Rat igg2a k isotype control apc

Manufactured by Thermo Fisher Scientific

Sourced in United States

Rat IgG2a K Isotype Control APC is a lab equipment product used as a control in flow cytometry experiments to help determine the specificity of antibody staining. It is an allophycocyanin (APC) conjugated isotype control antibody derived from rat IgG2a immunoglobulin.

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Lab products found in correlation

Lsrfortessa, by BD (1 mentions) Anti mouse rat foxp3 apc, by Thermo Fisher Scientific (1 mentions) Histopaque 1083, by Merck Group (1 mentions) Biotinylated griffonia simplicifolia lectin 1 isolectin b4, by Vector Laboratories (1 mentions) Egm 2 medium, by Lonza (1 mentions)

Spelling variants of this product

IgG2a (72 citations) Isotype controls (54 citations) Rat IgG2a (30 citations) IgG2a isotype control (2 citations) IgG2a APC (2 citations)

2 protocols using rat igg2a k isotype control apc

Immune Cell Profiling by Flow Cytometry

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Peripheral blood mononuclear cell suspensions were analyzed using BD LSRFortessa custom flow cytometer (LSRFortessa SORP, Becton, Dickinson and Company), using the following antibodies: Rat CD4 APC-Cy7 OX-35, MS IGG2A KPA ITCL APC-CY7 G155-178, Rat IL-4 PE OX-81, Ms IgG1 Kpa ItCl PE MOPC-21, Rat IFN-Gma FITC DB-1, Ms IgG1 Kpa ItCl FITC MOPC-21, Rat CD25 BV421 OX-39, Mouse IgG1 Kpa ItCl BV421 X40 (BD Pharmingen, USA), Anti-Mouse/Rat IL-17A PerCP-Cyanine5.5, Rat IgG2a K Isotype Control PerCP-Cyanine5.5, Anti-Mouse/Rat Foxp3 APC, Rat IgG2a K Isotype Control APC (eBioscience, USA), and Transcription Factor Buffer Set, Leuko Act Cktl With GolgiPlug (BD Pharmingen, USA).The expression levels of Th1, Th2, Th17, and Treg cells were detected by the expression levels of CD3⁺CD4⁺IFN-γ⁺, CD3⁺CD4⁺IL-4⁺, CD3⁺CD4⁺IL-17A⁺, CD4⁺CD25⁺Foxp3⁺, respectively.

Peng J., Lu X., Xie K., Xu Y., He R., Guo L., Han Y., Wu S., Dong X., Lu Y., Liu Z., Cao W, & Gong M. (2019). Dynamic Alterations in the Gut Microbiota of Collagen-Induced Arthritis Rats Following the Prolonged Administration of Total Glucosides of Paeony. Frontiers in Cellular and Infection Microbiology, 9, 204.

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Isolation and Analysis of Murine Stem Cells

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EGM-2 Medium was purchased from Lonza corporation (CC-3202). Histopaque^®-1083 was from Sigma-Aldrich (10831). FAM-YVAD-FMK caspase-1 detection kit was from Cell Technology (FAM600-2). Anti-APC MultiSort kit and MicroBeads were from MACS (130-091-255/130-048-801). Rat IgG2a K Isotype control APC was from eBioscience (17-4321-81). Biotinylated Griffonia simplicifolia lectin I (isolectin B4) from Vector (B-1205), Biotin-XX conjugate was from Invitrogen (I21414). APC anti-mouse Ly-6A/E (Sca-1) antibody were from Biolegend (121906/108112). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO).

Li Y.F., Huang X., Li X., Gong R., Yin Y., Nelson J., Gao E., Zhang H., Hoffman N.E., Houser S.R., Madesh M., Tilley D.G., Choi E.T., Jiang X., Huang C.X., Wang H, & Yang X.F. (2016). Caspase-1 mediates hyperlipidemia-weakened progenitor cell vessel repair. Frontiers in bioscience (Landmark edition), 21, 178-191.

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