on Luria Bertani agar (LA) supplemented with ampicillin and penicillin (HiMedia Laboratories Pvt Ltd, India) at 50 µg/ml at 30 °C. The Escherichia coli harboring pCAMBIA1302 plasmid (Fig. 1) was grown in Luria Bertani broth (LB) supplemented with 50 µg/ml kanamycin (HiMedia Laboratories Pvt Ltd, India) at 37 °C.
3
Enterococcus Antibiotic Susceptibility Patterns
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The present study was conducted at Vinayaka Mission's Medical College and Hospitals, Salem Institutional Ethical Committee (IEC) Clearance (MICROBIOLOGY/Ph.D/01/2015) was obtained prior to this study A total of 1200 clinical samples received at the Department of Microbiology for routine culture and sensitivity were included. A total of 774 isolates of Enterococci obtained from 1200 clinical samples were processed for species identification and antimicrobial sensitivity testing. Identification of isolates to the species level was performed by the sugar utilization test using brain heart infusion broth incorporated with 1% sugar and bromothymol blue indicator.[9 (link)] Isolates of Enterococci were subjected to antimicrobial susceptibility testing by the Kirby–Bauer disc diffusion method as per Clinical and Laboratory Standards Institute guidelines[10 ] using the following drugs: penicillin (10 U), erythromycin (15 μg), high-level gentamicin (120 μg), linezolid (30 μg), teicoplanin (30 μg), ciprofloxacin (5 μg), and nitrofurantoin (300 μg).(Himedia Laboratories Ltd.) The minimum inhibitory concentrations (MICs) for these antibiotics were also determined bythe Vitek automated system.
4
Chrysin and Sodium Selenite Cytotoxicity Study
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Chrysin (97% pure) was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO), sodium selenite (Na2SeO3) was obtained from LOBA Chemie (Mumbai, India), and Dulbecco’s modified Eagle’s medium (DMEM) and the antibiotics streptomycin and penicillin were obtained from HiMedia (Mumbai, India). Antibodies for m-calpain, Lp82, EGR-1, COX-1, caspase-3, caspase-8, caspase-9, and β-actin were purchased from Sigma-Aldrich Chemical Co. and Cell Signaling Technologies (CST; Danvers, MA). Anti-rabbit immunoglobulin (IgG) secondary antibody was obtained from Genei (Bengaluru, India). All other chemicals and reagents were of analytical grade. Milli-Q water (Millipore, Bengaluru, India) was used throughout the experiments.
5
Comprehensive Cell Culture Reagent Procurement
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Cell culture media components such as DMEM, RPMI-1640 medium, Ham’s F12 K medium, penicillin, streptomycin, Hi-Sep Lymphocyte separation medium, HiKaryo XL RPMI medium, MTT, acridine orange, BSA, skimmed milk, prestained protein marker and tween 20 were purchased from HiMedia Laboratories, Mumbai, India. DMSO, trypan blue, Hoechst 33258, DCFH-DA, Rhodamine-123, Tris base, TRI reagent, Oligo(dT)-18 mer primers, Protease/phosphatase Inhibitor Cocktail and KBr were purchased from Sigma-Aldrich, USA. Fetal bovine serum, Dulbecco’s Phosphate Buffered Saline (DPBS), Trypsin–EDTA, Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit, Fluo-3 AM, propidium iodide and Geltrex LDEV-Free Reduced Growth Factor Basement Membrane Matrix were purchased from Thermo Fisher Scientific, USA. M-MuLV reverse transcriptase enzyme, RNase A, dNTP mix and agarose were purchased from Genei Laboratories Pvt. Ltd, Bangalore, India. Triton X-100, EDTA, glycine, ethidium bromide, BCIP, NBT, silica gel for column chromatography (60–120 meshes), vanillin, sulphuric acid and solvents such as petroleum ether, chloroform, acetone, methanol (all HPLC grade) were procured from Sisco Research Laboratories Pvt. Ltd., Mumbai, India. Antibodies were procured from Cell Signaling Technology, Inc., USA and Sigma-Aldrich, USA. Primers sets were purchased from Eurofins Genomics India, Bangalore, India.
6
Rat Pheochromocytoma Cell Culture
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Roswell Park Memorial Institute (culture medium) (RPMI1640), Fetal bovine serum, penicillin (100 U/ml), and streptomycin (100 μg/ml) were purchased from Himedia, India. The general chemicals were also purchased from Sigma or Merck, India. The rat pheochromocytoma (PC12) cells line used in the present study were obtained from the National Center for Cell Science, Pune, India.
