Sb415286

Manufactured by Abcam

SB415286 is a specific and potent inhibitor of the p38 mitogen-activated protein kinase (MAPK) signaling pathway. It acts by blocking the catalytic activity of p38 MAPK enzyme. The compound is commonly used as a research tool in cell biology and biochemistry studies to investigate the role of p38 MAPK in various cellular processes.

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Lab products found in correlation

Mk 801, by Merck Group (1 mentions) Spermine, by Bio-Techne (1 mentions) Pd0325901, by Bio-Techne (1 mentions) Mk 801, by Bio-Techne (1 mentions) Sb202190, by Bio-Techne (1 mentions) Kt5720, by Abcam (1 mentions) Fura 2 am, by Merck Group (1 mentions) Sp600125, by Abcam (1 mentions) Sb203580, by Bio-Techne (1 mentions) Wortmannin, by Abcam (1 mentions) Anti neun, by Merck Group (1 mentions) Anti creb, by Santa Cruz Biotechnology (1 mentions) Ruthenium red, by Abcam (1 mentions) Anti map2, by Merck Group (1 mentions) Kn 93, by Abcam (1 mentions) D apv, by Bio-Techne (1 mentions) Anti p38 mapk, by Cell Signaling Technology (1 mentions) Pd98059, by Abcam (1 mentions) Thapsigargin, by Abcam (1 mentions)

2 protocols using sb415286

Patch-Clamp Electrophysiology with GSK3β Inhibitor

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In some patch-clamp experiments, MK-801 (200 μM; purchased from Sigma-Aldrich) and SB415286 (10 μM in internal solution + 0.01% of the vehicle DMSO; purchased from Abcam) were additionally included in the patch-pipette. The inhibitor and control internal solutions were given an initial, ~30 min, equilibration time. Half of the experiments were blinded. Up state spike rates were relatively low (0.22 ± 0.08, n = 23). It needs to be mentioned that there are two GSK isoforms (GSK3α and GSK3β) expressed in the mammalian brain. However, since the GSK3β isoform is generally considered to be the critical one in regulating synaptic plasticity^{56 (link)}, we refer throughout this study to GSK3β as the main target of SB415286.

Bartram J., Kahn M.C., Tuohy S., Paulsen O., Wilson T, & Mann E.O. (2017). Cortical Up states induce the selective weakening of subthreshold synaptic inputs. Nature Communications, 8, 665.

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Neuronal Signaling Pathway Analysis

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Anti CREB pS133 (1:1000; Millipore); anti-CREB (1:500; Santa Cruz Biotechnology); anti P38 MAPK pThr180/182 (1:1000; Cell signal); anti P38 MAPK (1:1000; Cell signal); anti MAP2 (1:500; Millipore); anti NeuN (1:500; Millipore). Chemicals from Sigma: NMDA, DAPV, spermine, PD0325901; Chemicals from Tocris: MK801, SB202190, SB203580, TTX, TEA, KN93, STO-609, LY292004, KT5720, SB415286, PD98059, SP600125, Wortmannin, Dantrolene, Thapsigargin, Ruthenium Red, Naltriben; Imipramine was from Abcam; Cyclopiazonic acid was from MP Biomedicals. Magnesium Green™ AM and Fura-2 AM were from Millipore.

Hou H., Wang L., Fu T., Papasergi M., Yule D.I, & Xia H. (2020). Magnesium acts as a second messenger in the regulation of NMDA receptor mediated CREB signaling in neurons. Molecular neurobiology, 57(6), 2539-2550.

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