To identify exosome markers, we purchased primary antibodies against CD63 and TSG101 from Abcam (Cambridge, UK), and primary antibodies against Hsp 70 and Hsp 90 were obtained from Cell Signaling Technology (CST, Beverly, MA, USA). The secondary antibodies were F(ab)2 fragments of donkey anti-mouse immunoglobulin or donkey anti-rabbit immunoglobulin linked to horseradish peroxidase (Jackson ImmunoResearch, USA). Immunoblotting reagents from an electrochemiluminescence kit were used (Amersham Biosciences, Uppsala, Sweden).
Primary antibodies against cd63 and tsg101
Manufactured by Abcam
Sourced in United Kingdom
Primary antibodies against CD63 and TSG101 are used to detect the presence and levels of these proteins in biological samples. CD63 is a membrane protein involved in exosome biogenesis, while TSG101 is a protein that plays a role in the formation of multivesicular bodies. These antibodies can be used in various techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of these proteins.
Automatically generated - may contain errors
Lab products found in correlation
Primary antibodies against hsp 70 and hsp 90, by Cell Signaling Technology (3 mentions)
F ab 2 fragments of donkey anti mouse immunoglobulin or donkey anti rabbit immunoglobulin, by Jackson ImmunoResearch (2 mentions)
Horseradish peroxidase, by Jackson ImmunoResearch (2 mentions)
Electrochemiluminescence kit, by Cytiva (1 mentions)
Donkey anti rabbit immunoglobulin, by Jackson ImmunoResearch (1 mentions)
F ab 2 fragments of donkey anti mouse immunoglobulin, by Jackson ImmunoResearch (1 mentions)
Immunoblotting reagents from an electrochemiluminescence kit, by Cytiva (1 mentions)
3 protocols using primary antibodies against cd63 and tsg101
1
Exosome Marker Identification Protocol
2
Exosome Marker Identification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To identify exosome markers, primary antibodies against CD63 and TSG101 were purchased from Abcam (Cambridge, UK), and primary antibodies against Hsp 70 and Hsp 90 were obtained from Cell Signaling Technology (Beverly, MA, USA). The secondary antibodies were F(ab)2 fragments of donkey anti-mouse immunoglobulin or donkey anti-rabbit immunoglobulin linked to horseradish peroxidase (Jackson ImmunoResearch Laboratories, USA). Immunoblotting reagents from an electrochemiluminescence kit were used (Amersham Biosciences, Uppsala, Sweden).
3
Exosome Protein Marker Detection
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To identify exosome markers, primary antibodies against CD63 and TSG101 were purchased from Abcam (Cambridge, UK), and primary antibodies against Hsp 70 and Hsp 90 were obtained from Cell Signaling Technology (CST, Beverly, MA, USA). The secondary antibodies were F(ab)2 fragments of donkey anti-mouse immunoglobulin or donkey anti-rabbit immunoglobulin linked to horseradish peroxidase (Jackson ImmunoResearch, Inc., USA). Immunoblotting reagents from an electrochemiluminescence kit were used (Amersham Biosciences, Uppsala, Sweden).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!