7
Murine T Cell Activation Assay
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FK506 (Cat. no. F4679-5MG), Concanavalin A (ConA) (Cat. no. C0412-5MG), 5 -IRTX (Cat. no. I9281-1MG) were purchased from Sigma Aldrich (St. Louis, MO, USA); antimouse CD25 (Cat. no. 553866), OptEIA kits for IL-2 (Cat. no.555148), , TNF (Cat. no. 558534) for sandwich ELISA were from BD Biosciences, SJ, CA, USA.; antimouse CD69 (Cat. no. 35-0691-U100), anti-mouse CD90.2 (Cat. no. 20-0903-U100) from Tonbo Biosciences, San Diego, CA, USA; anti-mouse TRPV1 (Cat. no. ACC-029) from Alomone Laboratories (Jerusalem, Israel); anti-mouse CD3 (Cat. no. 40-0032-U500) and antimouse CD28 (Cat. no. 40-0281-U500) [functional assay grade] were procured from Tonbo Biosciences, San Diego, CA, USA. Secondary anti-rabbit Alexa Fluor 488 and T cell isolation kit (Dynabeads™ Untouched™ Mouse T Cells Kit, Cat. no. 11413D) were procured from Invitrogen, Carlsbad, CA USA. RPMI-1640 cell culture medium and FBS were purchased from PAN Biotech, Aiden Bach, Germany; DMEM cell culture medium, 10X RBC lysis buffer, 10X PBS, L-glutamine, penicillin, streptomycin was from Himedia Laboratories, Mumbai, Maharashtra, India.
8
Cytotoxicity and Cell Cycle Analysis of PLL and MTT
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PLL hydrobromide (Mrs 30,000 – 70,000) and 3− (4, 5-Dimethytthiazol-2-yl) −2, 5-dipheneyltetrazolim bromide (MTT) was obtained from Sigma-Aldrich (USA). Giemsa stain, Haematoxylin and Eosin stain, Papanicalaou stain were obtained from Qualigene (Mumbai, India). Cell cycle reagent was purchased from Merck Millipore (USA). Terminal deoxynucleoitidyltransferase-mediated dUTP nick end labeling kits were from BD Bioscience (USA), Propidium Iodide (PI), acridine orange, 4’-6 – diamidino-2-pheny lindole (DAPI) were purchased from Sigma Chemical Co. (St. Louis, USA). Genomic DNA purification kit was purchased from Fermentas (USA). Annexin V and primary antibodies were obtained from BD Bioscience (USA). Secondary anti-bodies were purchased from Sigma (USA). Dulbeceo’s modified Eagle medium (DMEM), Penicillin, streptomycin and neomycin (PSN), fetal bovine serum (FBS), trypsin and ethylene di-amine tetra-acetic acid (EDTA), Trypan blue were obtained from HiMedia (India). A primary antibody for immunehistochemistry study CD31 was obtained from Santa Cruz Biotechnol. Inc., CA USA. Bcl-2 antibody was purchased from Anaspec, USA. p53 was purchased from Pathn Situ Biotechnologies, India.
9
Cytotoxicity Assay of Paclitaxel on A549 Cells
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Dulbecco’s Modified Eagle Medium (DMEM), Penicillin and Streptomycin purchased from Himedia, Mumbai; Fetal Calf Serum (FCS) from Lonza, Belgium; DMSO (dimethyl sulfoxide) from Sigma-Aldrich, USA; MTT reagent [3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] from Merck, India and Paclitaxel from Dabur, India. Cell lines were procured from National Centre for Cell Science (NCCS), Pune, India. A549 human lung cancer cells were grown in DMEM supplemented with 100 U/mL, Penicillin G, 100 lg/mL Streptomycin, 0.25 lg/mL, Amphotericin, and 10% heat inactivated fetal bovine serum. Cultures were maintained at 37 °C in a 5% CO2, 95% air atmosphere.
10
Antibiotic Susceptibility Screening of Isolates
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Antibiotic drug susceptibility was determined by spreading overnight grown culture of the isolates on MRS agar plates as a lawn. Standard antibiotic discs (tetracycline, erythromycin, ampicillin, gentamycin, penicillin, chloramphenicol, cefuroxime, cefoperazone, levofloxacin, norfloxacin, Hi-Media, Mumbai) were placed on the surface of the MRS agar medium aseptically. Plates were incubated for 24 h at 37 °C and observed for zones of inhibition.
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