1587 cercopithecus aethiops

Cell lines were purchased from the American Type Culture Collection (ATCC). The absence of mycoplasma contamination was checked periodically by the Hoechst staining method. Cell lines supporting the multiplication of Coronaviruses were the following: Monkey kidney (Vero-76) [ATCC CRL 1587 Cercopithecus Aethiops], Monkey kidney (Vero C1008, clone E6) [ATCC CRL 1586 Cercopithecus Aethiops].
Human coronaviruses were: (i) Coronaviridae: Betacoronavirus strain OC43 (ATCC VR-1558), Alphacoronavirus strain 229E (ATCC VR-740), Betacoronavirus SARS-CoV-2 (strain VR PV10734) clinical isolate, kindly provided by Lazzaro Spallanzani Hospital, Rome, Italy. All experimental steps involving the SARS-CoV-2 virus were performed in a biosafety level 3 (BSL3) containment laboratory.

Cell lines were purchased from American Type Culture Collection (ATCC). Cell lines supporting the multiplication of CV-B5 and Sb-1 are Monkey kidney (Vero-76) [ATCC CRL 1587 Cercopithecus Aethiops]. Viruses were purchased from the American Type Culture Collection (ATCC). Picornaviridae: Coxsackievirus [coxsackie type B5 (CV-B5), strain Faulkner (ATCC VR-185)], and human enterovirus C [poliovirus type-1 (Sb-1), Sabin strain Chat (ATCC VR-1562)]. Cell cultures were checked periodically for the absence of mycoplasma contamination with MycoTect Kit (Gibco). Viruses were maintained in our laboratory and propagated in appropriate cell lines. The viruses were stored in small aliquots at −80 °C until use.