D glucuronic acid glca

Manufactured by Merck Group

Sourced in United States

D-glucuronic acid (GlcA) is a monosaccharide that serves as a precursor for the synthesis of various biomolecules. It is a key component in the metabolism of carbohydrates and can be utilized in various laboratory applications.

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Glucuronic acid (86 citations) D-glucuronic acid (65 citations) Glucuronic acid (GlcA) (3 citations) D-GlcA (3 citations)

3 protocols using d glucuronic acid glca

Characterization of Pomegranate Peel Compounds

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Compound dexamethasone acetate cream (DXA) was obtained from China Resources Sanjiu Medical and Pharmaceutical Co., Ltd. (Shenzhen, China). Imiquimod cream was purchased from Sichuan Mingxin Pharmaceutical Co., Ltd. (Sichuan, China). P. granatum peels were obtained from fruit purchased from Kangmei Pharmaceutical Co., Ltd. (Guangdong, China). The enhanced chemiluminescence (ECL) reagent was obtained from Millipore (Billerica, MA, USA). d-Fucose (Fuc), d-xylose (Xyl), d-galacturonic acid (GalA), d-galactose (Gal), d-glucose (Glc), d-mannose (Man), l-rhamnose (Rha), d-arabinose (Ara), d-glucuronic acid (GlcA), and d-fructose (Fru) were obtained from Sigma (St. Louis, MO, USA). Deionized water was prepared using a Millipore MilliQ Plus system (Millipore, Bedford, MA, USA). All the other reagents were of analytical grade.

Chen H., Wang C., Tang B., Yu J., Lu Y., Zhang J., Yan Y., Deng H., Han L., Li S, & Lu C. (2022). P. granatum Peel Polysaccharides Ameliorate Imiquimod-Induced Psoriasis-Like Dermatitis in Mice via Suppression of NF-κB and STAT3 Pathways. Frontiers in Pharmacology, 12, 806844.

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Comprehensive Carbohydrate Analysis Protocol

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All foods and food products were purchased from local markets (Davis and Sacramento, CA, USA) including Safeway, Trader Joe’s, Davis Food Co-op, Whole Foods, Nugget Markets, Target, and online (Amazon). Trifluoroacetic acid (TFA, HPLC grade), 3-methyl-1-phenyl-2-pyrazoline-5-one (PMP), chloroform (HPLC grade), ammonium hydroxide solution (NH₄OH) (28–30%), ammonium acetate, sodium acetate, glacial acetic acid, methanol (HPLC grade), D-fructose, D-mannose, D-allose, D-glucose, D-galactose, L-rhamnose, L-fucose, D-ribose, D-xylose, L-arabinose, N-acetyl-D-glucosamine (GlcNAc), N-acetyl-D-galactosamine (GalNAc), D-glucuronic acid (GlcA), and D-galacturonic acid (GalA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Arabinoxylan and polygalacturonic acid were purchased from Megazyme (Bray, Ireland). 96-well Nunc plates and lids were purchased from Thermo Scientific. Viscozyme was provided by Novozyme (Davis, CA, USA). Acetonitrile (ACN) (HPLC grade) was purchased from Honeywell (Muskegon, MI, USA). Nanopure water was used for all experiments.

Castillo J.J., Couture G., Bacalzo NP J.r., Chen Y., Chin E.L., Blecksmith S.E., Bouzid Y.Y., Vainberg Y., Masarweh C., Zhou Q., Smilowitz J.T., German J.B., Mills D.A., Lemay D.G, & Lebrilla C.B. (2022). The Development of the Davis Food Glycopedia—A Glycan Encyclopedia of Food. Nutrients, 14(8), 1639.

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Comprehensive Glycan Analysis Protocol

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Sodium acetate, hydrogen peroxide (H₂O₂) (30% v/v), iron(III)
sulfate pentahydrate (Fe₂(SO₄)₃), trifluoroacetic acid
(TFA) (HPLC grade), chloroform (HPLC grade), ammonium acetate, sodium hydroxide pellets
(semiconductor grade 99.99% trace metals basis), ammonium hydroxide solution
(NH₄OH) (28–30%), 3-methyl-1-phenyl-2-pyrazoline-5-one (PMP),
dichloromethane (DCM), methanol (HPLC grade), anhydrous dimethyl sulfoxide (DMSO), glacial
acetic acid, d-galactose, d-mannose, d-glucose,
d-allose, d-fructose, d-xylose, l-arabinose,
d-xylose, l-fucose, l-rhamnose,
N-acetyl-d-glucosamine (GlcNAc),
N-acetyl-d-galactosamine (GalNAc), d-glucuronic acid
(GlcA), and d-galacturonic acid (GalA) were purchased from Sigma-Aldrich (St.
Louis, MO). Maltotriose, maltotetraose, maltopentaose, and maltohexaose were obtained from
Carbosynth (Compton, UK). Galactan was purchased from Megazyme (Bray, Ireland). C18 and
porous graphitized carbon (PGC) solid phase extraction (SPE) plates were purchased from
Glygen (Columbia, MD). Formic acid (FA) (99.5% optima LC/MS grade) was purchased from
Fisher Scientific (Hampton, NH). Acetonitrile (ACN) (HPLC grade) was purchased from
Honeywell (Muskegon, MI). All purchased chemicals were used without further purification.
Butternut squash was purchased from Pedrick Produce (Dixon, CA). Nanopure water was used
for all experiments.

Castillo J.J., Galermo A.G., Amicucci M.J., Nandita E., Couture G., Bacalzo N., Chen Y, & Lebrilla C.B. (2021). A Multidimensional Mass Spectrometry-Based Workflow for De Novo Structural Elucidation of Oligosaccharides from Polysaccharides. Journal of the American Society for Mass Spectrometry, 32(8), 2175-2185.

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