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Pregnenolone sulphate

Manufactured by Merck Group
Sourced in United Kingdom

Pregnenolone sulphate is a laboratory reagent used in various research and analytical applications. It is a sulfated derivative of the steroid pregnenolone, which is a precursor to other steroid hormones. Pregnenolone sulphate serves as a tool for researchers studying steroid biochemistry, neurochemistry, and related fields.

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3 protocols using pregnenolone sulphate

1

Preparation and Storage of GABA, Pregnenolone Sulphate, and Picrotoxinin

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GABA was dissolved in distilled water and stock solutions (1 M) were kept at 4 °C. Pregnenolone sulphate (Sigma) was dissolved in DMSO to a stock concentration of 20 mM and kept at −20 °C. Picrotoxinin (Sigma) was dissolved in DMSO and stored in 100 mM aliquots at −20 °C.
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2

Analytical Characterization of Compounds

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Optical rotations were obtained on a JASCO P200 polarimeter. Melting points were determined using a Büchi® B-540 melting point apparatus. IR spectra were measured on a Perkin-Elmer FTIR Spectrum 2. NMR spectra were recorded on a Bruker Avance III - 500 MHz NMR spectrometer equipped with a multi-nucleus probe BBFO (5 mm). UV spectra were recorded on the PDA detector of the Varian LC-920 system. LC-HRESIMS data were recorded on an Agilent 6520 Accurate-Mass Q-TOF hyphenated to an Agilent 1200 system equipped with a Zorbax Agilent C18 column (50 mm × 2.1 mm, 1.8 μm). Compounds were purified by semi-preparative HPLC with a Gilson 322 system equipped with an Axia C18 column (21.2 mm × 100 mm, 5 μm). HPLC analyses were performed on a Varian LC-920 system with a Kinetex column C18 100 Å (100 × 3.0 mm, 2.6 μm). Solid-phase extractions were performed on Chromabond® SPE cartridges (Macherey-Nagel). The reading of the microplates was performed on an ELISA Versamax plus® (Molecular Devices) plate reader. Solvents for extraction, fractionation and HPLC were supplied by Sigma-Aldrich. The bioassay material was purchased as following: GLP-1 ELISA kit (Active GLP-1, EGLP-35K Millipore), DMEM and PBS (Gibco), BSA (Sigma-Aldrich) and MTS mother solution CellTiter 96 (Promega), compounds standards pregnenolone and pregnenolone sulphate (Sigma-Aldrich).
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3

Pregnenolone Sulphate and MK-801 Electrophysiology

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For all in vitro electrophysiology experiments, Pregnenolone sulphate (Sigma, UK) was prepared as 1 M stock using dimethyl sulfoxide (DMSO). A concentration of 100 µM was used for all electrophysiology experiments. MK-801 (Hello Bio, UK) was prepared with distilled water as 10 mM stock and used at 10 µM concentrations.
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