PCR assays were conducted using Premix Taq (Ex Taq II) (Takara) according to the manufacturer’s instructions. The PCR products were then submitted to agarose gel electrophoresis in 2% agarose with TAE buffer by using an electrophoresis system (BIO-RAD) and visualized by using an imaging system (BIO-RAD).
Premix taq ex taq 2
Manufactured by Takara Bio
Premix Taq (Ex Taq II) is a ready-to-use master mix for PCR amplification. It contains Taq DNA polymerase, dNTPs, and reaction buffer optimized for efficient DNA amplification.
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2 protocols using premix taq ex taq 2
1
PCR Assays Using Premix Taq
2
CircRNA Expression Analysis by qPCR
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The expression levels of the circRNAs were analyzed by PCR using Premix Taq (Ex Taq II) (Takara) in 20 μl reaction volume, including 1 μl cDNA, 10 μl 2 × master mix, 0.3 μl forward primer (10 μM), 0.3 μl reverse primer (10 μM) and 8.4 μl double distilled water. The reaction was set at 95°C for 10 min, repeated for 40 cycles at 95°C for 20 s, 60°C for 30 s, 72°C for 45 s and finally 72°C for 7 min in PCR System (BIO-RAD, CA, USA).
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