One touch

Oral glucose tolerance test (OGTT) For OGTT, at mornings of days 90 (adult life) and 120 (adult life and early period of mating), after fasting for six hours, a glucose solution (200 g/L) was administered by gavage at a final dose of 2 g/kg body weight. Following, the blood samples were obtained by a small cut tip of the tail for blood glucose levels determination using a specific glucometer (OneTouch Ultra-Johnson & Johnson ® ) in the first timepoint (zero). For obtaining of the blood samples at 30, 60, and 120 minutes (min), a massage on the tail was performed toward the same small cut tip. The glycemic values were expressed in mg/dL 7 (Figure 1). Physiological and biochemical parameters analyzed before pregnancy (pre pregnancy period) At mornings of days 91 and 121 of life, maternal body weights, post prandial glycemia, triglyceride and total cholesterol levels were determined. All blood samples were obtained from a small cut tip tail. Blood glucose concentrations were measured by conventional glucometer and these values were expressed in mg/dL. For evaluation of triglycerides and total cholesterol levels, Accutrend ® Plus (Roche Diagnostics GmbH, Mannheim, Germany) was used and the values were also expressed as mg/dL. The 24h-chow (g) and water (mL) intakes were calculated.