Cyclin b1 sc 245

Garlic extract was obtained from Egarak (Busan, Korea). Polyclonal antibodies against p-Cdc2 p34 (sc-12340-R), Cdc2 p34 (sc-54), CHK2 (sc-9064), Cdc25c (sc-327), p-Cdc25c (sc12354), p21WAF1 (sc-756), p53 (sc-126), Cyclin A (sc-751), Cyclin B1 (sc-245), p-ATM (sc-47739), ATM (sc-23921), WEE1 (sc-325), HSPA6 (sc-374589) and GAPDH (sc-20357) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Polyclonal antibodies against CHK1 (2360), p-CHK1 (2341), p-CHK2 (2661), ERK (9102), p-ERK (9101), JNK (9258), p-JNK (9251), p38 MAP kinase (9212), p-p38 MAP kinase (9211), AKT (9272), and p-AKT (9271) were obtained from Cell Signaling Technology Inc. (Danvers, MA, USA). Goat anti-rabbit IgG-horseradish peroxidase (HRP) (sc-2004), goat anti-mouse IgG-HRP (sc-2005), and donkey anti-goat IgG-HRP (sc-2020) were purchased from Santa Cruz Biotechnology Inc. Western Lightning Plus-ECL was obtained from PerkinElmer, Inc. (PerkinElmer, MA, USA). U0126, SB203580, SP600125, and wortmannin, were obtained from Calbiochem (San Diego, CA). A Nuclear Extract kit and EMSA Gel Shift kit were obtained from Panomics (Fremont, CA, USA). HSPA6 cDNA was obtained from the Korea human gene bank.

Protease inhibitor cocktail (P8340), phosphatase inhibitor cocktail 2 (P5726) and 3 (P0044), and dimethyl sulfoxide (D8418) were purchased from Sigma-Aldrich. The primary antibodies used in this study from Santa Cruz biotechnology were cyclin D1 (sc-753); cyclin A (sc-596); cyclin B1 (sc-245). Additionally, we used pHH3ser10 (9701A; Cell signaling), LC-3 (L8918; Sigma), cleaved caspase-3, and PARP (cell signaling) and GAPDH (IMG-5019A-2; IMGENEX). Horseradish peroxidase-conjugated secondary antibodies of goat anti-rabbit (4010-05) and goat anti-mouse (1012-05) were purchased from Southern Biotech.

AS was obtained from Dr. Park (Kyungnam University), and AS extraction, separation, and quality control were performed as described previously [22 (link)]. The compound was dissolved in 100% dimethyl sulfoxide (DMSO; Sigma-Aldrich, St. Louis, MO, USA), and a 50 mg/mL stock solution was prepared and stored in small aliquots at −20 °C until needed. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), gelatin, and mitomycin C were purchased from Sigma-Aldrich. A SA-β-gal kit (ab65351) was purchased from Abcam Inc. (Cambridge, MA, UK). Horseradish peroxidase-conjugated anti-mouse (GTX213111-01) and anti-rabbit (GTX213110-01) antibodies were purchased from GeneTex Inc. (Irvine, CA, USA). An NO assay kit (#EMSNO) was purchased from Thermo Fisher Scientific (Vienna, Austria). Phosphorylated (p)-p38 (#9216), p-eNOS (#9570), p53 (#9282), p-AMPK (#2537), p-AKT (#9271), and SIRT1 (#9475) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). β-actin (sc-47778), p-extracellular-signal regulated kinase (ERK) (sc-7383), cdc2 (sc-54), and cyclin B1 (sc-245) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA).