RNA was isolated using Trizol reagent (Molecular research center, Inc.) according to manufacturer’s instructions. RNA integrity was verified using RNA chip (Agilent bioanalyzer). 1 µg of RNA was used to synthesize cDNA (20 µl reaction) (Biorad’s Iscript select cDNA synthesis kit) according to manufacturer’s protocol. The cDNA was diluted 1:10 and used for QPCR with SYBR green detection (Invitrogen). Phusion HF reaction buffer, phusion HF DNA polymerase and dNTPs used for QPCR were purchased from New England Biolabs. Biorad CFX96 Touch real-time PCR detection system was used. Biorad CFX manger software was used to calculate fold changes in gene expression. All primers sequences were listed in supplementary methods . All NMG are defined in supplementary table 3 .
Biorad cfx manger software
Manufactured by Bio-Rad
The Bio-Rad CFX Manager software is a data analysis software designed to work with Bio-Rad's CFX real-time PCR detection systems. It provides users with tools to set up, run, and analyze real-time PCR experiments.
Automatically generated - may contain errors
Lab products found in correlation
Phusion hf dna polymerase, by New England Biolabs (2 mentions)
Iscript select cdna synthesis kit, by Bio-Rad (2 mentions)
Sybr green detection, by Thermo Fisher Scientific (2 mentions)
Phusion hf reaction buffer, by New England Biolabs (2 mentions)
Cfx96 touch real time pcr detection system, by Bio-Rad (2 mentions)
Dntps, by New England Biolabs (2 mentions)
Trizol reagent, by Molecular Research Center (2 mentions)
2 protocols using biorad cfx manger software
1
RNA Isolation, cDNA Synthesis, and qPCR Analysis
2
RNA Isolation, cDNA Synthesis, and qPCR Analysis
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RNA was isolated using Trizol reagent (Molecular research center, Inc.) according to manufacturer’s instructions. RNA integrity was verified using RNA chip (Agilent bioanalyzer). 1 µg of RNA was used to synthesize cDNA (20 µl reaction) (Biorad’s Iscript select cDNA synthesis kit) according to manufacturer’s protocol. The cDNA was diluted 1:10 and used for QPCR with SYBR green detection (Invitrogen). Phusion HF reaction buffer, phusion HF DNA polymerase and dNTPs used for QPCR were purchased from New England Biolabs. Biorad CFX96 Touch real-time PCR detection system was used. Biorad CFX manger software was used to calculate fold changes in gene expression. All primers sequences were listed in supplementary methods . All NMG are defined in supplementary table 3 .
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