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A0166

Manufactured by Merck Group
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Sourced in United States, Israel

A0166 is a laboratory equipment product manufactured by Merck Group. It is a versatile instrument designed for various scientific applications. The core function of A0166 is to perform precise measurements and analysis, but details regarding its intended use are not provided in this response.

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Lab products found in correlation

Ampicillin, by Merck Group (2 mentions) M3761, by Merck Group (1 mentions) Kanamycin, by Merck Group (1 mentions) E5389, by Merck Group (1 mentions) Erythromycin, by Merck Group (1 mentions) Tetracyclin, by Merck Group (1 mentions) Lb agar, by Carl Roth (1 mentions) T7660, by Merck Group (1 mentions) Mrs agar, by Carl Roth (1 mentions) Metronidazole, by Thermo Fisher Scientific (1 mentions) Neomycin trisulfate, by Merck Group (1 mentions)

5 protocols using a0166

1

Antibiotic-Mediated Microbiome Depletion

Cited 3 times
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To allow an efficient colonization of the donor microbiota by initially depleting native microbiota, all recipients were administered an antibiotic cocktail of ampicillin (1 g/L; #A0166, Sigma-Aldrich, USA), vancomycin (500 mg/L; #PHR1732, Sigma-Aldrich, USA) neomycin (1 g/L; #N6386, Sigma-Aldrich, USA), and metronidazole (1 g/L; #M3761, Sigma-Aldrich, USA) (AVNM), as described previously (48 (link)), for a total of 9 days in drinking water. This antibiotic cocktail regimen has been shown to be effective in reducing up to 90% of the native bacterial community and depletes most groups of microbes (e.g., gram positive, gram negative and anaerobes) in male and female mice (49 (link)–51 (link)).
Acharya K.D., Friedline R.H., Ward D.V., Graham M.E., Tauer L., Zheng D., Hu X., de Vos W.M., McCormick B.A., Kim J.K, & Tetel M.J. (2022). Differential effects of Akkermansia-enriched fecal microbiota transplant on energy balance in female mice on high-fat diet. Frontiers in Endocrinology, 13, 1010806.
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2

Antibiotic-Induced Gut Microbiome Modulation

Cited 1 time
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Seven-to-eight-week-old female mice (B6J) were treated for 10 consecutive days with ampicillin (1 g l−1; A0166 Sigma), vancomycin (500 mg l−1; A1839,0001 Applichem), neomycin sulfate (1 g l−1; 4801 Applichem), and metronidazole (1 g l−1; H60258 Alfa Aesar) in autoclaved drinking water, as previously described41 (link). Water bottles were monitored and refilled twice per week.
Gurtner A., Borrelli C., Gonzalez-Perez I., Bach K., Acar I.E., Núñez N.G., Crepaz D., Handler K., Vu V.P., Lafzi A., Stirm K., Raju D., Gschwend J., Basler K., Schneider C., Slack E., Valenta T., Becher B., Krebs P., Moor A.E, & Arnold I.C. (2022). Active eosinophils regulate host defence and immune responses in colitis. Nature, 615(7950), 151-157.
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3

Phage-based Detection of Y. pestis

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Y. pestis strains used in this study are listed in Table 1. The Kim53 and Kim53Δ70Δ10 strains were grown on Brain Heart Infusion Agar (BHIA; BD, MD, USA), and Kim53-lux and EV76-lux were routinely grown on BHIA supplemented with ampicillin (200 µg/mL, A0166, Sigma–Aldrich, Rehovot, Israel).
The Y. pestis-specific lytic phages used in this study were фA1122 (accession no. NC004777, kindly provided by professor Mikael Skurnik [26 (link)]), and PST (ATCC, cat. no.: 23207-B1; accession no. KF208315).
Vagima Y., Gur D., Aftalion M., Moses S., Levy Y., Makovitzki A., Holtzman T., Oren Z., Segula Y., Fatelevich E., Tidhar A., Zauberman A., Rotem S., Mamroud E, & Steinberger-Levy I. (2022). Phage Therapy Potentiates Second-Line Antibiotic Treatment against Pneumonic Plague. Viruses, 14(4), 688.
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4

Germ-Free Fly Maintenance Protocol

Cited 1 time
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Flies were rendered germ-free (GF) following the protocol described in49 (link). GF flies were maintained on fly medium supplemented with antibiotics: kanamycin 50μg/mL (Sigma #K1377), ampicillin50μg/mL (Sigma #A0166), tetracyclin 10μg/mL (Sigma #T7660), erythromycin 5μg/mL (Sigma #E5389). Axenicity was confirmed by crushing the flies and plating the lysate on LB Agar (Carl Roth) and MRS Agar (Carl Roth) plates.
Redhai S., Pilgrim C., Gaspar P., van Giesen L., Lopes T., Riabinina O., Grenier T., Milona A., Chanana B., Swadling J.B., Wang Y.F., Dahalan F., Yuan M., Wilsch-Brauninger M., Lin W.H., Dennison N., Capriotti P., Lawniczak M.K., Baines R.A., Warnecke T., Windbichler N., Leulier F., Bellono N, & Miguel-Aliaga I. (2020). An intestinal zinc sensor regulates food intake and developmental growth. Nature, 580(7802), 263-268.
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5

High-Fat Diet Induced Obesity Mouse Model

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11 week-old C57Bl/6J mice were reared on a high-fat diet (RD12492). After 1 week of acclimation, these DIO mice were weight matched into two groups – Sham or SG. Two days prior to surgery, mice were administered an antibiotic cocktail in drinking water – neomycin trisulfate (1g/L, Sigma Aldrich N5285, St. Louis, MO), metronidazole (0.25 g/L, Acros Organics, 210340050, NJ), vancomycin hydrochloride (0.5g/L, Acros Organics 210340050, NJ), and ampicillin (1 g/L, Sigma Aldrich A0166, St. Louis, MO). Water containing antibiotics was mixed with sterile water, brought to a pH of 7.4, and provided to mice ad libitum in light-protected bottles. This treatment was continued in the post-operative setting. The antibiotic-containing water was changed three times weekly. Stool pellets were collected prior to antibiotic initiation and at regular intervals post initiation and throughout the duration of the experiment.
Chaudhari S.N., Luo J.N., Harris D.A., Aliakbarian H., Yao L., Paik D., Subramaniam R., Adhikari A.A., Vernon A.H., Kiliç A., Weiss S.T., Huh J.R., Sheu E.G, & Devlin A.S. (2021). A microbial metabolite remodels the gut-liver axis following bariatric surgery. Cell host & microbe, 29(3), 408-424.e7.
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