Methomyl

Manufactured by Chem Service

Sourced in United States

Methomyl is a chemical compound used as a laboratory equipment. It is a white crystalline solid. Methomyl is commonly used in various chemical and research applications.

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Lab products found in correlation

Permethrin, by Chem Service (1 mentions) Aldicarb, by Merck Group (1 mentions)

2 protocols using methomyl

Insecticide Efficacy Testing Protocol

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The CAR21 susceptible strain of M. domestica used in this study was obtained from the USDA-ARS Center for Medical, Agricultural, and Veterinary Entomology (CMAVE). All the flies were 3–5-day-old adults during testing and were allowed to feed on sucrose and water ad libitum. The Orlando strain of Ae. aegypti was also obtained from USDA-ARS-CMAVE and reared under standard laboratory rearing protocols. l-menthone (97%), l-fenchone (>98%), l-carvone (98%) (hereafter referred to as “menthone”, “fenchone”, and “carvone”), and all chemicals for the acetylcholinesterase inhibition assay were obtained from Fisher Scientific (Waltham, MA, USA). Doses were formulated utilizing the densities of each monoterpenoid at 25 °C (i.e., room temp); l-carvone 0.96 g/mL, l-menthone 0.895 g/mL, and l-fenchone 0.948 g/mL. Permethrin (99.7% pure, 77.8% trans, and 21.9% cis) and methomyl (99.5% purity) were from Chem Service (West Chester, PA, USA).

Baker O.S., Norris E.J, & Burgess ER I.V. (2023). Insecticidal and Synergistic Potential of Three Monoterpenoids against the Yellow Fever Mosquito, Aedes aegypti (Diptera: Culicidae), and the House Fly, Musca domestica (Diptera: Muscidae). Molecules, 28(7), 3250.

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Reactivation of Carbamate-Inhibited Cholinesterases

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The challenge materials (CMs) were aldicarb (Sigma-Aldrich, St Louis, MO), methomyl (ChemService, West Chester, PA), and sarin (U.S. Army Edgewood Chemical Biological Center, Edgewood, MD). Sarin was used as a positive control challenge material for the reactivation experiments. The therapeutics used were atropine (free base) at 1.64 mg/mL in an aqueous solution, pH 4.3, and pralidoxime chloride (2-PAM Cl) at 102.8 mg/mL in an aqueous solution, each procured from King Pharmaceuticals (St. Louis, MO). The challenge and test materials used in the study are summarized in Table 1. The carbamates were diluted in multisol (a biocompatible solution of 48.5% water, 40% propylene glycol, 10% ethanol, and 1.5% benzyl alcohol, v/v).

Brittain M.K., McGarry K.G., Moyer R.A., Babin M.C., Jett D.A., Platoff GE J.r, & Yeung D.T. (2016). Efficacy of Recommended Pre-Hospital Human Equivalent Doses of Atropine and Pralidoxime against the Toxic Effects of Carbamate Poisoning in the Hartley Guinea Pig. International journal of toxicology, 35(3), 344-357.

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