5431 autoanalyzer

D-dimer, TnI, N-terminal pro-B-type natriuretic peptide (NT-proBNP), C-reactive protein (CRP) were measured as cardiac biomarkers. D-dimer was measured with immunoturbidimetric assay using CA-7000 system (Sysmex, Kobe, Japan), and the reference value is < 0.5 mg/L. TnI was measured using electrochemiluminescence sandwich immunoassay method using Dimension RxL-Max (Siemens), and the reference value is < 0.05 ng/mL. NT-proBNP was measured using an electrochemiluminescence sandwich immunoassay method for NT-proBNP with an Elecsys 2010 analyzer (Roche Diagnostics, Mannheim, Germany). The analytic range of the NT-proBNP assay extends from 5 to 35,000 pg/mL. The reference value varies according to age and gender, and < 88 pg/mL for men and < 153 pg/mL for women in our institution. High-sensitivity C-reactive protein (hsCRP) was measured by the immunoturbidimetric CRP-Latex (II) assay using an Olympus 5431 autoanalyzer (Olympus America Inc., Center Valley, PA, USA) with reference range of < 0.5 mg/dL.

Routine laboratory studies were obtained at the time of hospital admission. Cardiac enzymes, including CK-MB and Tn-I, were checked serially, and the maximal values were used in our data analysis. Blood samples to assess the serum lipid profile and glucose levels were obtained on the morning following admission. High-sensitivity C-reactive protein (hs-CRP) was measured by means of the immunoturbidimetric CRP-Latex (II) high-sensitive assay using an Olympus 5431 autoanalyzer (Olympus America Inc., Melville, NY, USA). Serum N-terminal pro-B-type natriuretic peptide (NT-proBNP) was measured using an electrochemiluminescence sandwich immunoassay method with an Elecsys 2010 analyzer (Roche Diagnostics, Mannheim, Germany), having an analytic range that extended from 5 to 35000 pg/mL.15) (link)