Cetrimide agar

Manufactured by Merck Group

Sourced in Germany, United States, United Kingdom

Cetrimide agar is a microbiological culture medium used for the selective isolation and identification of Pseudomonas aeruginosa bacteria. It contains cetrimide, a quaternary ammonium compound that inhibits the growth of most other bacteria, allowing Pseudomonas aeruginosa to grow and be easily identified.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Cetrimide (18 citations) P. aeruginosa-specific cetrimide agar (2 citations) Cetrimide fucidin cephaloridine agar (2 citations) Cetrimide Agar (CFC, (2 citations)

50 protocols using cetrimide agar

Microbial Quality Evaluation of Processed Fish

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The evaluation of microbial quality deterioration of gutted and filleted fish samples was based on enumeration of total viable count (TVC), Pseudomonas spp., Enterobacteriaceae spp., and H₂S-producing bacteria (i.e., Shewanella spp.) following the method described by Katsouli et al. [18 (link)]. TVC was grown on plate count agar (PCA, Merck, Darmstadt, Germany), Pseudomonas spp. on Cetrimide agar (CFC, Merck, Darmstadt, Germany), whereas H₂S-producing bacteria were grown on Iron agar (Iron agar, Merck, Darmstadt, Germany); their colonies were enumerated after incubation at 25 °C for 72 h, 48 h, and 48 h respectively. Enterobacteriaceae spp. was grown on violet red bile glucose agar (VRBG, Merck, Darmstadt, Germany) and incubated at 37 °C for 24 h.
The changes of the gas headspace (CO₂, O₂) into the packaging during storage were determined using the CheckMate 9900 O₂/CO₂ meter (PBI Dansensor, Rinsted, Denmark).

Nanou E., Kotsiri M., Kogiannou D., Katsouli M, & Grigorakis K. (2023). Consumer Perception of Freshness and Volatile Composition of Fresh Gilthead Seabream and Seabass in Active Packaging with and without CO2-Emitting Pads. Foods, 12(3), 505.

+ Open protocol

+ Expand

Pseudomonas aeruginosa Nosocomial Infections

Check if the same lab product or an alternative is used in the 5 most similar protocols

Over a 6-month period (October 2020–March 2021), 115 NIs-causing P. aeruginosa isolates were collected from hospitalized patients referred to teaching hospitals of three cities of Khuzestan province (Ahvaz, Abadan and Khorramshahr), southwest Iran. NIs were defined according to the diagnostic criteria of the Centers for Disease Control and Prevention (CDC) [15 ]. The isolates were related to four types of NIs including UTI, VAP, SSI and BSI, which were collected from different wards including intensive care unit (ICU), burn, infectious diseases, nephrology, emergency, pediatrics, gastroenterology, skin diseases, dialysis, internal medicine, and critical care unit (CCU). Initial identification of the isolates was based on typical growth on a selective medium Cetrimide agar (Merck, Darmstadt, Germany) and different biochemical tests including colony characteristics, sulphur indole and motility (SIM), triple sugar iron (TSI), urease, citrate, oxidase, and growth at 42 °C [16 (link)]. PCR of the gyrB gene was used for final identification [17 ]. P. aeruginosa ATCC® 27,853™ was used as a positive control. A stock of all bacterial isolates was prepared in a microtube containing tryptic soy broth (TSB) with 20% glycerol and stored at −80 °C until further investigation.

Heidari R., Farajzadeh Sheikh A., Hashemzadeh M., Farshadzadeh Z., Salmanzadeh S, & Saki M. (2022). Antibiotic resistance, biofilm production ability and genetic diversity of carbapenem-resistant Pseudomonas aeruginosa strains isolated from nosocomial infections in southwestern Iran. Molecular Biology Reports, 49(5), 3811-3822.

+ Open protocol

+ Expand

Antibiotic Susceptibility Testing Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

The present study provided the disks and powdered antibiotics from the MAST (Mast Diagnostics, United Kingdom) and Sigma-Aldrich (Taufkirchen, Germany). The following items were acquired from Merck (Merck, United States): Blood Agar, Mannitol Salt Agar (MSA), MacConkey agar, Cetrimide agar, Mueller Hinton Agar (MHA), DNA Agar, Mueller Hinton Broth (MHB), Trypticase Soy Broth (TSB), NaCl, glucose, and MgCl2. Fetal bovine serum (FBS), Dulbecco’s Modified Eagle’s Medium (DMEM), Fetal-Calf Serum (FCS), 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2 H-tetrazoliumbromide (MTT), Triton X-100, dimethyl sulfoxide (DMSO), agarose, ethanol, methanol, and crystal violet were provided from Sigma-Aldrich (Saint Louis, MO, United States). 96-well microplates, including flat-and round-bottom, were supplied by Jet Biofil (Guangzhou, China) and NEST Biotechnology (Wuxi, China), respectively.

