Rat monoclonal peroxidase conjugated anti ha 3f10

Manufactured by Roche

Rat monoclonal peroxidase-conjugated anti-HA (3F10) is a laboratory reagent used for detecting and identifying proteins that have been tagged with the HA (Hemagglutinin) epitope. It consists of a rat-derived monoclonal antibody that is conjugated to the enzyme peroxidase. This product can be used in various immunoassay techniques to specifically recognize and bind to HA-tagged proteins.

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Lab products found in correlation

Rabbit polyclonal anti flag, by Merck Group (1 mentions) Mouse monoclonal anti flag m2, by Merck Group (1 mentions) Mouse monoclonal gfp, by Santa Cruz Biotechnology (1 mentions) Anti c myc, by Abcam (1 mentions)

Close spelling variants of this product

Rat monoclonal anti-HA (3F10) Rat anti-HA 3F10 Anti-HA Peroxidase (3F10; Rat monoclonal anti-HA Rat anti-HA-Peroxidase Anti-HA (3F10, HA (3F10; Rat anti-HA Anti-HA-peroxidase Monoclonal rat

2 protocols using rat monoclonal peroxidase conjugated anti ha 3f10

Antibody sources for yeast protein detection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit polyclonal anti-Cse4p (Pinsky et al. 2003 (link)) and rabbit polyclonal anti-Sam35p (Chan and Lithgow 2008 (link)) were generous gifts from Sue Biggins and Trevor Lithgow, respectively. Commercial antibodies used were: mouse monoclonal Cox1p, mouse monoclonal Hsp60p, mouse monoclonal Porin and rabbit polyclonal Prc1p (all from abcam); mouse monoclonal myc (9E10) and mouse monoclonal green fluorescent protein (GFP; both from Santa Cruz Biotechnology); mouse monoclonal phosphoglycerate kinase (Pgk1p; Life Technologies); and rat monoclonal peroxidase-conjugated anti-HA (3F10; Roche).

Metzger M.B., Scales J.L., Dunklebarger M.F, & Weissman A.M. (2017). The Ubiquitin Ligase (E3) Psh1p Is Required for Proper Segregation of both Centromeric and Two-Micron Plasmids in Saccharomyces cerevisiae. G3: Genes|Genomes|Genetics, 7(11), 3731-3743.

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Antibodies for Protein Detection

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Rabbit polyclonal anti-Sam35 (Chan and Lithgow, 2008 (link)) was a generous gift from Trevor Lithgow. Rabbit polyclonal anti-Sis1 (Yan and Craig, 1999 (link)) was a generous gift from Elizabeth Craig. Rabbit polyclonal anti-Cue1 and anti-ubiquitin were described previously (Kostova et al., 2009 (link)). Commercial antibodies used were: mouse monoclonal PORIN (MTCO1; abcam); rabbit polyclonal Prc1 (CPY; abcam); mouse monoclonal GFP (Santa Cruz Biotechnology); mouse monoclonal phosphoglycerate kinase 1 (PGK; Life Technologies); rat monoclonal peroxidase-conjugated anti-HA (3F10; Roche); mouse monoclonal anti-FLAG (M2; Sigma-Aldrich); rabbit polyclonal anti-FLAG (Sigma-Aldrich); and rabbit polyclonal anti-c-myc (Abcam).

Metzger M.B., Scales J.L., Dunklebarger M.F., Loncarek J, & Weissman A.M. (2020). A protein quality control pathway at the mitochondrial outer membrane. eLife, 9, e51065.

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