S9.6 mabe1095

Manufactured by Merck Group

Sourced in United States

The S9.6 (MABE1095) is a lab equipment product manufactured by Merck Group. It is designed to perform a core function, but a detailed description while maintaining an unbiased and factual approach is not available.

Automatically generated - may contain errors

Lab products found in correlation

Ab171870, by Abcam (1 mentions) Ab18413, by Abcam (1 mentions) Ab5131, by Abcam (1 mentions) Gapdh sc 47724, by Santa Cruz Biotechnology (1 mentions) γh2ax, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

MABE1095 (22 citations)

2 protocols using s9.6 mabe1095

Investigating lncRNA BM450697 Regulation

Cited 1 time

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Pol II phosphorylated serine 5 C-terminal repeat domain (CTD; ab5131) and its respective rabbit IgG control antibody (ab171870), as well as mouse IgG controls (ab18413), were purchased from Abcam (Cambridge, UK). SREBP1 chip grade antibody (sc-13551X) was purchased from Santa Cruz (Dallas, TX, USA). S9.6 (MABE1095) was purchased from Merck Millipore (Burlington, MA, USA). A BM450697 expression vector was synthesized by GeneWiz (South Plainfield, NJ, USA) in a pcDNA3.1+ backbone. Ten all-natural siRNAs, designed to target the lncRNA BM450697 or its putative promoter region,³⁰ (link) were synthesized by Integrated Technologies (Skokie, IL, USA). Based on initial screening analysis, two siRNA pairs (5 and p5) were chosen for further investigation.

Ray R.M., Hansen A.H., Slott S., Taskova M., Astakhova K, & Morris K.V. (2019). Control of LDL Uptake in Human Cells by Targeting the LDLR Regulatory Long Non-coding RNA BM450697. Molecular Therapy. Nucleic Acids, 17, 264-276.

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DNA Damage Response Protein Analysis

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The antibodies were used where indicated. pChk1 S317(#12302), pChk1 S345 (#2348), pChk2(#2661), Chk2 (#3440), [pS/pT] QG (#6966) and γH2AX (#9718) were obtained from Cell Signaling Technology; ATR (A300-138A), ATM (A300-299A), Rad17 pS645 (A300-153A), RPA32 (A300-244A), pRPA32 S33 (A300-246A) and pRPA32 S4/8 (A300-245A) antibodies were from Bethyl Lab; BRCA1 (sc-6954), DHX9 (sc-137232), FLAG (sc-166355), Chk1 (sc-8408), PARP1 (sc-8007), PRP19 (sc-514338), PCNA (sc-56) and GAPDH (sc-47724) antibodies from Santa Cruz; DHX9 (ab26271) were purchased from abcam; FLAG (F7425), γH2AX (05–636), α-Tubulin (T6074) and S9.6 (MABE1095) were purchased from Merck; RPA32 (MA1-26418), goat anti-mouse-HRP (G21040), goat anti-rabbit-HRP (G21234), goat anti-mouse Alexa Fluor-594 (A-11005) and goat anti-rabbit Alexa Fluor-488 (A-11034) were from ThermoFisher; pATR (GTX128145), pATM (GTX132146), RPA70 (GTX108749) were obtained from GeneTex; HRP conjugated anti-mouse (115-035-174) and anti-rabbit (211-032-171), light chain specific antibodies were obtained from Jackson ImmunoResearch; affinity-purified DHX9 pS321 and pS688 were customized by GeneTex.

Liu M.Y., Lin K.R., Chien Y.L., Yang B.Z., Tsui L.Y., Chu H.P, & Wu C.S. (2023). ATR phosphorylates DHX9 at serine 321 to suppress R-loop accumulation upon genotoxic stress. Nucleic Acids Research, 52(1), 204-222.

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