We used the data from the average of percentage freezing during the initial 6 min on day 3 and on day 28 as TEST and RE, respectively. Further, we used the data from the average of percentage freezing at 2-min intervals in each extinction session to measure and calculate fear extinction. For fear conditioning and extinction, we used one-way (group) and multiple (bin, day and group) analysis of variance (ANOVA) to detect significant differences, respectively.
In the OFT we used the data from the average of moving distance at 2-min intervals and the total duration time in the center area to measure the total distance and center time, respectively. For the center time and total distance, we used a one-way (group) ANOVA or Student's t-test and a two-way (time and group) repeated ANOVA, respectively, for significant differences. We used a post-hoc Bonferroni test or Dunnett's test for multiple comparisons. For all analyses, the level of statistical significance was set at p<0.05. All statistical were performed using
SPSS 22.0 J for Windows (SPSS Japan, Tokyo). The data are presented at mean ± SEM.
Mizuno I., Matsuda S., Tohyama S, & Mizutani A. (2022). The role of the cannabinoid system in fear memory and extinction in male and female mice. Psychoneuroendocrinology, 138.