Pseudomonas aeruginosa

Selected bacteria (B. cereus (DSM 345), E. coli (DSM 498), Pseudomonas aeruginosa (DSM 1128), S. aureus (DSM 346)) and fungi (black mold Aspergillus brasiliensis (DSM 1988), yeast Candida albicans (DSM 1386)) were purchased from DSMZ-German Collection of Microorganisms and Cell Cultures GmbH from Berlin, Germany.

Pseudomonas aeruginosa (DSM no. 939) was obtained from the Leibniz-Institute DSMZ- German Collection of Microorganisms and Cell Cultures. Methicillin-resistance Staphylococcus aureus (MRSA) was a clinical isolate, kindly provided by B. Ghebremedhin (Helios university medical centre, Wuppertal, Germany). Both strains were cultured on casein/soy peptone agar plates (CSA; pH 7.2) containing 15 g/l casein peptone, 5 g/l soy peptone, 5 g/l sodium chloride and 15 g/l agar (AppliChem, Darmstadt, Germany) under aerobic conditions at 37 °C.

Gentamicin sulfate (solubilized in water, 3%, GS, Sigma-Aldrich, St. Louis, MO, USA) was used as an antibiotic. Tetraethylorthosilicate (TEOS, 98%, Aldrich, St. Louis, MO, USA) was used as the silica particle precursor). Octyltriethoxysilane (OTES, 97%, Fluka, Philadelphia, PA, USA) was used as the modifying agent. Isopropyl alcohol (iPrOH, 99.9%, Chimreactiv S.R.L., Bucharest, Romania) was used as the solvent. Aqueous solution of ammonia (NH₄OH, 25%, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) was used as the catalyst. The chemicals were used as received. The glass substrate was purchased from FabTech (Bucharest, Romania) and was chosen in order to investigate the topography of films obtained by deposition of final materials.
Three fungal strains, Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli), purchased from German Collection of Microorganisms and Cell Cultures (DSMZ) (Braunschweig, Germany), were used in the experiments.