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2 protocols using anti pdk4 antibody

1

Molecular Signaling Pathway Analysis

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Anti-phospho-SRC, anti-SRC, anti-phospho-ERK, anti-ERK, anti-phospho-JNK, and anti-JNK antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-PDK4 antibody was purchased from Novus Biologicals (Littleton, CO, USA). Dimethyl sulfoxide (DMSO), crystal violet, a cell migration kit, and a cell invasion kit were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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2

Immunoblot analysis of cellular pathways

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All chemicals used in this study were purchased from Sigma-Aldrich unless otherwise stated. The rabbit polyoclonal anti-α-tubulin FITC-conjugated antibodies, the anti-p-mTOR, anti-mTOR, ani-Sirt1, anti-FOXO3a, anti-LC I/II, anti-p-AKT, anti-AKT, anti-p-ERK1/2 and anti-BCL2 antibodies were from Cell Signaling Technology (Beverly MA, USA). The rabbit polyclonal anti-PDK4 antibody was from Novus (Dallas, TA, USA). The mouse monoclonal anti-ACTIN antibody was from Sigma (St. Louis, MO, USA).
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