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μmanager

Manufactured by Molecular Devices
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μmanager is a versatile open-source microscope control software that provides a standardized interface for controlling a wide range of microscope hardware components and devices. It supports a comprehensive range of microscopes, cameras, stages, and other peripherals, allowing users to integrate and automate their imaging workflows.

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Lab products found in correlation

Inverted epifluorescence microscope, by Olympus (2 mentions) Metamorph, by Molecular Devices (2 mentions)

2 protocols using μmanager

1

Donor-decay Kinetics Imaging Protocol

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Donor-decay time-course was studied as before (Plant et al., 2010 (link)), using an Olympus inverted epi-fluorescence microscope. Cells were studied in a solution comprising (in mM): NaCl 130, KCl 4, MgCl2 1.2, CaCl2 2, HEPES 10, pH was adjusted to 7.4 with NaOH. mTFP1 was excited at 445 nm and the emission collected through a 470–500 nm bandpass filter, YFP was excited at 514 nm and the emission collected through a 525–575 nm filter. Images were captured using a scientific camera controlled by μmanager or Metamorph software (Molecular Devices) and were analyzed with ImageJ.
Plant L.D., Xiong D., Romero J., Dai H, & Goldstein S.A. (2020). Hypoxia Produces Pro-arrhythmic Late Sodium Current in Cardiac Myocytes by SUMOylation of NaV1.5 Channels. Cell reports, 30(7), 2225-2236.e4.
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2

Donor-decay Kinetics Imaging Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Donor-decay time-course was studied as before (Plant et al., 2010 (link)), using an Olympus inverted epi-fluorescence microscope. Cells were studied in a solution comprising (in mM): NaCl 130, KCl 4, MgCl2 1.2, CaCl2 2, HEPES 10, pH was adjusted to 7.4 with NaOH. mTFP1 was excited at 445 nm and the emission collected through a 470–500 nm bandpass filter, YFP was excited at 514 nm and the emission collected through a 525–575 nm filter. Images were captured using a scientific camera controlled by μmanager or Metamorph software (Molecular Devices) and were analyzed with ImageJ.
Plant L.D., Xiong D., Romero J., Dai H, & Goldstein S.A. (2020). Hypoxia Produces Pro-arrhythmic Late Sodium Current in Cardiac Myocytes by SUMOylation of NaV1.5 Channels. Cell reports, 30(7), 2225-2236.e4.
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