Dynamic light scattering measurements were performed on a Malvern Zetasizer Nano ZS90 DLS at an angle of 175°. To prepare samples, lyophilized I40 was dissolved on ice for at least 10 min using pre-chilled, sterile-filtered deionized water to a final concentration of 12.5 μM. Measurements were made in a clean 1 cm polystyrene cell. Three acquisitions were performed at each temperature, with each acquisition taking 2–3 min. The temperature of the sample was increased from 10–30 °C in increments of 2 °C with a 3 min equilibration at each temperature before acquisition. Peak sizes were assigned based on intensity analysis while relative proportions of different peaks were assigned based on volume distribution analysis.
Zetasizer nano zs90 dls
Manufactured by Malvern Panalytical
Sourced in United Kingdom
The Zetasizer Nano ZS90 is a dynamic light scattering (DLS) instrument used for the measurement of particle size, zeta potential, and molecular weight. It utilizes a 4 mW He-Ne laser operating at a wavelength of 633 nm and a non-invasive backscatter (NIBS) detection technology to analyze the Brownian motion of particles or molecules in a sample.
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Lab products found in correlation
Su8010, by Hitachi (2 mentions)
Ht7700, by Hitachi (1 mentions)
Em ace600, by Leica (1 mentions)
Jem 1230, by JEOL (1 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
Human serum albumin (hsa), by Thermo Fisher Scientific (1 mentions)
Fluoromax 4 spectrofluorometer, by Horiba (1 mentions)
6 protocols using zetasizer nano zs90 dls
1
Dynamic Light Scattering of I40 Protein
2
Particle Size and Zeta Potential of Nanoplexes
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Average particle size and zeta potential of the as-prepared PMA6Chol-r-PDMAEMA/pDNA and PMA6Dios-r-PDMAEMA/pDNA nanoplexes under various N/P ratios were analyzed on a Malvern Zetasizer Nano ZS90 DLS instrument at 25 °C with incident laser beams at λ = 633 nm and a scattering angle of 90°.
3
UV-induced Area Change in LUVs
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The average diameter of the LUVs was measured through Zetasizer Nano ZS90 DLS (Malvern Instruments, Malvern, United Kingdom) equipped with a 632.8 nm 4 mW HeNe laser and measuring the scattered light at 173°. In order to detect the UV‐induced area change of the LUVs, the UV LED (used also for the measurements with GUVs) was mounted inside the measuring compartment of the Zetasizer. Three replicates were produced for each light condition for each sample. The average size distribution of the LUVs was plotted and the area of LUVs was calculated from the formula of a sphere, 4πR2, in which R is the LUV radius. Area increase is defined similarly to that for the GUV measurements, namely as A L − A i A i × 100
4
Characterization of Nanocapsule Morphology
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A small portion of the solid powder was diluted in deionized water to obtain a transparent suspension. The mean particle size (MPS) and polydispersity index (PDI) of the nanocapsules in the transparent solution were measured by dynamic light scattering (DLS Zetasizer Nano ZS90, Malvern Instruments, Worcestershire, UK). Each sample was measured in parallel three times, and the mean values and standard deviation were calculated.
A scanning electron microscope (SEM, HITACHI, Su-8010, Tokyo, Japan) was used to characterize the morphology of the nanocapsules. An aliquot of the resuspended nanocapsules was dropped onto the surface of a cleaned silicon water. The SEM images were acquired at an acceleration voltage of 5 kV. A transmission electron microscope (TEM, HITACHI, HT7700, Tokyo, Japan) was used to characterize the internal structure of the prepared nanocapsules. An appropriate volume of the transparent solution was dropped onto 300-mesh copper grid coated with a carbon film. TEM images were acquired at an acceleration voltage of 80 kV.
A scanning electron microscope (SEM, HITACHI, Su-8010, Tokyo, Japan) was used to characterize the morphology of the nanocapsules. An aliquot of the resuspended nanocapsules was dropped onto the surface of a cleaned silicon water. The SEM images were acquired at an acceleration voltage of 5 kV. A transmission electron microscope (TEM, HITACHI, HT7700, Tokyo, Japan) was used to characterize the internal structure of the prepared nanocapsules. An appropriate volume of the transparent solution was dropped onto 300-mesh copper grid coated with a carbon film. TEM images were acquired at an acceleration voltage of 80 kV.
5
Morphological Analysis of PLGA Microcapsules
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The morphology of the PLGA microcapsules was observed using scanning electron microscopy. The freeze-dried microcapsules were suspended in water and dropped onto a silicon wafer and dried overnight. After coating with a thin layer of platinum using a sputtercoater (EM ACE600, Leica, Vienna, Austria), the samples were characterized by scanning electron microscope (SEM, HITACHI, Su-8010, Tokyo, Japan). The accelerating voltage used was 10.0 kV, and the current was 5.0 µA. A laser particle size analyzer (DLS Zetasizer Nano ZS90, Malvern Instruments, Worcestershire, UK) was used to measure the particle size and polydispersity index of the microcapsules at room temperature. PLGA microcapsules were diluted to a concentration of 1 µg/mL using distilled water. The final values were the average of three measurements.
6
Synthesis and Characterization of HSA@Cy-HPT Nanoparticles
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2.1 Preparation and characterization of HSA@Cy-HPT HSA@Cy-HPT was prepared using a method similar to the one previously reported. 48 Briefly, 5 mg of as-synthesized Cy-HPT was firstly dispersed in 10 mL methanol. HSA (Sigma-Aldrich, 200 mg) and 10 mL TEA (triethylamine) were mixed in 10 mL of ultrapure water for 30 min. Then, the mixture was added to 10 mL of the above-mentioned methanol solution of Cy-HPT for vigorous stirring overnight. After a 24 h dialysis of the reaction solution with a dialysis bag (MWCO 10 kDa), HSA@Cy-HPT nanoparticles were obtained. The mean particle size was determined by DLS Zetasizer Nano ZS90 (Malvern Instruments Ltd, UK) and TEM (JEOL, model JEM-1230, Japan), operated at 100 kV. Optical properties of chemicals and HSA@Cy-HPT nanoparticles were characterized using Thermo Scientific NanoDrop 2000/2000C spectrophotometer and a NIR fluorescence spectrometer (HORIBA Scientific Fluoromax-4 spectro fluorometer with a xenon lamp and 1.0 cm quartz cell) with 810 nm excitation and scanning wavelength from 830 nm to 900 nm (5 nm slit width at a scan rate of 600 nm min À1 ).
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