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20 hete

Manufactured by Merck Group
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20-HETE is a laboratory reagent used for research purposes. It is a metabolite of arachidonic acid produced by cytochrome P450 enzymes. 20-HETE plays a role in the regulation of various physiological processes, but its specific functions are subject to ongoing research and investigation.

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Lab products found in correlation

Gc 17a, by Shimadzu (2 mentions) Osmomat 030, by Gonotec (2 mentions) Trizol kit, by Thermo Fisher Scientific (1 mentions) Goat anti mouse igg, by Santa Cruz Biotechnology (1 mentions) Clofibrate, by Merck Group (1 mentions) Het0016, by Merck Group (1 mentions) Streptavidin peroxidase kit, by Zhongshan Biotechnology (1 mentions)

3 protocols using 20 hete

1

Measurement of Urinary 20-HETE

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Urine osmolality was determined by freezing-point depression using a semi-micro osmometer (Osmomat 030, Gonotec, Germany) and sodium concentrations by flame photometer (Jenway PFP7, Essex, UK). 20-HETE concentration in urine samples was measured by gas chromatography (Shimadzu GC-17A, Shimadzu, Japan) using own calibration standards prepared from synthetic 20-HETE (Sigma, USA).
Walkowska A., Kuczeriszka M., Sadowski J., Olszyński K.H., Dobrowolski L., Červenka L., Hammock B.D, & Kompanowska-Jezierska E. (2015). High Salt Intake Increases Blood Pressure in Normal Rats: Putative Role of 20-HETE and No Evidence on Changes in Renal Vascular Reactivity. Kidney & blood pressure research, 40(3), 323-334.
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2

Measurement of Urinary 20-HETE

Check if the same lab product or an alternative is used in the 5 most similar protocols
Urine osmolality was determined by freezing-point depression using a semi-micro osmometer (Osmomat 030, Gonotec, Germany) and sodium concentrations by flame photometer (Jenway PFP7, Essex, UK). 20-HETE concentration in urine samples was measured by gas chromatography (Shimadzu GC-17A, Shimadzu, Japan) using own calibration standards prepared from synthetic 20-HETE (Sigma, USA).
Walkowska A., Kuczeriszka M., Sadowski J., Olszyński K.H., Dobrowolski L., Červenka L., Hammock B.D, & Kompanowska-Jezierska E. (2015). High Salt Intake Increases Blood Pressure in Normal Rats: Putative Role of 20-HETE and No Evidence on Changes in Renal Vascular Reactivity. Kidney & blood pressure research, 40(3), 323-334.
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3

Measuring CYP4A11 Enzyme Activity

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CP, 20-HETE, HET-0016, and clofibrate were obtained from Sigma (St. Louis, MO, USA). Dulbecco's modified eagle medium (DMEM), ECL chemiluminescence kit and SYBR Green Real-time PCR Master Mix were purchased from Toyobo Company (Tokyo, Japan). Trizol kit was purchased from Invitrogen (Carlsbad, CA, USA). CYP4A11 monoclonal antibody and goat anti-mouse IgG were obtained from Santa Cruz (Carlsbad, CA, USA). Streptavidin peroxidase kit, LDH measurement kit, and protease inhibitor cocktail were purchased from Beijing Zhongshan Biotechnology Co. Ltd. (Beijing, China).
Li J., Li D., Tie C., Wu J., Wu Q, & Li Q. (2015). Cisplatin-mediated cytotoxicity through inducing CYP4A 11 expression in human renal tubular epithelial cells. The Journal of toxicological sciences, 40(6).
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