Anti rictor antibody

Manufactured by Cell Signaling Technology

The Anti-Rictor antibody is a laboratory research tool that specifically binds and detects the Rictor protein. Rictor is a key component of the mTOR complex 2 (mTORC2), which plays a critical role in regulating cellular processes such as cell growth, proliferation, and metabolism. This antibody can be used in various analytical techniques, including Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of Rictor in biological samples.

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Lab products found in correlation

Anti tbk1 antibody, by Cell Signaling Technology (1 mentions) Anti ha antibody, by Cell Signaling Technology (1 mentions) Anti α tubulin antibody t5168, by Merck Group (1 mentions) Anti myc tag antibody, by Cell Signaling Technology (1 mentions) Anti sting antibody, by Cell Signaling Technology (1 mentions) Anti irf3 antibody, by Cell Signaling Technology (1 mentions) Anti irf7 antibody, by Cell Signaling Technology (1 mentions) Anti vinculin antibody sc 25336, by Santa Cruz Biotechnology (1 mentions) Anti rbx1, by Cell Signaling Technology (1 mentions) Anti c myc antibody, by Cell Signaling Technology (1 mentions)

2 protocols using anti rictor antibody

Comprehensive Antibody Optimization Protocol

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All antibodies were used at a 1:1,000 dilution in TBS with 0.1% Tween 20 detergent buffer with 5% non-fat milk for Western blotting. Anti-Myc-Tag antibody (2278), anti-HA antibody (3724), anti-Phospho-Akt Substrate (RXRXXpS*/pT*) antibody (10001), anti-p-AKT (Ser473; 4060), anti- anti-p-p70 S6 Kinase (Thr389; 9234), anti-p-S6 Ribosomal Protein (Ser240/244; 5364), anti-p-4EBP1 (Thr37/46; 2855), pIRF3 antibody (Ser386; 37829), anti-IRF3 antibody (4302), anti-IRF7 antibody (4920), anti- anti-p-TBK1 (Ser172; 5483), anti-TBK1 antibody (51872), anti-STING antibody (13647), anti-c-Myc antibody (18583), anti-RSK1/2/3 antibody (9347), anti-Rictor antibody (9476), anti-Rbx1 (11922), anti-Skp1 antibody (12248), anti-rabbit IgG, HRP-linked antibody (7074), and anti-mouse IgG, HRP-linked antibody (7076) were obtained from Cell Signaling Technology. Anti-cyclin E antibody (sc-198), anti-β-catenin antibody (sc-59737), anti-c-Jun antibody (sc-45), anti-GST antibody (sc-459), anti-Cul1 (sc-11384), and anti-vinculin antibody (sc-25336) were obtained from Santa Cruz Biotechnology. Polyclonal anti-Flag antibody (F-7425), monoclonal anti-Flag antibody (F-3165, clone M2), and anti-α-tubulin antibody (T-5168) were obtained from Sigma-Aldrich. Anti-BUD13 antibody (20163-1-AP) and anti-Fbw7 antibody (28424-1-AP) were obtained from Proteintech.

Chen J., Zhang X., Tan X, & Liu P. (2023). Somatic gain-of-function mutations in BUD13 promote oncogenesis by disrupting Fbw7 function. The Journal of Experimental Medicine, 220(10), e20222056.

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In vitro Kinase Assay for mTORC1 and mTORC2

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The in vitro kinase assays for mTORC1 and mTORC2 activity were performed according to the protocol adopted from Dr. David Sabatini’s laboratory. Briefly, cells were lysed in 200 μL of lysis buffer containing 40 mm HEPES, pH 7.5, 120 mM NaCl, 0.3% CHAPS, 1 mM EDTA, 2.5 mM sodium pyrophosphate. Cell lysate was passed through a sepharose-affinity column conjugated with anti-raptor or anti-Rictor antibody (Cell Signaling, CA). After appropriate washing with a kinase buffer containing 1 mM dithiothreitol, beads were incubated for 30 min at 30 °C with purified recombinant S6K and AKT as substrate for mTORC1 and mTORC2 activity, respectively. The reactions were then terminated by boiling in the presence of 1× SDS sample buffer. Phosphorylation was detected by western blotting using anti-pp70S6K antibody for mTORC1 activity and antiphosphorylated AKT (S473) antibody for mTORC2 activity.

Dhar S.K., Bakthavatchalu V., Dhar B., Chen J., Tadahide I., Zhu H., Gao T, & St. Clair D.K. (2018). DNA polymerase gamma (Polγ) deficiency triggers a selective mTORC2 prosurvival autophagy response via mitochondria-mediated ROS signaling. Oncogene, 37(48), 6225-6242.

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