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B6 nod d17mit21 d17mit10 ltj b6g7

Manufactured by Jackson ImmunoResearch

B6.NOD-(D17Mit21-D17Mit10)/LtJ (B6g7) is a laboratory mouse strain. It is a congenic strain derived from the NOD/LtJ (NOD) and C57BL/6J (B6) mouse strains. The core function of this strain is to serve as a research tool for studying genetic loci related to autoimmune diseases.

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3 protocols using b6 nod d17mit21 d17mit10 ltj b6g7

1

Generation of Mouse Strains for Autoimmune Research

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NOD/ShiLtJ (NOD), NOD.CB17-Prkdcscid/J (NOD-scid), NOD.Cg-Prkdcscid Tg(CAG-EGFP)10sb/KupwJ (NOD-scid-EGFP), NOD.Cg-Rag1tm1MomTg(TcraBDC12-4.1) 10JosTg(TcrbBDC12-4.1)82Gse/J (NOD.BDC12-4.1), B6.129S7-Rag1tm1Mom/J (B6-Rag1-/-), and B6.NOD-(D17Mit21-D17Mit10)/LtJ (B6g7) mice were purchased from The Jackson Laboratory. B6.G9C8 mice, transgenic for TCR derived from insulin-specific CD8+ T cell clone G9C8, and H-2Kd MHC allele (25 ) were a kind gift by Dr. A. Chervonsky (University of Chicago). B6.NOD-(D17Mit21-D17Mit10)-Rag1tm1Mom (B6g7-Rag1-/-) mice where generated by crossing B6-Rag1-/- with B6g7, then intercrossing F1 animals to produce F2 population, which was typed by FACS for presence of T cells in peripheral blood and by PCR for presence of H-2g7 using 5′-TGCACTTGCATAAGGAAAAC-3′ and 5′-GACTTTGGGGCCTACTTATG-3′ as forward and reverse primers, respectively. Only both H-2g7-positive and T cells-negative mice were used. To generate bone marrow (BM) chimeric mice B6g7 or EGFP-positive (NOD × NOD-scid-EGFP) F1 (NOD-EGFP) were used as either BM donors or recipients. All mice were maintained in the Sanford Research Animal Facility according to the NIH guidelines for animal use.
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2

Mouse Strains for Diabetes Research

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Female NOD/ShiLtJ (NOD), NOD.CB17-Prkdcscid/J (NOD-scid), B6.NOD-(D17Mit21-D17Mit10)/LtJ (B6g7), and DBA/2J (DBA) mice were from The Jackson Laboratory. B6.G9C8 mice, transgenic for T-cell receptor (TCR) derived from InsB15–23-specific CD8+ T-cell clone G9C8, and H-2Kd MHC allele (19 (link)) were provided by Dr. A. Chervonsky (University of Chicago, Chicago, IL).
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3

Maintenance and Use of NOD, B6, and B6g7 Mice

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NOD/ShiLtJ (NOD), C57BL/6J (B6), and B6.NOD-(D17Mit21-D17Mit10)/LtJ (B6g7) mice were originally obtained from The Jackson Laboratory. Mice were bred and maintained under specific pathogen–free conditions in our facilities. All experiments were approved by the division of Comparative Medicine of Washington University School of Medicine in St. Louis (accreditation number A3381-01).
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