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46 protocols using ethyl alcohol

1

Bacterial Cellulose Hydrolysis and Analysis

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Bacterial cellulose was provided by Hainan Yida Co., Ltd. (Guangdong, China). Cellulase from Aspergillus niger (product #C0057, 10 000 U/g, slightly brown powder) was purchased from Okyo Chemical Industry (TCI). The following chemicals were purchased from Sinopharm Chemical Reagent Co., Ltd: dimethylacetamide (DMAc, ≥ 99%), lithium chloride (LiCl, ≥97%), sulfuric acid (≥98%), Avicel citric acid (≥99.5%), sodium citrate dihydrate (meets USP testing specifications), 3,5-dinitrosalicylic acid (DNS, ≥98%), ethyl alcohol (EtOH, ≥99%), acetone (≥
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Synthesis of Silica-based Biosensor Materials

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Ethanediamine (EDA, 99%), formaldehyde (30–40%), ethyl alcohol (99.7%), 3-aminophenol (99%), ethylenediamine tetraacetic acid disodium salt (EDTA·2Na, 99%), H2SO4 (95–98%), HCl (36–38%), NH3·H2O (25–28%), and BaCl2·2H2O (99.5%) were purchased from Sinopharm Chemical Reagent (Shanghai, China). NaOH (96%) and Na2SO4 (99%) were purchased from Shanghai Dahe Chemicals (Shanghai, China). NaH2PO4·2H2O (99%), and Na2HPO4·12H2O (99%) were purchased from Chinasun Specialty Product (Changshu, China). Tetraethoxysilane (TEOS, 99%), catalase (from bovine liver, 2000–5000 units/mg protein), 3,3′,5,5′-tetramethylbenzidine (TMB, 98%), and peroxidase from horseradish (HRP, ~200 units/mg) were purchased from Sigma–Aldrich (St. Louis, MO, USA). All chemicals were used without further purification.
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Synthesis of Acetylacetone Cobalt(II) Catalyst

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All chemical regents including concentrated hydrochloric acid (HCl), polyvinyl alcohol, ethyl alcohol and acetylacetone cobalt(II)(AACo) were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Olive oil (Olivoilà) and deionized water obtained from a Milli-Q system (Millipore, Bedford, MA) were used.
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4

Analytical Reagents for Furfural Conversion

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Analytical reagents, including furfural, dichloromethane, tetrahydrofuran, methyl alcohol, ethyl alcohol, sodium hydroxide, sodium carbonate, aluminium nitrate nonahydrate, magnesium nitrate hexahydrate, cobaltous nitrate hexahydrate, ziconyl nitrate, calcium oxide, magnesium oxide, gamma aluminum oxide and potassium fluoride dihydrate were purchased from Sinopharm Chemical Reagent Co., Ltd. Cyclopentanone (99%) was purchased from Shanghai Aladdin Bio-Chem Technology Co., Ltd. F2C standard was prepared according to the report,30 (link) and verified by nuclear magnetic resonance (NMR). furfural was directly used without any preliminary treatment considering the same experimental result.25 (link)
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5

Synthesis and Characterization of Bismuth Complexes

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Bi(NO3)3·5H2O (AR, 99.0%), glycol (AR, 99.5%), and methanol (AR, 99.5%) were purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Na2MoO4·2H2O (AR, 99.0%), TEMDA (AR, 99.5%), ethyl alcohol (AR, 99.5%) and tetrahydrofuran (AR, 99.5%) were purchased from Sinopharm Chemical Reagent Co., Ltd., SCRC, China. BDE209 (Fig. S1) was obtained from Aldrich Chemical Company (USA). All chemicals were used without further purification.
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6

Yeast Fermentation Optimization Protocol

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Budding yeast (Saccharomyces cerevisiae) was purchased from the Angel Yeast Co., Ltd. (Yichang, China). Fructose (99%) and sucrose (99%) were purchased from the Sigma-Aldrich Co., Ltd. (Missouri, USA). Inulin (90%) was purchased from the Yuan Ye Biotechnology Co., Ltd. (Shanghai, China) and 5-HMF (99%) was purchased from the Aladdin Chemicals Co., Ltd. (California, USA). Acetone (99%), ethyl alcohol (99%), ethyl acetate (99%), and concentrated sulphuric acid (98%) were obtained from the Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Methanol (HPLC grade) was purchased from the Fisher Chemicals Co., Ltd. (New Jersey, USA). [BMIM][Cl] (99%) was purchased from the Linzhou Keneng Materials Technology Co., Ltd. (Linzhou, China). Ultrapure water was used in all experiments. All chemicals were used as obtained without further treatment.
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Synthesis of Polymer Composite Materials

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Phenylethylene, sodium p-styrene sulfonate, sodium bicarbonate, sodium hydroxide, potassium persulfate, citric acid monohydrate, ethyl alcohol, ethylene glycol, hydrochloric acid, methanol and cobalt nitrate hexahydrate were all purchased from Sinopharm Chemical Reagent Co. Ltd. All the chemicals and materials was analytically pure and applied without further treatment. Deionized water was used in all experiments.
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8

Synthesis of Metal Nitrate Complexes

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Cu(NO 3 ) 2 •6H 2 O, Co(NO 3 ) 2 •6H 2 O and Zn(NO 3 ) 2 •6H 2 O were purchased from Adamas Reagent Co., Ltd. Dimethylimidazole was obtained from TCI (Shanghai) Development Co., Ltd. Furfural, furfuryl alcohol, tetrahydrofuran, 2-propanol, methyl alcohol, ethyl alcohol, acetonitrile, methylbenzene and 1,4-dioxane were purchased from Sinopharm Chemical Reagent Co., Ltd. In addition, the commercial noble metals catalysts were purchased from Aladdin Reagent. All chemicals were used without further treatments.
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9

Sensitive Aflatoxin B1 Detection

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AFB1 standard solution, (8 mg•mL -1 solution in methanol and working dilution by deionized water),AFB1-BSA antigen (extent of labeling 8-12 mol Aflatoxin B1 per mol BSA), monoclonal anti-AFB1 antibody, (6 mg•mL -1 solution and working dilution by phosphate buffer solution) was obtained from Beijing Mozhidong Bio-tech (city. Country). Hydrated rare earth nitrate (RECl3•xH2O, RE Y, Yb, Er, ≥ 99.99%), oleic acid (≥ 90%) and octadecanoic acid (≥ 90%) were purchased from Sigma-Aldrich (Shanghai, China). In addition, FeCl3•6H2O, sodium fluoride, sodium hydroxide, methyl alcohol, toluene, ethyl alcohol, sodium citrate, 1,6-hexanediamine, anhydrous sodium acetate, glycol, bovine serum albumin (BSA, 96-99%),25% glutaraldehyde, tetraethyl orthosilicate (TEOS ≥ 98%), and 3-aminopropyltrimethoxysilane (APTES) was all purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All the chemicals used were of analytical grade. The water used was deionized.
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10

Radish and Carp Gelatin Hydrogel Protocol

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Fresh red radish (cultivar 'Xinlimei') and live black carp were purchased from local market, and pasteurized milk was purchased from a local cattle farm (Zhenjiang, China). Gelatin (type B, pig skin) was purchased from Sigma-Aldrich Inc.
(St. Louis, MO, USA). Low-acyl gellan gum was purchased from Dancheng Caixin sugar industry Co., ltd. (Dancheng, China). Other chemical agents, such as ethyl alcohol, calcium chloride, acetic acid, ammonium hydroxide, acetonitrile and formic acid were bought from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
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