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Ultrapure ligase l603 hc l

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Ultrapure ligase (L603-HC-L) is a laboratory enzyme used for DNA ligation. It catalyzes the formation of a phosphodiester bond between the 3' hydroxyl and 5' phosphate termini of DNA fragments, allowing for the joining of DNA molecules.

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Lab products found in correlation

Hiseq 2000, by Illumina (2 mentions) Ampure xp bead, by Beckman Coulter (2 mentions) Iproof, by Bio-Rad (2 mentions) End repair kit, by Illumina (2 mentions) Paired end adapters, by Qiagen (2 mentions) Pe 102 1003, by Qiagen (2 mentions) Dneasy blood and tissue kit, by Covaris (2 mentions)

2 protocols using ultrapure ligase l603 hc l

1

Illumina Sequencing Library Preparation

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DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit and sheared using a Covaris E220 (Woburn, MA) (duty cycle 10, intensity 5, cycles/burst 200 and time 180 s) to ~400 base pair (bp). Sheared genomic DNA was gel purified and used as input for Illumina sequencing library prep, with end-repair using the NEBNext end-repair kit (E6050L), and A-tailing with Taq polymerase. Ligation to Illumina paired-end adapters (PE‐102-1003) was done using Ultrapure ligase (L603-HC-L) from Enzymatics, and amplification with iProof (Bio-Rad). Agencourt Ampure XP beads were used for clean-up and size selection at each step. The resulting DNA library was sequenced on a single lane of an Illumina HiSeq2000 at the Stanford Center for Genomics and Personalized Medicine.
Kelley J.L., Peyton J.T., Fiston-Lavier A.S., Teets N.M., Yee M.C., Johnston J.S., Bustamante C.D., Lee R.E, & Denlinger D.L. (2014). Compact genome of the Antarctic midge is likely an adaptation to an extreme environment. Nature Communications, 5, 4611.
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2

DNA Extraction, Shearing, and Library Prep

Check if the same lab product or an alternative is used in the 5 most similar protocols
DNA was extracted using the Qiagen DNeasy Blood and Tissue Kit and sheared using a Covaris E220 (Woburn, MA) (duty cycle 10, intensity 5, cycles/burst 200, time 180 sec) to approximately 400 base pair (bp). Sheared genomic DNA was gel purified and used as input for Illumina sequencing library prep, with end-repair with NEBNext end-repair kit (E6050L), and A-tailing with Taq polymerase. Ligation to Illumina paired-end adapters (PE-102-1003) was done using Ultrapure ligase (L603-HC-L) from Enzymatics, and amplification with iProof (BioRad). Agencourt Ampure XP beads were used for clean-up and size selection at each step. The resulting DNA library was sequenced on a single lane of an Illumina HiSeq2000 at the Stanford Center for Genomics and Personalized Medicine.
Kelley J.L., Peyton J.T., Fiston-Lavier A.S., Teets N.M., Yee M.C., Johnston J.S., Bustamante C.D., Lee R.E, & Denlinger D.L. (2014). Compact genome of the Antarctic midge is likely an adaptation to an extreme environment. Nature Communications, 5, 4611.
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