B6 nod d17mit21 d17mit10 ltj b6g7

NOD/ShiLtJ (NOD), NOD.CB17-Prkdc^scid/J (NOD-scid), NOD.Cg-Prkd^cscid Tg(CAG-EGFP)10sb/KupwJ (NOD-scid-EGFP), NOD.Cg-Rag1^tm1MomTg(TcraBDC12-4.1) 10JosTg(TcrbBDC12-4.1)82Gse/J (NOD.BDC12-4.1), B6.129S7-Rag1^tm1Mom/J (B6-Rag1^-/-), and B6.NOD-(D17Mit21-D17Mit10)/LtJ (B6^g7) mice were purchased from The Jackson Laboratory. B6.G9C8 mice, transgenic for TCR derived from insulin-specific CD8⁺ T cell clone G9C8, and H-2K^d MHC allele (25 ) were a kind gift by Dr. A. Chervonsky (University of Chicago). B6.NOD-(D17Mit21-D17Mit10)-Rag1^tm1Mom (B6^g7-Rag1^-/-) mice where generated by crossing B6-Rag1^-/- with B6^g7, then intercrossing F1 animals to produce F2 population, which was typed by FACS for presence of T cells in peripheral blood and by PCR for presence of H-2^g7 using 5′-TGCACTTGCATAAGGAAAAC-3′ and 5′-GACTTTGGGGCCTACTTATG-3′ as forward and reverse primers, respectively. Only both H-2^g7-positive and T cells-negative mice were used. To generate bone marrow (BM) chimeric mice B6^g7 or EGFP-positive (NOD × NOD-scid-EGFP) F1 (NOD-EGFP) were used as either BM donors or recipients. All mice were maintained in the Sanford Research Animal Facility according to the NIH guidelines for animal use.