Mirzaei R., Esmaeili Gouvarchin Ghaleh H, & Ranjbar R. (2023). Antibiofilm effect of melittin alone and in combination with conventional antibiotics toward strong biofilm of MDR-MRSA and -Pseudomonas aeruginosa. Frontiers in Microbiology, 14, 1030401.

+ Open protocol

+ Expand

Isolation and Identification of P. aeruginosa

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sixty isolates of P. aeruginosa were collected from two hospitals in Kermanshah (Imam Khomeini [A] and Imam Reza [B]) between 2011 and 2012. The specimens were collected from burns (n = 32, 53.3%), urine (n = 9, 15%), respiratory tract secretions (n = 10, 16.7%), and other sources (vagina, catheter, eye, ear, wound, and blood) (n = 9, 15%). The bacteria were then cultured on the MacConkey agar and the Muller-Hinton agar media, and the grown colonies were confirmed using specific tests. These included catalase, oxidase, the growth characteristics and pigment gas production, indole and methyl red, Sulfide Indole Motility, and growth at 42°C on the Cetrimide agar (Merck, Germany).

Akya A., Salimi A., Nomanpour B, & Ahmadi K. (2015). Prevalence and Clonal Dissemination of Metallo-Beta-Lactamase-Producing Pseudomonas aeruginosa in Kermanshah. Jundishapur Journal of Microbiology, 8(7), e20980.

+ Open protocol

+ Expand

Exclusion of P. aeruginosa by L. acidophilus

Check if the same lab product or an alternative is used in the 5 most similar protocols

The exclusion effect of L. acidophilus on P. aeruginosa adhesion to A549 monolayers was also evaluated using CFU counts. To this end, un-labeled lactobacilli (L. acidophilus and L. rhamnosus) and P. aeruginosa (CF1 and CF4) were used in exclusion assays as described above. Following the incubation with P. aeruginosa, cells were washed to remove un-bound bacteria and lysed by adding 0.1% Triton X-100 solution for 10 min. Following a wash with PBS at 4000× g for 5 min, bacteria were resuspended in PBS, serially diluted, and plated onto cetrimide agar (Merck) to determine CFU counts.

Batoni G., Kaya E., Catelli E., Quinti S., Botti M., De Carli A., Bianchi M., Maisetta G, & Esin S. (2023). Lactobacillus Probiotic Strains Differ in Their Ability to Adhere to Human Lung Epithelial Cells and to Prevent Adhesion of Clinical Isolates of Pseudomonas aeruginosa from Cystic Fibrosis Lung. Microorganisms, 11(7), 1707.

+ Open protocol

+ Expand

Cultivation and Characterization of Pseudomonas aeruginosa Strains

Check if the same lab product or an alternative is used in the 5 most similar protocols

Reference P. aeruginosa strains PAO1, PA14, and PAK and 11 clinical P. aeruginosa isolates were used in this study. Clinical strains were selected from the CF Bacterial Repository at the National Heart and Lung Institute, Imperial College London; this collection consists of bacteria isolated from airway samples of people with cystic fibrosis at the Royal Brompton Hospital, London (Table 1). Bacteria were stored in Microbank vials (Pro-Lab Diagnostics) at −80°C. Clinical strains were selected with a range of antibiograms according to clinical lab results using EUCAST clinical breakpoints. Isolates were revived onto cetrimide agar (Merck) for confirmation of purity and grown overnight at 37°C followed by subculture onto LB agar (Merck). Single colonies were inoculated into Mueller-Hinton broth (Merck) and incubated overnight at 37°C with agitation at 200 rpm.

Murphy R.A., Coates M., Thrane S., Sabnis A., Harrison J., Schelenz S., Edwards A.M., Vorup-Jensen T, & Davies J.C. (2022). Synergistic Activity of Repurposed Peptide Drug Glatiramer Acetate with Tobramycin against Cystic Fibrosis Pseudomonas aeruginosa. Microbiology Spectrum, 10(4), e00813-22.

