Viruses were filtered through 0.45 µm PES membrane filters (Nalgene, Conway, NH, USA) and concentrated down to 50 mL for purification. Viruses were purified over 20% sucrose cushion and ultracentrifuged at 25,000 rpm in a SW32 rotor for 1.5 h. Viral particles were resuspended in TEN buffer + 2% sucrose and inactivated with 50 µL per mg of particles with β-propiolactone (BPL, Millipore Sigma, Cat# P5648, Burlington, VT, USA) in cold culture grade water. The level of inactivation was verified by inoculating Vero CCL81 cells over three passages with 10 µg of BPL-inactivated virions.
Beta propiolactone bpl
Beta-propiolactone (BPL) is a colorless, oily liquid used as a chemical intermediate in various industrial and laboratory applications. It serves as a cross-linking agent and disinfectant. BPL is commonly used in the synthesis of other organic compounds and for sterilization purposes in laboratory settings.
Lab products found in correlation
13 protocols using beta propiolactone bpl
Rabies Virus Production and Purification
Viruses were filtered through 0.45 µm PES membrane filters (Nalgene, Conway, NH, USA) and concentrated down to 50 mL for purification. Viruses were purified over 20% sucrose cushion and ultracentrifuged at 25,000 rpm in a SW32 rotor for 1.5 h. Viral particles were resuspended in TEN buffer + 2% sucrose and inactivated with 50 µL per mg of particles with β-propiolactone (BPL, Millipore Sigma, Cat# P5648, Burlington, VT, USA) in cold culture grade water. The level of inactivation was verified by inoculating Vero CCL81 cells over three passages with 10 µg of BPL-inactivated virions.
Inactivated viral vaccine production
Rabies Virus Immunization and Antibody Response
Influenza Virus Propagation and Inactivation
Inactivated H9N2 virus vaccine protocol
Chickens and shelducks aged 10–12 weeks old were hatched from SPF embryos (chicken embryos were obtained from Beijing Merial Vital Laboratory Animal Technology Co., Beijing; duck embryos were obtained from Harbin Veterinary Research, Harbin) and housed in isolators until further analysis.
Inactivation of LASSARAB and FILORAB1 Vaccines
Zika Virus Strains Isolation and Characterization
BRBV Neutralizing Antibody Generation
Serum Antibody Detection for H7N9 Influenza
BPL Inactivation of PRRSV-1 Viral Stock
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