BioPlex 2200 (Bio-Rad, Hercules, CA) system is an automated analyzer that uses multiplex bead technology (Luminex, Austin, TX, USA) to simultaneously detect antibodies to several antigens in a single tube. The BioPlex 2200 ANA Screen is intended for the qualitative screening of ANA, the quantitative detection of antibody to dsDNA, and the semiquantitative detection of ten separate antibodies (Chromatin, Ribosomal P, SS-A, SS-B, Sm, SmRNP, RNP, Scl-70, Jo-1, and Centromere B) [11 (link), 12 (link)] in human serum and/or EDTA or heparinized plasma. The test system is used as an aid in the diagnosis of SARD. Characteristics of the assay are summarized in Table 1 .
Multiplex bead technology
Manufactured by DiaSorin
Sourced in United States
Multiplex bead technology is a laboratory equipment that enables the simultaneous detection and quantification of multiple analytes in a single sample. It utilizes color-coded beads coated with specific capture reagents, allowing for the efficient and high-throughput analysis of various biomolecules, such as proteins, cytokines, or nucleic acids.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using multiplex bead technology
1
Multiplex Bead-Based ANA Screening
2
Autoantibody Analysis in Clinical Lab
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All blood and urine chemistry analysis was performed according to standard routine at the time of inclusion at the internationally certified Karolinska University Hospital laboratory. ANA were analyzed by indirect immunofluorescence on HEp-2 cells (Immuno Concepts, Sacramento, CA, USA). Antibodies to specific nuclear antigens (anti-dsDNA, antinucleosomes, anti-Ro52/SSA, anti-Ro60/SSA, anti-La/SSB, anti-Smith) and phospholipids (anticardiolipin antibodies immunoglobulin (aCL IgG), and anti-β2-antiglycoprotein domain 1 antibodies IgG [aβ2GP1IgG]) were analyzed by multiplex bead technology (Luminex, Austin, TX, USA) using the BioPlex 2200 system (Bio-Rad Laboratories, Hercules, CA, USA). The cutoff for aCL and aβ2GP1 fulfilled the 99th percentile as described previously [31 (link)]. Lupus anticoagulant (LA) was determined by the modified dilute Russell’s viper venom time method (Biopool, Umeå, Sweden) using Bioclot LA (Trinity Biotech, Co.Wicklow, Ireland). aCL, aβ2GP1, and LA are together referred to as antiphospholipid antibodies (aPL).
3
Cytokine Profiling in pHCECs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Concentrations of the proinflammatory cytokines IL-6 and TNF-a in the supernatants of pHCECs cultured for 24 hours in normal or high glucose-added medium with or without NAC were determined using a custom designed cytokine/chemokine kit that uses multiplex bead technology (Luminex, Inc., Austin, TX, USA). Assays were conducted in accordance with the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!