+ Open protocol

+ Expand

Carbapenem-resistant Pseudomonas aeruginosa Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Effluents from both WWTPs were sampled monthly for one-year period. Effluent water was refrigerated until further processed in the laboratory, usually within 6 hours of collection.
For selective isolation of carbapenem-resistant P. aeruginosa 100 ml of effluent water was filtered through 0.45 μm filter (Whatman). Filters were plated onto in-house selective medium (cetrimide agar (Merck)) supplemented with imipenem with a final concentration of 4 μg/mL) and incubated at 42°C for 48-hours.
After incubation up to ten suspected P. aeruginosa colonies were subcultured and identified by MALDI-TOF. All isolates confirmed as P. aeruginosa were submitted for identical antibiotic susceptibility testing as described above for clinical strains.
Isolates of P. aeruginosa resistant to either imipenem or meropenem or both were stored at -70°C for further processing.

Golle A., Janezic S, & Rupnik M. (2017). Low overlap between carbapenem resistant Pseudomonas aeruginosa genotypes isolated from hospitalized patients and wastewater treatment plants. PLoS ONE, 12(10), e0186736.

+ Open protocol

+ Expand

Microbial Enumeration in Food Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

For total viable count (ISO 4833-2, 2013 ),
a 1 mL sample was taken from tubes containing the mixed sample, and 10-fold
dilutions were performed with 0.1% BPW. The 0.1 mL of the diluted sample
was shifted to plates containing nutrient agar (1.05450, Merck KGaA, Darmstadt,
Germany). The visible colonies were counted using the JP Selecta Digital S
colony counter (4905002, Spain). For the total coliform count (ISO 4832, 2006 ), a 1 mL sample was diluted
as mentioned above. A diluted sample, 0.1 mL, was transferred by a pipette and
uniformly spread on MacConkey agar (Merck KGaA, 1.05465). Pink colonies appear
on the agar that was counted through the colony counter. For
Pseudomonas count (ISO
13720, 2010), 0.1 mL from the diluted sample was shifted and spread
on Cetrimide agar (1.05284, Merck KGaA) as per protocol. The Green water-soluble
pigment of both colonies and the media was an indication of the presence of
Pseudomonas. Plates were incubated for 48 h at 37°C.
The visible colonies were counted using the colony counter. For
Salmonella count (ISO 6579,
2002), the sample was diluted, and 0.1 mL for the diluted sample was
transferred to Salmonella shigella (SS) agar (1.07667, Merck
KGaA) and is spread uniformly on the plates. Colorless to black-centered
colonies appeared, indicating the presence of Salmonella.

Nauman K., Jaspal M.H., Asghar B., Manzoor A., Akhtar K.H., Ali U., Ali S., Nasir J., Sohaib M, & Badar I.H. (2022). Effect of Different Packaging Atmosphere on Microbiological Shelf Life, Physicochemical Attributes, and Sensory Characteristics of Chilled Poultry Fillets. Food Science of Animal Resources, 42(1), 153-174.

+ Open protocol

+ Expand

Pseudomonas aeruginosa Subculture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Subcultures of P. aeruginosa ATCC 15442, P. aeruginosa PAO-1, and P. aeruginosa ATCC 27853 were used from Hamadan Medical University, Microbiology Department microbial bank. Reference strains were cultivated at 37 °C for 24 h in cetrimide agar (Merck, Germany). All strains were kept at −20 °C in TSB containing 20% glycerol. The Ethics Committee approved this study of Hamadan University of Medical Sciences (Code No: IR.UMSHA.REC.1398.573).

Dehbashi S., Tahmasebi H., Alikhani M.Y., Keramat F, & Arabestani M.R. (2022). Optimization and development of high-resolution melting curve analysis (HRMA) assay for detection of New Delhi metallo-β-lactamase (NDM) producing Pseudomonas aeruginosa. AIMS Microbiology, 8(2), 178-192.

+ Open protocol

+ Expand

Pseudomonas Identification from Burn Wounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

For sampling, wound swabs were obtained from the burn patients and P. aeruginosa was identified by standard microbiological methods including Gram stains, culture on mediums such as blood agar, MacConkey agar, triple sugar iron agar (TSI), cetrimide agar (Merck, UK), oxidase (Sigma, USA), catalase, oxidative/fermentative (OF), indole, methyl red (MR), Voges-Proskauer (VP) tests (Merck, UK), and growth at 42°C.

Saffari M., Firoozeh F., Pourbabaee M, & Zibaei M. (2016). Evaluation of Metallo-β-Lactamase-Production and Carriage of bla-VIM Genes in Pseudomonas aeruginosa Isolated from Burn Wound Infections in Isfahan. Archives of Trauma Research, 5(4), e34343.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!