Serum alanine aminotransferase (ALT) levels were measured using an Olympus AU5400 automatic biochemistry analyzer, and the levels of serum cytokines (TNF-α, IL-1β and IL-6), high-mobility group protein B1 (HMGB1) and S1P were measured by enzyme linked-immunosorbant assay (ELISA) as described previously[12 (link)]. Serum C5a and C5 levels were measured with commercial ELISA kits according to the manufacturer’s instructions.
Au5400 automatic biochemistry analyzer
Manufactured by Olympus
Sourced in Japan
The AU5400 automatic biochemistry analyzer is a clinical laboratory instrument designed for the analysis of various biochemical samples. It is capable of performing a wide range of clinical chemistry tests, including but not limited to the measurement of enzymes, proteins, electrolytes, and other analytes. The AU5400 utilizes advanced spectrophotometric technology to provide accurate and reliable results. It is designed for high-throughput operation and can handle a large number of samples efficiently.
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Lab products found in correlation
8 protocols using au5400 automatic biochemistry analyzer
1
Serum Markers of Liver and Inflammation
2
Evaluating Endothelial Cell Integrity and Function
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Lactate dehydrogenase (LDH) is a cytoplasmic cellular enzyme, which can be released to extracellular space when the cell membrane is disrupted by pathological conditions. Measuring the LDH concentration in supernatant of cultured HAECs is therefore a good marker for determination of cell membrane integrity [15 (link)]. Urea transporters, located in endothelial cells, are responsible for transporting extracellular urea into the cell. Vasodilators (nitric oxide (NO) and prostacyclin I-2 (PGI-2)) and vasoconstrictors (endothelin-1 (ET-1)) can be released by ECs to regulate blood pressure and blood flow. In this study, HAECs were co-cultured with 0.8 μg/mL hollow gold nanoshells for 24 h. Then, the cell culture supernatant was centrifuged at 8000×g, 4 °C for 30 min to remove the rest nanoparticles and cell debris. Supernatant LDH and urea were determined using Olympus AU5400 automatic biochemistry analyzer. The concentrations of ET-1, PGI-2, and NO in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) kits (Jiancheng, Nanjing, China), respectively.
3
Venous Blood Sampling and Analysis
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A venous blood sample was collected from the antecubital vein, separated by centrifugation (2000×g at 4°C for 20 min) and frozen at −80°C for batched storage and analysis. Glycosylated hemoglobin was determined by the Cobas B 101 (Roche) system. The remaining biochemical parameters were measured using an Olympus AU5400 automatic biochemistry analyzer (Olympus, Tokyo, Japan).
4
Serum Biomarker Assessment Protocol
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Serum ALT, aspartate aminotransferase (AST), total bilirubin (TB), creatine kinase (CK), serum creatinine (SCr), and blood urea nitrogen (BUN) were measured using an Olympus Au5400 automatic biochemistry analyzer (Olympus, Tokyo, Japan). HBV DNA was detected using a COBAS AmpliPrep/COBAS TaqMan 48 analyzer (Roche Diagnostics, Basel, Switzerland).
5
Longitudinal Liver Function Evaluation
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The patients' epidemiological and clinical data were collected at baseline and 5 years follow-up, including gender, age, Serum ALT, total bilirubin (TBIL), direct bilirubin (DBIL), gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP). The liver function indexes were determined by Olympus Au5400 automatic biochemistry analyzer (Olympus, Tokyo, Japan).
6
Multi-organ Injury Assessment in Stool-Positive Patients
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Fresh stools were collected from both groups immediately after hospitalization. RV antigen detection was performed using the gold labeled method (the kit was supplied by Beijing Wantai Biological Pharmacy, China). Venous blood (approximately 2 mL) was extracted on an empty stomach in the morning. Serum glutamic oxaloacetic aminotransferase (SCOT), creatine kinase-MB (CK-MB), and lactate dehydrogenase (LDH) were continuously determined using an AU5400 automatic biochemistry analyzer (Olympus, Tokyo, Japan). The judgment criteria of parenteral damages were as follows: 1) hepatic damage: SCOT > 40 U/L; 2) myocardial damage: CK-MB > 25 U/L and LDH > 215 U/L; 3) respiratory tract infection: cough, nasal discharge, thickened bilateral pulmonary respiratory sounds, and increased bilateral pulmonary markings according to chest radiography; and 4) central nervous system (CNS) damage: altered consciousness, convulsions, intracranial hypertension, or changes in cerebrospinal fluid.
7
Serum Biomarker Profiling and Histopathology
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Serum samples were obtained by separating the supernatant from the blood after 30 min of coagulation at room temperature. After centrifugation (3000 rpm for 10 min), the serum ALT, AST, total bilirubin (TBIL), direct bilirubin (DBIL), alkaline phosphatase (ALP), and total bile acid (TBA) levels were measured with an Olympus AU5400 automatic biochemistry analyzer (Olympus Optical, Tokyo, Japan). Liver tissues for histopathological examination were fixed and preserved in 10% neutral buffered formalin, processed and trimmed, embedded in paraffin, sectioned to a thickness of approximately 5 μm, and stained with hematoxylin and eosin (HE).
8
Serum Biomarker Analysis Protocol
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Hydroxyproline, ALT, and AST kits were utilized in accordance with the manufacturers’ protocols. Briefly, serum samples were obtained by separating the supernatant from the blood. After centrifugation (3000 rpm for 10 min), serum ALT and AST levels and the hydroxyproline content were measured using an Olympus AU5400 Automatic Biochemistry Analyzer (Olympus Optical, Tokyo, Japan).
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